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Characterization of a monoclonal antibody specific to California serogroup orthobunyaviruses and development as a chimeric immunoglobulin M-positive control in human diagnostics

ABSTRACT California serogroup viruses (CSGVs) of medical importance in the United States include La Crosse virus, Jamestown Canyon virus (JCV), California encephalitis virus, and snowshoe hare virus. Current diagnosis of CSGVs relies heavily on serologic techniques for detecting immunoglobulin M (IgM), an indication of a recent CSGV infection. However, human-positive control sera reactive to viruses in the serogroup are scarce because detection of recent infections is rare. Here, we describe the development of new murine monoclonal antibodies (MAbs) reactive to CSGVs and the engineering of a human-murine chimeric antibody by combining the variable regions of the broadly CSGV cross-reactive murine MAb, 3-3B6/2-3B2 and the constant region of the human IgM. MAb 3-3B6/2-3B2 recognizes a tertiary epitope on the Gn/Gc heterodimer, and epitopes important in JCV neutralization were mapped to the Gc glycoprotein. This engineered human IgM constitutively expressed in a HEK-293 stable cell line can replace human-positive control sera in diagnostic serological techniques such as IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA). Compared to the parent murine MAbs, the human-chimeric IgM antibody had identical serological activity to CSGVs in ELISA and demonstrated equivalent reactivity compared to human immune sera in the MAC-ELISA. Importance Orthobunyaviruses in the California serogroup cause severe neurological disease in children and adults. While these viruses are known to circulate widely in North America, their occurrence is rare. Serological testing for CSGVs is hindered by the limited availability and volumes of human-positive specimens needed as controls in serologic assays. Here, we described the development of a murine monoclonal antibody cross-reactive to CSGVs engineered to contain the variable regions of the murine antibody on the backbone of human IgM. The chimeric IgM produced from the stably expressing HEK293 cell line was evaluated for use as a surrogate human-positive control in a serologic diagnostic test.

Microbiology↗

Hydrogen Mitigation Process Installation at Nevada Solar One

The National Renewable Energy Laboratory (NREL) and Acciona Solar Power (ASP) have developed and are implementing a process that addresses the issue of hydrogen buildup in parabolic trough power plants. Our method selectively removes hydrogen from the expansion tanks of the power plant to control hydrogen levels in the circulating heat-transfer fluid (HTF). During previous work, we developed a sensor that measures hydrogen partial pressure in the expansion-tank headspace gas. We demonstrated that our sensor measures hydrogen levels over a wide range of partial pressure—from 1.33 mbar down to 0.003 mbar. More recently, we conceived and developed an integrated process module that performs both hydrogen sensing and separating functions. The sensor/separator measures hydrogen partial pressure in the headspace gas in the same way as our original sensor design. Additionally, the integrated module separates hydrogen from the headspace gas to reduce hydrogen to the level needed to maintain the performance of receivers in the collector field. We demonstrated the performance of a laboratory-scale version of this module. Testing showed that the module performed as expected: the accuracy of the sensing function was ±7%, and the hydrogen extraction rate for the separating mode was consistent with our modeling predictions. The primary benefit of this module is its simple design, both in terms of function and incorporation into the HTF subsystem of the power plant. Most recently, NREL and ASP planned, specified, and designed a mitigation process that is based on the integrated module. We are currently completing installation of this process at ASP's Nevada Solar One power plant in Boulder City, Nevada, USA. The mitigation process is being installed at ground level below the HTF expansion tanks, where it draws headspace gas from the tanks, removes hydrogen, and returns the treated gas back to the tanks. In this paper, we report progress on the installation and describe some of the many design details and challenges that we addressed during the past year. We will generate initial performance data from the Nevada Solar One mitigation process in early 2020.

41 EE - Solar Energy Technologies Office (EE-4S)↗

The North Pacific Summer Jet and Climate Extremes over North America: Mechanisms and Model Biases

The North Pacific summer jet (NPSJ) plays a critical role as a waveguide for weather systems and other sub-seasonal Rossby waves entering North America and therefore has a controlling influence on the warm season weather and climate extremes over much of the continent. In particular, much of the warm season precipitation that occurs over the central United States depends on subseasonal transients that are able to tap moisture from the Gulf of Mexico as they propagate across the continent. The GEOS-5 atmospheric general circulation model (AGCM), like many AGCMs, is deficient in the simulation of the NPSJ. It is shown that the deficiency is composed of: 1) a stunted jet in which the strongest winds are confined to the Asian continent, failing to extend across the North Pacific into the Gulf of Alaska as observed, and 2) a zonally symmetric poleward shift in the jet. These biases combine to impede the eastward propagation of the weather systems into the continent (the stunted jet), and deprive those systems that do enter the continent access to the moisture from the Gulf (the northward shift), leading to a dry bias over the central US. It is shown that the stunted jet bias is the result of too strong heating that occurs just south of the jet core over and near Tibet. Furthermore, it is shown that the poleward shift of the NPSJ can be corrected in the current GEOS-5 AGCM by increasing the vertical resolution. The implications of these results for improving warm season forecasts of extreme events will be discussed.

