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DOE OSTI · 2530263

Reveal-CoV Diaagnostic Platform (Final Report)

Dugan, L. C.
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Abstract

LLNL designed and built a rapid, RT-LAMP-based molecular diagnostics platform as a potential tool to quickly diagnose COVID-19 in under one hour. This point-of-care testing approach involves an initial high temperature swab sample inactivation step followed by amplification of viral RNA using up to 5 control and pathogen-specific assays. Results are determined based on a discreet reaction color change from red to yellow but can also be determined using fluorescence detection. Testing of this prototype platform was conducted with synthetic viral RNA and dried, stabilized reagents. Buffer systems, swab selection, and assay stabilization formulations were evaluated for performance. Limits of detection were determined using RNA; however, testing was not performed with viable SARS-CoV-2 virus or clinical samples.

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59 BASIC BIOLOGICAL SCIENCESBiological and medical sciences

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BibTeXRIS

Dugan, L. C.. 2021-05-14. Reveal-CoV Diaagnostic Platform (Final Report). https://doi.org/10.2172/2530263

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