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This paper presents an initial assessment of lost command and control (LC2L) procedures for large Uncrewed Aircraft Systems (UAS) in a simulated representative airspace environment using real airspace procedures. The experiment matrix consists of nine different flight routes: four nominal, four following current LC2L procedures, and one that routes the UAS more conservatively through less-busy airspace. For each route, eleven simulated UAS flights – in ten-minute increments – were flown into Fort Worth Alliance Airport following a real Instrument Approach Procedure. The simulated UAS flew amongst real recorded tracks of approximately 4,700 flights on January 18, 2022. The analysis focused primarily on the number of aircraft with which each UAS lost separation and where the losses occurred. This work presents a significant increase in testing capability and provides the foundation for further verification and validation of LC2L procedures using additional analysis metrics.