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At least 235 records · Page 13

Multi-omics data resource: Data package 22 (Pck022)

In type 1 diabetes (T1D), autoimmune response and inflammation cause the death of pancreatic ß cells, leading to the body’s inability to produce insulin and maintain glucose homeostasis. This process is at least in part mediated by pro-inflammatory cytokines, such as interferon (IFN)a, IFN?, interleukin (IL)-1ß, and tumor necrosis factor (TNF)a, which induce ß-cell dysfunction and apoptosis. A deep understanding of the ß-cell signaling and regulatory networks induced by these cytokines could lead to the identification of therapeutic targets to prevent T1D development. To study cytokine-mediated islets/ß-cell signaling and regulatory networks, a variety of omics experiments have been conducted, including transcriptomics, epigenomics (DNA methylation, UMI-4C, ATAC-seq & ChIP-seq), proteomics (bottom-up, top-down, post-translational modification analysis), lipidomics, and metabolomics. The combination of these datasets can be instrumental in identifying signaling components and regulatory factors involved in ß-cell stress/death. Here, we aggregated these multiple omics datasets into a centralized location, providing a quality-controlled and statistically rigorous resource for investigators seeking to holistically study ß-cell regulation by pro-inflammatory cytokines. The data package consists of isolated pancreatic islets from adult male C57BL6/J mice treated with IL-1β, IFNγ or IL-1β + IFNγ for 6 h and submitted for scRNA-seq. This study focused on understanding the heterogeneity of the cytokine-mediated response. Data contributors: Jennifer S Stancill & John A Corbett: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA Data repository: GSE156175 Publication: 10.26508/lsa.202000949

Sarkar, Soumyadeep [Pacific Northwest National Lab↗

Multi-omics data resource: Data package 23 (Pck023)

The data package consists of isolated pancreatic islets from adult male C57BL6/J mice treated with IL-1β + IFNγ, IL-1β + IFNγ + NMMA, or NMMA alone for 18 h and submitted for scRNA-seq. This study focused on the cell-type-specific effects of nitric oxide signaling in islets and characterized the heterogeneity of responses. Data contributors: Jennifer S Stancill & John A Corbett: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA Data repository: GSE183010 Publication: 10.1093/function/zqab063

Sarkar, Soumyadeep [Pacific Northwest National Lab↗

Multi-omics data resource: Data package 24 (Pck024)

The data package consists of isolated pancreatic islets from 3 human donors treated with IL-1β, IFNγ or IL-1β + IFNγ for 6 h and IL-1β, IFNγ, IL-1β + IFNγ, IL-1β + IFNγ + NMMA or NMMA for 18 h and submitted for scRNA-seq. This study examines cytokine-stimulated changes in gene expression in human islets using single-cell RNA sequencing. Data contributors: Jennifer S Stancill & John A Corbett: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA Data repository: GSE251730 Publication: 10.1093/function/zqae015

Sarkar, Soumyadeep [Pacific Northwest National Lab↗

Data from: Associative nitrogen fixation (ANF) in switchgrass (Panicum virgatum) across a nitrogen input gradient

Associative N fixation (ANF), the process by which dinitrogen gas is converted to ammonia by bacteria in casual association with plants, has not been well-studied in temperate ecosystems. We examined the ANF potential of switchgrass (Panicum virgatum L.), a North American prairie grass whose productivity is often unresponsive to N fertilizer addition, via separate short-term 15N2 incubations of rhizosphere soils and excised roots four times during the growing season. Measurements occurred along N fertilization gradients at two sites with contrasting soil fertility (Wisconsin, USA Mollisols and Michigan, USA Alfisols). In general, we found that ANF potentials declined with long-term N addition, corresponding with increased soil N availability. Although we hypothesized that ANF potential would track plant N demand through the growing season, the highest root fixation rates occurred after plants senesced, suggesting that root diazotrophs exploit carbon (C) released during senescence, as C is translocated from aboveground tissues to roots for wintertime storage. Measured ANF potentials, coupled with mass balance calculations, suggest that ANF appears to be an important source of N to unfertilized switchgrass, and, by extension, to temperate grasslands in general.

09 BIOMASS FUELS↗

Observing low-altitude features in ozone concentrations in a shoreline environment via uncrewed aerial systems

Abstract. Ozone is a pollutant formed in the atmosphere by photochemical processes involving nitrogen oxides (NOx) and volatile organic compounds (VOCs) when exposed to sunlight. Tropospheric boundary layer ozone is regularly measured at ground stations and sampled infrequently through balloon, lidar, and crewed aircraft platforms, which have demonstrated characteristic patterns with altitude. Here, to better resolve vertical profiles of ozone within the atmospheric boundary layer, we developed and evaluated an uncrewed aircraft system (UAS) platform for measuring ozone and meteorological parameters of temperature, pressure, and humidity. To evaluate this approach, a UAS was flown with a portable ozone monitor and a meteorological temperature and humidity sensor to compare to tall tower measurements in northern Wisconsin. In June 2020, as a part of the WiscoDISCO20 campaign, a DJI M600 hexacopter UAS was flown with the same sensors to measure Lake Michigan shoreline ozone concentrations. This latter UAS experiment revealed a low-altitude structure in ozone concentrations in a shoreline environment showing the highest ozone at altitudes from 20–100 m a.g.l. These first such measurements of low-altitude ozone via a UAS in the Great Lakes region revealed a very shallow layer of ozone-rich air lying above the surface.

