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At least 307 records · Page 17

Two-Photon Flow Cytometry

Flow cytometry is a powerful technique for obtaining quantitative information from fluorescence in cells. Quantitation is achieved by assuring a high degree of uniformity in the optical excitation and detection, generally by using a highly controlled flow such as is obtained via hydrodynamic focusing. In this work, we demonstrate a two-beam, two- channel detection and two-photon excitation flow cytometry (T(sup 3)FC) system that enables multi-dye analysis to be performed very simply, with greatly relaxed requirements on the fluid flow. Two-photon excitation using a femtosecond near-infrared (NIR) laser has the advantages that it enables simultaneous excitation of multiple dyes and achieves very high signal-to-noise ratio through simplified filtering and fluorescence background reduction. By matching the excitation volume to the size of a cell, single-cell detection is ensured. Labeling of cells by targeted nanoparticles with multiple fluorophores enables normalization of the fluorescence signal and thus ratiometric measurements under nonuniform excitation. Quantitative size measurements can also be done even under conditions of nonuniform flow via a two-beam layout. This innovative detection scheme not only considerably simplifies the fluid flow system and the excitation and collection optics, it opens the way to quantitative cytometry in simple and compact microfluidics systems, or in vivo. Real-time detection of fluorescent microbeads in the vasculature of mouse ear demonstrates the ability to do flow cytometry in vivo. The conditions required to perform quantitative in vivo cytometry on labeled cells will be presented.

Zhog, Cheng Frank↗

Performance of A Real-Time Photon Counting Optical Receiver in the Presence of Emulated Channel Fading

Free-space optical communication links with terrestrial ground stations experience fading due to atmospheric scintillation and beam pointing. Fiber-coupled receiver systems experience additional fading at the interface between the fiber and free-space optics of the telescope. The National Aeronautics and Space Administration (NASA) Glenn Research Center (GRC) has characterized a real-time photon-counting optical ground receiver system with an atmospheric fade emulation system. The receiver system is comprised of a fiber interconnect, an array of superconducting nanowire single photon detectors (SNSPDs), and a field programmable gate array (FPGA) based receive modem. Two fiber interconnect/detector architectures have been studied. One architecture uses a 70-mode photonic lantern coupled to seven single pixel SNSPDs. The other architecture uses a 10-mode few-mode fiber (FMF) coupled to a 15-pixel SNSPD array. The receiver system complies with the Consultative Committee for Space Data Systems (CCSDS) Optical Communications High Photon Efficiency Coding and Synchronization Standard, which uses serially concatenated convolutionally coded pulse-position modulation (SCPPM). The CCSDS standard is designed for use in low photon flux missions, including the Orion Artemis-II Optical (O2O) communications demonstration. The standard utilizes a convolutional symbol interleaver which can be resized to mitigate different fades. The fade emulation system employed in this work emulates scintillation-induced, pointing-induced, and coupling-induced fading. This paper gives an overview of the real-time optical receiver system and the fade emulation system. It presents tests results which show the impact of fading on the performance on the receiver. The test results show that in the presence of channel fading, the 70-mode photonic lantern outperforms the 10-mode FMF under higher (D/r_0=9) turbulence conditions due to high fiber-coupling-induced fading and fiber coupling loss on the 10-mode FMF. When operating in lower turbulence (D/r_0=4), the 10-mode FMF outperforms the 70-mode photonic lantern. The paper also shows a larger convolutional interleaver improves the system performance as long as the receiver does not lose acquisition.

optical communications↗

Performance of a real-time photon counting optical receiver in the presence of emulated channel fading