NPS↗

Interactive Visualization of High-Dimensional Petascale Ocean Data

We describe an application for interactive visualization of 5 petabytes of time-varying multivariate data from a high-resolution global ocean circulation model. The input data are 10311 hourly (ocean time) time steps of various 2D and 3D fields from a 22-billion point 1/48- degree “lat-lon cap” configuration of the MIT General Circulation Model (MITgcm). We map the global horizontal model domain onto our 128-screen (8x16) tiled display wall to produce a canonical tiling with approximately one MITgcm grid point per display pixel, and using this tiling we encode the entire time series for multiple native and computed scalar quantities at a collection of ocean depths. We reduce disk bandwidth requirements by converting the model’s floating point data to 16-bit fixed point values, and compressing those values with a lossless video encoder, which together allow synchronized playback at 24 time steps per second across all 128 displays. The application allows dynamic assignment of any two encoded tiles to any display, and has multiple interfaces for quickly specifying various orderly arrangements of tiles. All subsequent rendering is done on the fly, with run time control of colormaps, transfer functions, histogram equalization, and labeling. The two data streams on each screen can be rendered independently and combined in various ways, including blending, differencing, horizontal/ vertical wipes, and checkerboarding. The two data streams on any screen can optionally be displayed as a scatterplot in their joint attribute space. All scatterplots and map-view plots from the same x/y location and depth are linked so they all show the current brushable selection. Ocean scientists have used the system, and have found previously unidentified features in the data.

Interactive↗

Portable Apparatus for Electrochemical Sensing of Ethylene

A small, lightweight, portable apparatus based on an electrochemical sensing principle has been developed for monitoring low concentrations of ethylene in air. Ethylene has long been known to be produced by plants and to stimulate the growth and other aspects of the development of plants (including, notably, ripening of fruits and vegetables), even at concentrations as low as tens of parts per billion (ppb). The effects are magnified in plant-growth and -storage chambers wherein ethylene can accumulate. There is increasing recognition in agriculture and related industries that it is desirable to monitor and control ethylene concentrations in order to optimize the growth, storage, and ripening of plant products. Hence, there are numerous potential uses for the present apparatus in conjunction with equipment for controlling ethylene concentrations. The ethylene sensor is of a thick-film type with a design optimized for a low detection limit. The sensor includes a noble metal sensing electrode on a chip and a hydrated solid-electrolyte membrane that is held in contact with the chip. Also located on the sensor chip are a counter electrode and a reference electrode. The sensing electrode is held at a fixed potential versus the reference electrode. Detection takes place at active-triple-point areas where the sensing electrode, electrolyte, and sample gas meet. These areas are formed by cutting openings in the electrolyte membrane. The electrode current generated from electrochemical oxidation of ethylene at the active triple points is proportional to the concentration of ethylene. An additional film of the solid-electrolyte membrane material is deposited on the sensing electrode to increase the effective triple-point areas and thereby enhance the detection signal. The sensor chip is placed in a holder that is part of a polycarbonate housing. When fully assembled, the housing holds the solid-electrolyte membrane in contact with the chip (see figure). The housing includes a water reservoir for keeping the solid-electrolyte membrane hydrated. The housing also includes flow channels for circulating a sample stream of air over the chip: ethylene is brought to the sensing surface predominately by convection in this sample stream. The sample stream is generated by a built-in sampling pump. The forced circulation of sample air contributes to the attainment of a low detection limit.

