Ebolavirus comparative genomics

Jun, Se-Ran; Leuze, Michael R.; Nookaew, Intawat; Uberbacher, Edward C.; Land, Miriam; Zhang, Qian; Wanchai, Visanu; Chai, Juanjuan; Nielsen, Morten; Trolle, Thomas; Lund, Ole; Buzard, Gregory S.; Pedersen, Thomas D.; Wassenaar, Trudy M.; Ussery, David W.

doi:10.1093/femsre/fuv031

DOE OSTI · 1265422

Ebolavirus comparative genomics

Jun, Se-Ran·Leuze, Michael R.·Nookaew, Intawat·Uberbacher, Edward C.·Land, Miriam·Zhang, Qian·Wanchai, Visanu·Chai, Juanjuan·Nielsen, Morten·Trolle, Thomas·Lund, Ole·Buzard, Gregory S.·Pedersen, Thomas D.·Wassenaar, Trudy M.·Ussery, David W.

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Abstract

The 2014 Ebola outbreak in West Africa is the largest documented for this virus. We examine the dynamics of this genome, comparing more than one hundred currently available ebolavirus genomes to each other and to other viral genomes. Based on oligomer frequency analysis, the family Filoviridae forms a distinct group from all other sequenced viral genomes. All filovirus genomes sequenced to date encode proteins with similar functions and gene order, although there is considerable divergence in sequences between the three genera Ebolavirus, Cuevavirus, and Marburgvirus within the family Filoviridae. Whereas all ebolavirus genomes are quite similar (multiple sequences of the same strain are often identical), variation is most common in the intergenic regions and within specific areas of the genes encoding the glycoprotein (GP), nucleoprotein (NP), and polymerase (L). We predict regions that could contain epitope-binding sites, which might be good vaccine targets. In conclusion, this information, combined with glycosylation sites and experimentally determined epitopes, can identify the most promising regions for the development of therapeutic strategies.

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59 BASIC BIOLOGICAL SCIENCES Ebola Virus Ebola virus disease (EVD)Filovirus comparative genomics epitope prediction viral genomes

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Jun, Se-Ran, Leuze, Michael R., Nookaew, Intawat, Uberbacher, Edward C., Land, Miriam, Zhang, Qian, Wanchai, Visanu, Chai, Juanjuan, Nielsen, Morten, Trolle, Thomas, Lund, Ole, Buzard, Gregory S., Pedersen, Thomas D., Wassenaar, Trudy M., Ussery, David W.. 2015-07-14. Ebolavirus comparative genomics. https://doi.org/10.1093/femsre%2Ffuv031

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