Free-space optical communication links with terrestrial ground stations experience fading due to atmospheric scintillation and beam pointing. Fiber-coupled receiver systems experience additional fading at the interface between the fiber and free-space optics of the telescope. The National Aeronautics and Space Administration (NASA) Glenn Research Center (GRC) has characterized a real-time photon-counting optical ground receiver system with an atmospheric fade emulation system. The receiver system is comprised of a fiber interconnect, an array of superconducting nanowire single photon detectors (SNSPDs), and a field programmable gate array (FPGA) based receive modem. Two fiber interconnect/detector architectures have been studied. One architecture uses a 70-mode photonic lantern coupled to seven single pixel SNSPDs. The other architecture uses a 10-mode few-mode fiber (FMF) coupled to a 15-pixel SNSPD array. The receiver system complies with the Consultative Committee for Space Data Systems (CCSDS) Optical Communications High Photon Efficiency Coding and Synchronization Standard, which uses serially concatenated convolutionally coded pulse-position modulation (SCPPM). The CCSDS standard is designed for use in low photon flux missions, including the Orion Artemis-II Optical (O2O) communications demonstration. The standard utilizes a convolutional symbol interleaver which can be resized to mitigate different fades. The fade emulation system employed in this work emulates scintillation-induced, pointing-induced, and coupling-induced fading. This paper gives an overview of the real-time optical receiver system and the fade emulation system. It presents tests results which show the impact of fading on the performance on the receiver. The test results show that in the presence of channel fading, the 70-mode photonic lantern outperforms the 10-mode FMF under higher (D/r_0=9) turbulence conditions due to high fiber-coupling-induced fading and fiber coupling loss on the 10-mode FMF. When operating in lower turbulence (D/r_0=4), the 10-mode FMF outperforms the 70-mode photonic lantern. The paper also shows a larger convolutional interleaver improves the system performance as long as the receiver does not lose acquisition.

optical communications↗

Numerical Solution of the Radiative Transfer Equation: X-Ray Spectral Formation from Cylindrical Accretion onto a Magnetized Neutron Star

Predicting the emerging X-ray spectra in several astrophysical objects is of great importance, in particular when the observational data are compared with theoretical models. This requires developing numerical routines for the solution of the radiative transfer equation according to the expected physical conditions of the systems under study. Aims. We have developed an algorithm solving the radiative transfer equation in the Fokker-Planck approximation when both thermal and bulk Comptonization take place. The algorithm is essentially a relaxation method, where stable solutions are obtained when the system has reached its steady-state equilibrium. Methods. We obtained the solution of the radiative transfer equation in the two-dimensional domain defined by the photon energy E and optical depth of the system pi using finite-differences for the partial derivatives, and imposing specific boundary conditions for the solutions. We treated the case of cylindrical accretion onto a magnetized neutron star. Results. We considered a blackbody seed spectrum of photons with exponential distribution across the accretion column and for an accretion where the velocity reaches its maximum at the stellar surface and at the top of the accretion column, respectively. In both cases higher values of the electron temperature and of the optical depth pi produce flatter and harder spectra. Other parameters contributing to the spectral formation are the steepness of the vertical velocity profile, the albedo at the star surface, and the radius of the accretion column. The latter parameter modifies the emerging spectra in a specular way for the two assumed accretion profiles. Conclusions. The algorithm has been implemented in the XPEC package for X-ray fitting and is specifically dedicated to the physical framework of accretion at the polar cap of a neutron star with a high magnetic field (approx > 10(exp 12) G). This latter case is expected to be of typical accreting systems such as X-ray pulsars and supergiant fast X ray transients.

Fairnelli, R.↗

Doppler lidar atmospheric wind sensors - A comparative performance evaluation for global measurement applications from earth orbit

A comparison is made of four prominent Doppler lidar systems, ranging in wavelength from the near UV to the middle IR, which are presently being studied for their potential in an earth-orbiting global tropospheric wind field measurement application. The comparison is restricted to relative photon efficiencies, i.e., the required number of transmitted photons per pulse is calculated for each system for midtropospheric velocity estimate uncertainties ranging from + or - 1 to + or - 4 m/s. The results are converted to laser transmitter pulse energy and power requirements. The analysis indicates that a coherent CO2 Doppler lidar operating at 9.11-micron wavelength is the most efficient.