Manoukian, Mourad↗

Atmospheric Constraints on Landing Site Selection

The Martian atmosphere is a significant part of the environment that the Mars Exploration Rovers (MER) will encounter. As such, it imposes important constraints on where the rovers can and cannot land. Unfortunately, as there are no meteorological instruments on the rovers, there is little atmospheric science that can be accomplished, and no scientific preference for landing sites. The atmosphere constrains landing site selection in two main areas, the entry descent and landing (EDL) process and the survivability of the rovers on the surface. EDL is influenced by the density profile and boundary layer winds (up to altitudes of 5 to 10 km). Surface survivability involves atmospheric dust, temperatures and winds. During EDL, the atmosphere is used to slow the lander down, both ballistically and on the parachute. This limits the maximum elevation of the landing site to -1.3 km below the MOLA reference aeroid. The landers need to encounter a sufficiently dense atmosphere to be able to stop, and the deeper the landing site, the more column integrated atmosphere the lander can pass through before reaching the surface. The current limit was determined both by a desire to be able to reach the hematite region and by a set of atmosphere models we developed for EDL simulations. These are based on Thermal Emission Spectrometer (TES) atmospheric profile measurements, Ames Mars General Circulation Model (MGCM) results, and the 1-D Ames GCM radiative/convective model by J. Murphy. The latter is used for the near surface diurnal cycle. The current version of our model encompasses representative latitude bands, but we intend to make specific models for the final candidate landing sites to insure that they fall within the general envelope. The second constraint imposed on potential landing sites through the EDL process is the near surface wind. The wind in the lower approximately 5 km determines the horizontal velocity that the landers have when they land. Due to the mechanics of the landing process, the total velocity (including both the horizontal and vertical components) determines whether or not the landers are successful. Unfortunately, the landing system has no easy way to nullify any horizontal velocity imparted by the wind, so the landing sites selected need to have as little wind as possible. In addition to the mean wind velocity, the landing system is sensitive to vertical wind shear in the lowest kilometer or so. Wind shear can deflect the retro rockets (RADs) from their nominal vertical orientation producing unwanted horizontal spacecraft velocities. Both mean velocity and wind shear are dominated by the the local topography and other surface properties (in particular albedo and thermal inertia which control the surface temperature). This is seen even in simplified 2-D mesoscale models. The effects in a fully 3-D model are expected to he even more topographically dependent. In particular there is potential for wind channeling in canyons and other terrain features. Boundary layer winds and wind shear are currently being modeled based on terrestrial data and boundary layer scaling laws modified for Martian conditions. We hope to supplement this with mesoscale model results (from several sources) once the number of landing sites is reduced to a manageable number.

Kass, David M.↗

High-Resolution Specification of the Land and Ocean Surface for Improving Regional Mesoscale Model Predictions

The exchange of energy and moisture between the Earth's surface and the atmospheric boundary layer plays a critical role in many meteorological processes. High-resolution, accurate representations of surface properties such as sea-surface temperature (SST), soil temperature and moisture content, ground fluxes, and vegetation are necessary to better understand the Earth-atmosphere interactions and improve numerical predictions of sensible weather. The NASA Short-term Prediction Research and Transition (SPoRT) Center has been conducting separate studies to examine the impacts of high-resolution land-surface initialization data from the Goddard Space Flight Center Land Information System (LIS) on subsequent WRF forecasts, as well as the influence of initializing WRF with SST composites derived from the MODIS instrument. This current project addresses the combined impacts of using high-resolution lower boundary data over both land (LIS data) and water (MODIS SSTs) on the subsequent daily WRF forecasts over Florida during May 2004. For this experiment, the WRF model is configured to run on a nested domain with 9- km and 3-kin grid spacing, centered on the Florida peninsula and adjacent coastal waters of the Gulf of Mexico and Atlantic Ocean. A control configuration of WRF is established to take all initial condition data from the NCEP Eta model. Meanwhile, two WRF experimental runs are configured to use high-resolution initialization data from (1) LIS land-surface data only, and (2) a combination of LIS data and high-resolution MODIS SST composites. The experiment involves running 24-hour simulations of the control WRF configuration, the MS-initialized WRF, and the LIS+MODIS-initialized WRF daily for the entire month of May 2004. All atmospheric data for initial and boundary conditions for the Control, LIS, and LIS+MODIS runs come from the NCEP Eta model on a 40-km grid. Verification statistics are generated at land surface observation sites and buoys, and the impacts of the high-resolution lower boundary data on the development and evolution of mesoscale circulations such as sea and land breezes are examined, This paper will present the results of these WRF modeling experiments using LIS and MODIS lower boundary datasets over the Florida peninsula during May 2004.

Case, Jonathan L.↗

Cryosphere and climate

This chapter will discuss two main issues related to the cryosphere and climate. One is the effect of sea ice and salinity gradients on ocean circulation, and in particular the possible role of sea ice transport on the ocean conveyer belt. The other is the effect of the cryosphere on climate, and in particular in high-latitude warming under increased CO2. In understanding the role of the cryosphere in both cases, it is useful to elucidate two types of toy sea ice models. Neither of these represents reality, but both are useful for illustrating the archetypal features of sea ice that control much of its large-scale behavior. The first model is a simple slab thermodynamic sea ice model as presented by Thorndike. In this model there are no dynamical effects and the thickness of ice is determined by surface heat budget and oceanic heat flux considerations, with the thickness of the ice critically affecting the effective conductivity whereby heat is transferred from the bottom ice boundary to the upper ice boundary. In this model all of the sea ice characteristics are controlled by the vertical heat fluxes from the atmosphere and ocean into the ice. The thickness is controlled by the ice's becoming an effective insulator as it thickens, thus reducing conductive heat loss to the atmosphere. A second model emphasizes the effects of dynamics. It considers the ice pack to be a collection of floes moving in response to synoptic wind fields and ocean currents. These motions create semipermanent leads (open areas) over which ice can grow rapidly.