Meteorology & Atmospheric Sciences↗

TRACER SPARC AERI Data

These files contain 24 hour periods of retrieved thermodynamic profiles derived from observations collected by the University of Wisconsin–Madison Space Science and Engineering Center Portable Atmospheric Research Center (SPARC) Atmospheric Emitted Radiance Interferometer (AERI). The TROPoe algorithm is a python equivalent to the AERIoe algorithm (see Turner and Loehnert, 2014; Turner and Blumberg 2019). These data were collected during the TRACER project.

54 ENVIRONMENTAL SCIENCES↗

bioreactor sludge metagenome

Anaerobic fermentation bioreactor microbial communities from Madison, Wisconsin, United States. The goal of this study is to characterize a central set of genes relevant to medium chain fatty acid (MCFA) production to guide screens of putative MCFA-producing microorganisms from additional environments or grown on different substrates.

butyric↗

Measurements of the earth's airglow in the vacuum ultraviolet

A valuable by-product of the OAO-2 astronomy mission has been the first extensive set of measurements of the earth's airglow between 1000 and 3000 A. These measurements, made with the Wisconsin experiment package, provide clues to the structure and chemistry of the upper atmosphere. The most significant results from these observations are: (1) the detailed altitude profile of the emissions from the dark and sunlit earth limb; and (2) the confirmation of recent theories concerning the source of the dayglow radiation between 1350 and 1700 A.

Waters, C. R.↗

The temporal characteristics of the terrestrial radiation belt

The terrestrial radiation belt effects on the detectors in the Wisconsin equipment are considered. The lowest dark count rate observed in orbit, compared with that measured in the thermal vacuum test on the ground, shows no measurable difference for the solar blind ASCOP 541-F photomultiplier tubes while the EMI-6256B tubes show an increase in minimum dark count of a factor of from 20 to 150 times that observed on the ground. All instruments are perturbed when in the South Atlantic anomaly, but the EMI tubes are by far the most sensitive to it. Indeed, the effect never goes away.

Mcnall, J. F.↗

Observations of Venus, Mars, Jupiter and Saturn longward of 2000 A

A number of high quality spectra of Mars, Jupiter, the combined disk and rings of Saturn, as well as lower quality spectra of Venus, have been obtained with an objective grating spectrometer of the Wisconsin experiment package on OAO-2. An intercomparison of the Mars, Jupiter and Saturn spectra has failed to disclose any discrete planetary absorptions. Continuous albedos have been derived for the four planets by using OAO-2 observations of G-type stars for wavelengths greater than 2700 A and a new photoelectric solar spectrum for wavelengths smaller than 2700 A. The geometric albedo of Mars shows a steady increase below 3500 A which appears to be due to Rayleigh scattering combined with a decreasing surface reflectivity. The albedos of Venus, Jupiter and Saturn show a maximum in reflectivity in the region of 2500 A.

Wallace, L.↗

Interstellar extinction in the ultraviolet

Interstellar extinction curves over the region 3600-1100 A for 17 stars are presented. The observations were made by the two Wisconsin spectrometers onboard the OAO-2 with spectral resolutions of 10 A and 20 A. The extinction curves generally show a pronounced maximum at 2175 plus or minus 25 A, a broad minimum in the region 1800-1350 A, and finally a rapid rise to the far ultraviolet. Large extinction variations from star to star are found, especially in the far ultraviolet; however, with only two possible exceptions in this sample, the wavelength at the maximum of the extinction bump is essentially constant. These data are combined with visual and infrared observations to display the extinction behavior over a range in wavelength of about a factor of 20.

Bless, R. C.↗

Ultraviolet photometry of the eclipsing variable CW Cephei

An extended series of photometric observations were made of the eclipsing variable CW Cephei using the Wisconsin instrument on OAO-2. Approximate elements which were derived based solely on the eclipse depths and shape of the secondary are in satisfactory agreement with those found using ground based observations. However, persistent asymmetries and anomalous light variations, all larger than the expected experimental error, were also found; subsequent ground-based observations show H sub alpha entirely in emission indicating the presence of an extended gaseous system surrounding one or both of the components. Consistent solutions utilizing all data at all wavelengths were not found.

Sobieski, S.↗

Ultraviolet photometry of planetary nebulae

Nine of the planetary nebulae observed by the Wisconsin filter photometers are compared with 15 Monocerotis in the spectral region 1430-4250 A. The data are corrected for the degradation of the filters of stellar photometer number four with time. Comparisons with simple models indicate that most of the observed nebulae are subject to some interstellar extinction in the far ultraviolet. However, NGC 246 and NGC 1360 appear to be nearly unreddened. Thus far no unexpected features have been found in the observations.

Holm, A. V.↗

A survey of local interstellar hydrogen from OAO-2 observations of Lyman alpha absorption

The Wisconsin far ultraviolet spectrometer aboard OAO-2 observed the wavelength region near 1216 A for 69 stars of spectral type B2 or earlier. From the strength of the observed interstellar L sub alpha absorption, atomic hydrogen column densities were derived over distances averaging 300 pc away from the sun. The OAO data were compared to synthetic ultraviolet spectra, originally derived from earlier higher resolution rocket observations, which were computer processed to simulate the effects of absorption by different amounts of hydrogen followed by the instrumental blending.

Savage, B. D.↗

The optical properties of platinum and gold in the vacuum ultraviolet

The optical constants of platinum and gold thin films have been determined in the spectral region of 40 to 200 nm by reflection measurements. The highly polarized continuum of synchrotron radiation emitted by the 240-MeV electron storage ring at the Physical Sciences Laboratory of the University of Wisconsin was used as a light source for the spectrum below 120 nm, while a windowless discharge lamp coupled to a normal incidence monochromator provided a source for the longer wavelengths. Optical constants were determined by a computer program based on iterative solutions to the Fresnel equations for reflection as a function of the angle of incidence.

Linton, R. C.↗