Menzies, R. T.↗

High-Rate Data-Capture for an Airborne Lidar System

A high-rate data system was required to capture the data for an airborne lidar system. A data system was developed that achieved up to 22 million (64-bit) events per second sustained data rate (1408 million bits per second), as well as short bursts (less than 4 s) at higher rates. All hardware used for the system was off the shelf, but carefully selected to achieve these rates. The system was used to capture laser fire, single-photon detection, and GPS data for the Slope Imaging Multi-polarization Photo-counting Lidar (SIMPL). However, the system has applications for other laser altimeter systems (waveform-recording), mass spectroscopy, xray radiometry imaging, high-background- rate ranging lidar, and other similar areas where very high-speed data capture is needed. The data capture software was used for the SIMPL instrument that employs a micropulse, single-photon ranging measurement approach and has 16 data channels. The detected single photons are from two sources those reflected from the target and solar background photons. The instrument is non-gated, so background photons are acquired for a range window of 13 km and can comprise many times the number of target photons. The highest background rate occurs when the atmosphere is clear, the Sun is high, and the target is a highly reflective surface such as snow. Under these conditions, the total data rate for the 16 channels combined is expected to be approximately 22 million events per second. For each photon detection event, the data capture software reads the relative time of receipt, with respect to a one-per-second absolute time pulse from a GPS receiver, from an event timer card with 0.1-ns precision, and records that information to a RAID (Redundant Array of Independent Disks) storage device. The relative time of laser pulse firings must also be read and recorded with the same precision. Each of the four event timer cards handles the throughput from four of the channels. For each detection event, a flag is recorded that indicates the source channel. To accommodate the expected maximum count rate and also handle the other extreme of very low rates occurring during nighttime operations, the software requests a set amount of data from each of the event timer cards and buffers the data. The software notes if any of the cards did not return all the data requested and then accommodates that lower rate. The data is buffered to minimize the I/O overhead of writing the data to storage. Care was taken to optimize the reads from the cards, the speed of the I/O bus, and RAID configuration.

Valett, Susan↗

A note on the calculation of photon loss in an image-tube scanner.

It is found that in the case of 500 image elements and system gains above 3000 only a few per cent of input photons will be lost. Photon loss is not excessive for even lower gains. Aperture variations within a fairly wide range have little effect on the loss. However, losses increase if dead time at the end of a sweep is permitted. A system with continuous sweep at the fastest possible rates is recommended for optimal results.

Mcnall, J. F.↗

The High Resolution Imaging Instrument for HEAO-B

The High Resolution Imaging Instrument for the HEAO-B X-ray Telescope satellite is designed to reproduce images of celestial X-ray sources in the range of 0.2 to 4.0 keV. It provides both imaging and temporal data. The basic detector is a two-stage microchannel plate with a crossed-grid charge detector. Preamplifiers, detector bias supplies, and some processing circuits are colocated with the detector. The remaining electronic circuits are located in a remote central electronic assembly. The processing system is designed for single-photon processing. The imaging data rate is limited to 100 data points per second. Non-imaging total-field count rate is acquired with a maximum dead time of 14 microseconds per event.

Kubierschky, K.↗

Simulation studies for a nuclear photon pumped excimer laser

Simulation studies were undertaken to determine the feasibility of a nuclear photon pumped excimer laser using a system where high pressure Xe is bombarded with electrons and protons to form 1720 A. Primary measurements included conversion efficiency and gain vs time measurements.

Miller, T. G.↗

A position sensitive detector for EUV remote sensing

The authors describe a photon-counting extreme ultraviolet (EUV) detector system used in a rocket-borne spectroscopic instrument for remote sensing of upper atmospheric composition and temperature. The detector uses a KBr coated microchannel plate (MCP) Z stack in combination with a wedge-and-strip image readout system. Three separate detector fields of view are used to sense the Earth dayglow spectrum (980 A to 1040 A, and 1300 A to 1360 A) and the solar EUV spectrum (250 A to 1400 A). The authors demonstrate high gain (2 x 107), tight pulse-height distribution (35 percent FWHM), and a spatial resolution of about 35 microns FWHM (full width at half maximum), which is the highest resolution for a wedge-and-strip anode MCP detector flown to date. The background, image linearity, and flat-field performance are discussed. Raw spectra from the rocket flight are also presented.