Hibler, William D., III↗

Residential Building Energy Efficiency Field Studies: Low-Rise Multifamily

In recent years, the U.S. Department of Energy (DOE) has conducted a series of research studies to validate energy efficient building technologies in the field. Much of the work has focused on single-family construction, and some has also addressed commercial energy codes. The work detailed in this DOE-funded study (EE0007616) focuses on low-rise multifamily buildings (three stories or fewer above grade) in various regions of the United States, and reports on how state-level building codes are being implemented, both in terms of observed characteristics and also in terms of estimated energy impacts. Nearly 100 buildings across four states—Illinois, Minnesota, Oregon, and Washington—were sampled, which represent a range of climate types from mild temperature to very cold continental. Both common entry and outdoor entry buildings were included, and a parallel research project evaluated envelope air tightness and current still-evolving air tightness testing methods. Finally, a set of structured interviews of building designers and other relevant professionals was carried to out to gain more insight into this market. To the greatest extent possible, the methodology developed under the project for low-rise multifamily buildings mirrored the approach established by Pacific Northwest National Laboratory (PNNL) for single-family residential buildings (https://www.energy.gov/eere/buildings/downloads/residential-building-energy-code-field-study). This included the general approach to sampling, recruitment, and data collection, as well as data analysis and presentation. The range of permitting dates for the sites encompassed two energy code cycles in most regions. All states in the study had adopted a variation of the International Energy Conservation Code (IECC) for the structure of their state code. The low-rise multifamily occupancy presents a hybrid building type: most of the building’s conditioned floor area was covered by the residential chapter of the code while portions of the building (such as corridors and common spaces) fell under the commercial code chapter. The key items assessed in this work were: Building Shell—exterior wall insulation, ceiling insulation, foundation insulation, windows. Common Areas—HVAC and lighting. Living Units—lighting, ventilation. A few items were not assessed in detail, given their relative paucity in this occupancy type; these included duct leakage, pipe insulation, and hot water circulation controls. Building characteristics were collected via a combination of architectural, mechanical, electrical, and plumbing plan reviews and field inspections, and entered into a spreadsheet-based tool that was later queried to build a database. Data went through quality control both upon arrival and via a later semi-automated review and assurance process. Most of the data are presented graphically so that the reader can quickly assess compliance with the applicable energy codes (both by state and by code year). As a final step, EnergyPlus™ simulations were created for all buildings in the study to estimate both the as-found energy use intensity (EUI) and the energy and CO 2 that could be saved if features that were found to not meet code minimums were brought up to code. The savings estimates were tabulated for each of the four states in the study. The research team found that the single-family approach was largely applicable to low-rise multifamily buildings. This applies to both the data collection and the prototype EUI analysis. Most of the occupied space is living units and falls under residential energy codes, and many characteristics use similar envelope construction and relatively straightforward mechanical systems and lighting. One of the most challenging aspects of this work was to build an effective spreadsheet-based data collection instrument that could allow efficient collection of both building plan and field data. The research team is of the view that other methods could be equally effective if the work is done carefully with diligent quality control. The primary findings for the work center around the thermal envelope and mechanical systems and lighting at the sites: For thermal envelope components, the majority of buildings met or were better than the prescriptive code.This suggests that building designers and builders are aware of code requirements. In some cases, surveyed buildings were designed to qualify for energy efficiency certification programs. These buildings made up at least 20% of sampled buildings in each state. Almost all buildings met mechanical system efficiency requirements (for both living units and common areas). In some cases, sites employed systems that were considerably more efficient than required by the applicable energy code. Dwelling units had a majority of high-efficacy lighting, often in excess of the state’s residential code requirements. While high-efficacy fixtures were also typical in common areas (corridors and stairwells), lighting power densities (LPDs) in these areas were sometimes higher than levels dictated by the applicable part of the state commercial energy code. The simulation models run on a series of low-rise multifamily prototypes, informed by a composite of the field data collected, calculated annual EUIs of between 20 and 50 kBtu/ft2-yr, with the range representing the effects of both building characteristics and building location (climate zone). A detailed process (based on simulations of prototype buildings) was used to estimate the amount of avoided energy use that would occur if 100% adherence to energy codes were attained. The results indicated modest savings are attainable for items such as window thermal performance and common area lighting. The result is overall only a modest potential for additional energy savings, averaging about 10% of EUI.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Improving Global Reanalyses and Short Range Forecast Using TRMM and SSM/I-Derived Precipitation and Moisture Observations

Global reanalyses currently contain significant errors in the primary fields of the hydrological cycle such as precipitation, evaporation, moisture, and the related cloud fields, especially in the tropics. The Data Assimilation Office (DAO) at the NASA Goddard Space Flight Center has been exploring the use of tropical rainfall and total precipitable water (TPW) observations from the TRMM Microwave Imager (TMI) and the Special Sensor Microwave/ Imager (SSM/I) instruments to improve short-range forecast and reanalyses. We describe a "1+1"D procedure for assimilating 6-hr averaged rainfall and TPW in the Goddard Earth Observing System (GEOS) Data Assimilation System (DAS). The algorithm is based on a 6-hr time integration of a column version of the GEOS DAS, hence the "1+1"D designation. The scheme minimizes the least-square differences between the observed TPW and rain rates and those produced by the column model over the 6-hr analysis window. This 1+lD scheme, in its generalization to four dimensions, is related to the standard 4D variational assimilation but uses analysis increments instead of the initial condition as the control variable. Results show that assimilating the TMI and SSM/I rainfall and TPW observations improves not only the precipitation and moisture fields but also key climate parameters such as clouds, the radiation, the upper-tropospheric moisture, and the large-scale circulation in the tropics. In particular, assimilating these data reduce the state-dependent systematic errors in the assimilated products. The improved analysis also provides better initial conditions for short-range forecasts, but the improvements in forecast are less than improvements in the time-averaged assimilation fields, indicating that using these data types is effective in correcting biases and other errors of the forecast model in data assimilation.

Hou, Arthur Y.↗

Planning for Material Control and Accountancy at Liquid Fueled Molten Salt Reactors

The purpose of this report is to provide molten salt reactor (MSR) developers and future US Nuclear Regulatory Commission (NRC) license applicants with recommendations for developing an effective and practical material control and accounting (MC&A) plan, focused primarily on MSR designs that use circulating liquid fuel. Because of the breadth of MSR designs, there is no single, generic, detailed MC&A plan that will work for every design. The wide variation of fresh fuel salts, the method and frequency of loading fresh fuel, the reactor system design components (e.g., tanks, filtration systems, chemical processing streams), and waste streams will determine the specific measurement locations and instrumentation that can best meet MC&A objectives throughout an MSR facility. Additionally, MSR designs are rapidly evolving, and new design features and deployment scenarios that will affect MC&A are being explored and pursued. This report defines a generic MC&A approach that was developed for terrestrial (as opposed to maritime) deployments to meet the intent of NRC domestic safeguards and MC&A. MSR license applicants should consider nuclear safeguards (both domestic and international) and security throughout the design, as early as the preconceptual design phase. MC&A of special nuclear material (SNM) is an aspect of the NRC’s domestic safeguards program, alongside physical protection. Because liquid-fueled MSRs are reactors with SNM in nondiscrete (or item) form, it is likely that the NRC may require liquid-fueled MSR license applicants to submit a formal MC&A plan as a part of their license application. Currently, the NRC licensing protocol presents a challenge because the NRC MC&A regulations have not been updated to accommodate advanced reactors, including types of MSRs. Because no liquid-fueled MSR has been licensed for operation at the time of this report, no template or precedence for a successfully licensed MSR MC&A plan exists. However, the MSR license applicant can take advantage of the NRC’s published commitments to performance-based regulations. The authors recommend that the license applicant, or MSR designers, develop an MC&A plan throughout the design lifecycle and plan to submit a detailed MC&A program description, or MC&A plan, to the NRC as a part of a license application. No MC&A plan template or guidance exists that is specific to liquid-fueled MSRs. The authors recommend that license applicants discuss the topic of MC&A during preapplication engagement. Because of the uniqueness of MC&A for liquid fueled MSRs, the authors recommend that liquid fueled MSR developers engage with the NRC on the topic of MC&A in the early phases of its design development and follow up any time there are significant modifications in design plans that would affect MC&A. For example, topics like modifications in fuel handling processes, changes in uranium enrichment, or additional chemical processing streams added to the design could be discussed with the NRC specifically on the topic of MC&A.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Improving Global Reanalyses and Short-Range Forecast Using TRMM and SSM/I-Derived Precipitation and Moisture Observations