Siegmund, O. H. W.↗

R and T report: Goddard Space Flight Center

The 1993 Research and Technology Report for Goddard Space Flight Center is presented. Research covered areas such as (1) flight projects; (2) space sciences including cosmology, high energy, stars and galaxies, and the solar system; (3) earth sciences including process modeling, hydrology/cryology, atmospheres, biosphere, and solid earth; (4) networks, planning, and information systems including support for mission operations, data distribution, advanced software and systems engineering, and planning/scheduling; and (5) engineering and materials including spacecraft systems, material and testing, optics and photonics and robotics.

Soffen, Gerald A.↗

Advanced Interconnect Roadmap for Space Applications

This paper presents the NASA electronic parts and packaging program for space applications. The topics include: 1) Forecasts; 2) Technology Challenges; 3) Research Directions; 4) Research Directions for Chip on Board (COB); 5) Research Directions for HDPs: Multichip Modules (MCMs); 6) Research Directions for Microelectromechanical systems (MEMS); 7) Research Directions for Photonics; and 8) Research Directions for Materials. This paper is presented in viewgraph form.

Galbraith, Lissa↗

Water stress, CO2 and photoperiod influence hormone levels in wheat

'Super Dwarf' wheat (Triticum aestivum L.) plants have been grown from seed to maturity in the Mir space station where they were periodically exposed, because of microgravity and other constraints, to water deficit, waterlogging, high CO2 levels, and low light intensities. The plants produced many tillers, but none of them produced viable seed. Studies have been initiated to determine why the plants responded in these ways. In the present study, effects of the listed stresses on abscisic acid (ABA), indole-3-acetic acid (IAA) and isopentenyl adenosine ([9R]iP) levels in roots and leaves of plants grown under otherwise near optimal conditions on earth were measured. Hormones were extracted, purified by HPLC, and quantified by noncompetitive indirect ELISA. In response to water deficit, ABA levels increased in roots and leaves, IAA levels decreased in roots and leaves, and [9R]iP levels increased in leaves but decreased in roots. In response to waterlogging, ABA, IAA and [9R]iP levels briefly increased in roots and leaves and then decreased. When portions of the root system were exposed to waterlogging and/or water deficit, ABA levels in leaves increased while [9R]iP and IAA levels decreased. These responses were correlated with the percentage of the root system stressed. At a low photosynthetic photon flux (100 micromoles m-2 s-1), plants grown in continuous light had higher leaf ABA levels than plants grown using an 18 or 21 h photoperiod.

Non-NASA Center↗

Proposed Functional Description for Phased Arrays

Generally speaking, many photonic engineers, while working in a systems development mode, still focus on presenting the unique physical details of the optical elements, instead of using functional representation to describe the system. The purpose of this presentation is to introduce symbols that can be used to represent the functional intent of most of the phased array architecture.

Phased Arrays systems development mode optical ele↗

Radiolysis and Photolysis of Icy Satellite Surfaces: Experiments and Theory

The transport and exchange of material between bodies in the outer solar system is often facilitated by their exposure to ionizing radiation. With this in mind we review the effects of energetic ions, electrons and UV photons on materials present in the outer solar system. We consider radiolysis, photolysis, and sputtering of low temperature solids. Radiolysis and photolysis are the chemistry that follows the bond breaking and ionization produced by incident radiation, producing, e.g., O2 and H2 from irradiated H2O ice. Sputtering is the ejection of molecules by incident radiation. Both processes are particularly effective on ices in the outer solar system. Materials reviewed include H2O ice, sulfur-containing compounds (such as S02 and S8), carbon~contajning compounds (such as CH4), nitrogen-containing compounds (such as NH3 and N2), and mixtures of those compounds. We also review the effects of ionizing radiation on a mixture of N2 and CH4 gases, as appropriate to Titan's upper atmosphere, where radiolysis and photolysis produce complex organic compounds (tholins).