Global reanalyses currently contain significant errors in the primary fields of the hydrological cycle such as precipitation, evaporation, moisture, and the related cloud fields, especially in the tropics. The Data Assimilation Office (DAO) at the NASA Goddard Space Flight Center has been exploring the use of tropical rainfall and total precipitable water (TPW) observations from the TRMM Microwave Imager (TMI) and the Special Sensor Microwave/ Imager (SSM/I) instruments to improve short-range forecast and reanalyses. We describe a 1+1D procedure for assimilating 6-hr averaged rainfall and TPW in the Goddard Earth Observing System (GEOS) Data Assimilation System (DAS). The algorithm is based on a 6-hr time integration of a column version of the GEOS DAS, hence the 1+1D designation. The scheme minimizes the least-square differences between the observed TPW and rain rates and those produced by the column model over the 6-hr analysis window. This 1+1D scheme, in its generalization to four dimensions, is related to the standard 4D variational assimilation but uses analysis increments instead of the initial condition as the control variable. Results show that assimilating the TMI and SSW rainfall and TPW observations improves not only the precipitation and moisture fields but also key climate parameters such as clouds, the radiation, the upper-tropospheric moisture, and the large-scale circulation in the tropics. In particular, assimilating these data reduce the state-dependent systematic errors in the assimilated products. The improved analysis also provides better initial conditions for short-range forecasts, but the improvements in forecast are less than improvements in the time-averaged assimilation fields, indicating that using these data types is effective in correcting biases and other errors of the forecast model in data assimilation.

Hou, Arthur Y.↗

A Downscaling Analysis of the Urban Influence on Rainfall: TRMM Satellite Component AMS Conference on Satellite Meteorology and Oceanography

A recent publication by Shepherd et al. (2002) demonstrated the feasibility of using TRMM precipitation radar (PR) estimates to identify precipitation anomalies caused by urbanization. The approach is particularly useful for investigating this global process because TRMM data span large portions of the globe and comprise an extended temporal dataset. Recent literature suggests that urbanized regions of Houston, Texas may be influencing lightning and precipitation formation over and downwind of the city. Possible mechanisms include: (1) enhanced convergence through interactions between the sea breeze, Galveston bay breeze, and urban heat island circulations, (2) enhanced convergence due to increased surface roughness over the city and/or destabilization of the boundary layer by the UHI, or (3) enhanced cloud condensation nuclei due to urban and industrial aerosol sources. In this study, a downscaling analysis of spatial and temporal trends in rainfall around the Houston Area is being conducted. The downscaling analysis concept involves identifying and quantifying urban rainfall anomalies at progressively smaller spatial and temporal scales using the TRMM satellite, ground-based radar, and a dense network of rain gauges. The goal is to test the hypothesis that the Houston urban district and regions in the climatological downwind region of the city exhibit enhanced rainfall amounts relative to the climatological upwind regions. TRMM was launched in 1997 and currently operates in a low-inclination (35 deg), non-sun-synchronous orbit at an altitude of 402 km (350 km prior to August 2001). The satellite analysis follows the methodologies described in Shepherd et al. (2002). Nearly five years of TRMM PR-derived mean monthly rainfall estimates are utilized to produce annual and warm season isohyetal analyses around Houston. Early results indicate that rainfall rates (mm/h) for the entire period are largest within 100 km northeast and east of Houston (e.g. the "hypothesized downwind region"). The mean rainfall rate over the Houston urban center is 30.5% larger than the upwind control region. The mean rainfall rate in the downwind region is 34.4% larger than the upwind region. An analysis of a parameter called the urban rainfall ratio (URR) illustrates that 65% (88%) of the satellite-derived rainfall rates in the downwind (upwind control) region are greater (less) than the mean background rainfall rate of the entire study region. When the data is stratified by summer months from 1998 to 2001 (June-August), even greater influence over and downwind of the urban area is observed in the statistics. This result is consistent with published reports of urban-generated rainfall being more prevalent in the warm season. The research demonstrates that the evolving TRMM satellite climatology is a credible way to detect mesoscale precipitation signatures that may be linked to urbanization. Early results also corroborate recent findings on Houston-induced convection/drainfall anomalies. Burian and Shepherd will report on other aspects of the downscaling analysis in future forums, but early rain gauge results are consistent with the satellite-based observations.