Cassidy, T.↗

Miniaturized, High-Speed, Modulated X-Ray Source

A low-cost, miniature x-ray source has been developed that can be modulated in intensity from completely off to full intensity on nanosecond timescales. This modulated x-ray source (MXS) has no filaments and is extremely rugged. The energy level of the MXS is adjustable from 0 to more than 100 keV. It can be used as the core of many new devices, providing the first practical, arbitrarily time-variable source of x-rays. The high-speed switching capability and miniature size make possible many new technologies including x-ray-based communication, compact time-resolved x-ray diffraction, novel x-ray fluorescence instruments, and low- and precise-dose medical x-rays. To make x-rays, the usual method is to accelerate electrons into a target material held at a high potential. When the electrons stop in the target, x-rays are produced with a spectrum that is a function of the target material and the energy to which the electrons are accelerated. Most commonly, the electrons come from a hot filament. In the MXS, the electrons start off as optically driven photoelectrons. The modulation of the x-rays is then tied to the modulation of the light that drives the photoelectron source. Much of the recent development has consisted of creating a photoelectrically-driven electron source that is robust, low in cost, and offers high intensity. For robustness, metal photocathodes were adopted, including aluminum and magnesium. Ultraviolet light from 255- to 350-nm LEDs (light emitting diodes) stimulated the photoemissions from these photocathodes with an efficiency that is maximized at the low-wavelength end (255 nm) to a value of roughly 10(exp -4). The MXS units now have much higher brightness, are much smaller, and are made using a number of commercially available components, making them extremely inexpensive. In the latest MXS design, UV efficiency is addressed by using a high-gain electron multiplier. The photocathode is vapor-deposited onto the input cone of a Burle Magnum(TradeMark) multiplier. This system yields an extremely robust photon-driven electron source that can tolerate long, weeks or more, exposure to air with negligible degradation. The package is also small. When combined with the electron target, necessary vacuum fittings, and supporting components (but not including LED electronics or high-voltage sources), the entire modulated x-ray source weighs as little as 158 grams.

Gendreau, Keith↗

Lenslet Array to Further Suppress Star Light for Direct Exoplanet Detection

Direct imaging plays a key role in the detection and characterization of exoplanets orbiting within its host star's habitable zone. Many innovative ideas for starlight suppression and wavefront control have been proposed and developed over the past decade. However, several technological challenges still lie ahead to achieve the required contrast, including controlling the observatory pointing performance, fabricating occulting masks with tight optical tolerances, developing wavefront control algorithms, controlling stray light, advancing single photon detecting detectors, and integrated system-level issues. This paper explores how a lenslet array and pinhole mask may be implemented to further suppress uncorrected starlight that leaks through the occulting mask. An external occulter, or star shade, is simulated to demonstrate this concept, although this approach can be implemented for internal coronagraphs as well. We describe how to use simple relay optics to control the scene near the inner working angle and the level of the suppression expected. Furthermore, if the lenslet array is the input to an integral field spectrograph, as planned for the WFIRST mission, the spectral content of the exoplanet atmospheres can be obtained to determine if the observed planet is habitable and ultimately, if it is inhabited.

Integral Field spectrograph↗

Surface Height and Sea Ice Freeboard of the Arctic Ocean from ICESat-2: Characteristics and Early Results

We present the first winter season of surface height and sea ice freeboards of the Arctic Ocean from the new ICESat-2 (IS-2) mission. The Advanced Topographic Laser Altimeter System (ATLAS) onboard has six photon-counting beams for surface profiling with a 10-kHz pulse-rate (inter-pulse distance ~0.7m) and footprints of ~17m. Geolocated heights assigned to individual photons scattered from the surface allow significant flexibility in the construction of height distributions used in surface finding. For IS-2 sea ice products, a fixed 150-photon aggregate is used to control height precision and obtain better along-track resolution over high reflectance surfaces. Quasi-specular returns in openings as narrow as ~27m, crucial for freeboard calculations, are resolved. The fixed photon aggregate results in unique variable along-track resolutions and non-uniform sampling (17m x 27m to 17m x 200m for the strong beams) of the surface. The six profiling beams – three pairs separated by 3.3 km with a strong and weak beam in each pair – provide correlated statistics at regional length scales for assessment of beam-to-beam retrieval consistency and accuracy. Analysis shows along-track height precisions of ~2cm, and agreement in the monthly freeboard distributions across the strong beams to 1-2cm. In this paper, we describe briefly the approaches used in surface height and freeboard retrievals from ATLAS photon clouds, and detail the key features of these along-track sea ice products, focusing on the first release of data collected over the Arctic Ocean, which spans the period between October 14, 2018 – the start of data collection – and the end of March 2019.

R Kwok↗