Shepherd, J. Marshall↗

Biophysical model of eelgrass and water quality in Coos Bay, OR shows greater mitigation potential for ocean acidification than hypoxia

Seagrass beds provide important ecosystem services and are valued, in part, for their potential to mediate stressors such as ocean acidification and hypoxia (OAH) for sensitive species. However, the susceptibility of seagrasses to anthropogenic impacts and recent declines motivate the need to better understand the drivers of seagrass and the water quality consequences that occur with variation in seagrass abundance. To meet this need, we leveraged existing monitoring data (water quality and seagrass), hydrodynamic circulation model, and biogeochemical model framework with seagrass submodel, to produce a biophysical model of Coos Bay estuary, Oregon, U.S. The model includes biogeochemical processes involving water quality, plankton, seagrass, and sediment-water interactions. Ecosystem models like this are useful for evaluating complex estuarine systems because they allow us to extend our understanding of system dynamics beyond existing observations and perform experiments to identify the processes driving observed patterns. We used the biophysical model of Coos Bay to evaluate the dynamics of water quality and native eelgrass (Zostera marina) under three eelgrass abundance scenarios (zero eelgrass, current extent, and maximum observed extent) to elucidate the relationship between eelgrass and OAH. Including eelgrass in the Coos Bay model produced results that more closely resembled water quality observations - dissolved oxygen (DO) and pH were more dynamic in simulations with eelgrass, often having both higher highs and lower lows. While there were some areas of the estuary where DO improved with the addition of eelgrass to the model there was overall a small net increase in harmful DO conditions (based on a salmon physiological threshold). In contrast, ocean acidification conditions, pH and calcium carbonate saturation state for aragonite (Ω), were improved (based on oyster requirements) with the addition of eelgrass - although the magnitude of improvement differed seasonally and spatially. Our new model represents a useful tool - one which accounts for and controls the relevant physical and biogeochemical processes - to evaluate conditions that confer resilience or enhance vulnerability to OAH in an important Pacific Northwest coastal estuary and results can inform the OAH-related dynamics occurring in other eastern boundary current estuaries.

FVCOM-ICM↗

Satellite Radiation Products for Ocean Biology and Biogeochemistry: Needs, State-Of-The-Art, Gaps, Development Priorities, and Opportunities

Knowing the spatial and temporal distribution of the underwater light field, i.e., the spectral and angular structure of the radiant intensity at any point in the water column, is essential to understanding the biogeochemical processes that control the composition and evolution of aquatic ecosystems and their impact on climate and reaction to climate change. At present, only a few properties are reliably retrieved from space, either directly or via water-leaving radiance. Existing satellite products are limited to planar photosynthetically available radiation (PAR) and ultraviolet (UV) irradiance above the surface and diffuse attenuation coefficient. Examples of operational products are provided, and their advantages and drawbacks are examined. The usefulness and convenience of these products notwithstanding, there is a need, as expressed by the user community, for other products, i.e., sub-surface planar and scalar fluxes, average cosine, spectral fluxes (UV to visible), diurnal fluxes, absorbed fraction of PAR by live algae (APAR), surface albedo, vertical attenuation, and heating rate, and for associating uncertainties to any product on a pixel-by-pixel basis. Methodologies to obtain the new products are qualitatively discussed in view of most recent scientific knowledge and current and future satellite missions, and specific algorithms are presented for some new products, namely sub-surface fluxes and average cosine. A strategy and roadmap (short, medium, and long term) for usage and development priorities is provided, taking into account needs and readiness level. Combining observations from satellites overpassing at different times and geostationary satellites should be pursued to improve the quality of daily-integrated radiation fields, and products should be generated without gaps to provide boundary conditions for general circulation and biogeochemical models. Examples of new products, i.e., daily scalar PAR below the surface, daily average cosine for PAR, and sub-surface spectral scalar fluxes are presented. A procedure to estimate algorithm uncertainties in the total uncertainty budget for above-surface daily PAR, based on radiative simulations for expected situations, is described. In the future, space-borne lidars with ocean profiling capability offer the best hope for improving our knowledge of sub-surface fields. To maximize temporal coverage, space agencies should consider placing ocean-color instruments in L1 orbit, where the sunlit part of the Earth can be frequently observed.

Frouin, Robert↗

Beam Dynamics of the Muon $g\textrm{-}2$ Experiment

The Muon $g\textrm{-}2$ Experiment (E989) at Fermilab aims to measure the muon anomalous magnetic moment $a_{\mu}$ with unprecedented precision, potentially uncovering physics beyond the Standard Model of particle physics. The result based on Runs 1-3, released in 2023, achieved a precision of 0.20 ppm. The experiment circulates muons in a storage ring, measuring $a_{\mu}$ from decay positron time and energy measurements collected with calorimeters. To achieve the required accuracy, it is crucial to measure and control the magnetic field in the ring with high precision. Beam dynamics corrections are necessary for muons not orbiting exactly in the midplane, for their oscillations, and for electric field effects. Highly accurate beam dynamics simulations are instrumental for quantifying and validating the beam dynamics corrections, ultimately improving the precision of the $a_{\mu}$ measurement and facilitating the achievement of the ambitious $70\:\mathrm{ppb}$ systematic uncertainty goal. The measured field data was incorporated into models for simulations using three codes: \texttt{gm2ringsim} (an internal Geant4-based code), \textit{COSY INFINITY}, and \textit{BMAD}. The advantages of \texttt{gm2ringsim} include using CAD-based geometry and modelling the detector effects. \textit{COSY INFINITY} is a highly accurate and efficient code that uses high-order differential-algebraic transfer maps, precise fringe field calculations, and advanced symplectification methods. Symplectification is important for maintaining the physical correctness of the muon beam behaviour with high precision over the storage time, ensuring conservation of phase space volume and preventing artificial damping or excitation of particle motion. The experiment completed its final Run 6 in July 2023, collecting 21 times more data than the previous BNL experiment. Analyses of data from Runs 4-6 are ongoing, with results planned for release in 2025, potentially resolving the current tension between experiment and theory.

43 PARTICLE ACCELERATORS↗

Simulation of DWPF Chemical Process Cell Using a Reaction Calorimeter

The Defense Waste Processing Facility (DWPF) converts liquid radioactive waste into glass using a Sludge Receipt Adjustment Tank (SRAT) and Slurry Mix Evaporator (SME) in addition to other processes. The SRAT and SME processes can be simulated on a small-scale using the Mettler-Toledo Reaction Calorimeter (RC1). This equipment is able to automate the execution and monitoring of experiments involving nonradioactive chemical simulants. During the chemical processing of sludge in the SRAT process, foam begins to form and can foam over the reaction vessel. The current antifoam used at DWPF creates a risk of increased flammability and has a limited pH range. Usually, mercury is circulated back into the reaction vessel during the SRAT process, making it difficult to remove. To combat foam over, novel antifoams will be tested during the boil-up process using the RC1. To improve mercury extraction, 3D-printed coalescer columns will be tested within the Mercury Water Wash Tank (MWWT) of the RC1. Testing of Simulant Sludge Batch 6 (SB6) was performed to ensure: the sludge would form a stable foam at boiling during full SRAT/SME runs; the antifoam would reduce the amount of foam within the vessel The sludge and a Precipitate Reactor Feed Tank (PRFT) simulant with monosodium titanate (MST) were mixed separately, combined, and then heated to boiling. As shown below, the antifoam used was able to eliminate the foam within the vessel, as expected. The FormLabs Form2 3D Printer can be used to create roughly 3-inch tall and 1-inch diameter columns from clear and ceramic resins. The columns will utilize chemical and physical forces to draw the mercury out of a condensed offgas stream leaving the RC1 reaction vessel. These columns will be tested during full SRAT/SME runs on the basis of how well they are able to collect elemental mercury. In preparation for full-scale runs, the RC1 equipment was assembled onto a custom table and tested with water runs. The software used to control automation, iControl, has a user-friendly interface that allows the experiment to be altered as needed. An example of the data recorded during an experimental run is represented in Figure 1 below. Water runs were utilized as a way to prove if the RC1 system was functioning in the following areas: Boil-up rates, Chemical additions using pumps, Temperature readings, Balance readings, Probe readings, Offgas system, Agitation. Future Plans: Five novel antifoams will be tested during full SRAT/SME runs. The most effective antifoam will be utilized in DWPF's Chemical Process Cell. The design of the Mercury Extraction Coalescer can be further manipulated to increase the extraction of not only elemental mercury, but other heavy metals in environmental remediation processes as well.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Decoupling of Iron and Phosphate in the Global Ocean

Iron is an essential micronutrient for marine phytoplankton, limiting their growth in high nutrient, low chlorophyll regions of the ocean. I use a hierarchy of ocean circulation and biogeochemistry models to understand controls on global iron distribution. I formulate a mechanistic model of iron cycling which includes scavenging onto sinking particles and complexation with an organic ligand. The iron cycle is coupled to a phosphorus cycling model. Iron's aeolian source is prescribed. In the context of a highly idealized multi-box model scheme, the model can be brought into consistency with the relatively sparse ocean observations of iron in the oceans. This biogeochemical scheme is also implemented in a coarse resolution ocean general circulation model. This model also successfully reproduces the broad regional patterns of iron and phosphorus. In particular, the high macronutrient concentrations of the Southern Ocean result from iron limitation in the model. Due to the potential ability of iron to change the efficiency of the carbon pump in the remote Southern Ocean, I study Southern Ocean surface phosphate response to increased aeolian dust flux. My box model and GCM results suggest that a global ten fold increase in dust flux can support a phosphate drawdown of 0.25-0.5 micromolar.

GLOBAL OCEAN MODEL↗