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At least 343 records · Page 19

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NASA NTRS1990

A porous stainless steel membrane system for extraterrestrial crop production

A system was developed in which nutrient flow to plant roots is controlled by a thin (0.98 or 1.18 mm) porous (0.2 or 0.5 microns) stainless steel sheet membrane. The flow of nutrient solution through the membrane is controlled by adjusting the relative negative pressure on the nutrient solution side of the membrane. Thus, the nutrient solution is contained by the membrane and cannot escape from the compartment even under microgravity conditions if the appropriate pressure gradient across the membrane is maintained. Plant roots grow directly on the top surface of the membrane and pull the nutrient solution through this membrane interface. The volume of nutrient solution required by this system for plant growth is relatively small, since the plenum, which contains the nutrient solution in contact with the membrane, needs only to be of sufficient size to provide for uniform flow to all parts of the membrane. Solution not passing through the membrane to the root zone is recirculated through a reservoir where pH and nutrient levels are controlled. The size of the solution reservoir depends on the sophistication of the replenishment system. The roots on the surface of the membrane are covered with a polyethylene film (white on top, black on bottom) to maintain a high relative humidity and also limit light to prevent algal growth. Seeds are sown directly on the stainless steel membrane under the holes in the polyethylene film that allow a pathway for the shoots.

NASA Discipline Number 61-20↗

NASA NTRS2006

Making Plant-Support Structures From Waste Plant Fiber

Environmentally benign, biodegradable structures for supporting growing plants can be made in a process based on recycling of such waste plant fiber materials as wheat straw or of such derivative materials as paper and cardboard. Examples of structures that can be made in this way include plant plugs, pots, planter-lining mats, plant fences, and root and shoot barriers. No chemical binders are used in the process. First, the plant material is chopped into smaller particles. The particles are leached with water or steam to remove material that can inhibit plant growth, yielding a fibrous slurry. If the desired structures are plugs or sheets, then the slurry is formed into the desired shapes in a pulp molding subprocess. If the desired structures are root and shoot barriers, pots, or fences, then the slurry is compression-molded to the desired shapes in a heated press. The processed materials in these structures have properties similar to those of commercial pressboard, but unlike pressboard, these materials contain no additives. These structures have been found to withstand one growth cycle, even when wet

Morrow, Robert C.↗

NASA NTRS1994

Diurnal changes in net uptake rate of nitrate are associated with changes in estimated export of carbohydrates to roots

The rate of NO3- uptake by soybean (Glycine max [L.] Merrill) roots generally declines during the night in association with progressive depletion of the nonstructural carbohydrate pool in the shoot as well as the concentration of carbohydrates in roots. To determine if NO3- uptake rate changes in response to variations in translocation rate of carbohydrates from shoot to roots per se or to carbohydrate status of the roots, the night period was interrupted with a low light level from incandescent lamps to alter the diurnal pattern of NO3- uptake by roots and export of carbohydrate from shoots of nonnodulated soybean. Depletion of NO3- from replenished, complete nutrient solutions containing 1 mM NO3- was measured by ion chromatography and rates of NO3- uptake were calculated. Changes in export of carbohydrates from shoot to roots during intervals of the night period were calculated as the differences between rates of disappearance in contents of nonstructural carbohydrates and their estimated rates of utilization in shoot respiration and growth. A positive, significant correlation occurred between changes in calculated rates of carbohydrate export from shoots and NO3- uptake rates. Conversely, there was no significant correlation between concentrations of nonstructural carbohydrates in roots and NO3- uptake rates. These results support the hypothesis that carbohydrate flux from shoot to roots has a direct role in regulation of nitrogen uptake by the whole plant.

Non-NASA Center↗

NASA NTRS2019

Plant Water Management (PWM)

The NASA Plant Water Management technology demonstration exploits recent advances in microgravity capillary fluidics to develop techniques for plant growth in a space environment. Plant growth in the low-g environment is often hampered by inadequate aeration and over-saturation in the root zone. The present effort aims to make passive use of poorly wetting liquids (i.e., contaminated water) within unique system geometries that effectively replace the role of gravity in providing sufficient aeration and hydration for simulated plants. Several ISS demonstrations are in preparation for launch in 2019, including hydroponic and soil approaches. Supportive terrestrial and low-g drop tower tests are conducted to aid in experiment design via proof-of-concepts, limits of operation, system stability, and others. The implications to short and long term applications are discussed in relation to plant growth facilities for both near-term microgravity plant science research and long duration human exploration missions.

Hatch, Tyler↗

NASA NTRS1974

A survey for the use of remote sensing in the Chesapeake Bay region

Environmental problem areas concerning the Chesapeake Bay region are reviewed along with ongoing remote sensing programs pertaining to these problems, and recommendations are presented to help fill lacunae in present research and to utilize the remote sensing capabilities of NASA to their fullest. A list of interested organizations and individuals is presented for each category. The development of technologies to monitor dissolved nutrients in bay waters, the initiation of a census of the disappearing rooted acquatic plants in the littoral zones, and the mapping of natural building constraints in the growth regions of the states of Maryland and Virginia are among the recommendations presented.

Ulanowicz, R. E.↗

NASA NTRS1982

Enhancement of in vitro Guayule propagation

A method for stimulating in vitro propagation of Guayule from a nutrient medium containing Guayule tissue by adding a substituted trialkyl amine bioinducing agent to the nutrient medium is described. Selective or differentiated propagation of shoots or callus is obtained by varying the amounts of substituted trialky amine present in the nutrient medium. The luxuriant growth provided may be processed for its poly isoprene content or may be transferred to a rooting medium for production of whole plants as identical clones of the original tissue. The method also provides for the production of large numbers of Guayule plants having identical desirable properties such as high polyisoprene levels.

Dastoor, M. N.↗

NASA NTRS1988

Current-driven resistive ballooning modes in axially bounded solar flare plasmas

The most unstable current-driven resistive modes of an axially bounded coronal loop are found in computer simulations to exhibit the spatial structure of ballooning modes. The observed modes are not confined to mode rational surfaces, but instead have broad radial extent. A theory assuming ballooning mode spatial structure predicts that a minimum current should be required for linear instability, and that, when the mode is unstable, the linear growth rate scales linearly with the resistivity eta below a critical resistivity, and scales as cu root of eta for larger resistivities. Both predictions are borne out by simulation results. Both theory and simulation analyses of the mode suggest that the strong radial structure of the mode near the ends of the system is the primary contributing factor to the instability of the mode. A helical current sheet is formed in the nonlinear evolution of the mode near the edge of the current channel and is accompanied by a strong radial gradient in the current and partial current reversal.

Otani, Niels F.↗

NASA NTRS1985

Abscisic acid, xanthoxin and violaxanthin in the caps of gravistimulated maize roots

The occurrence and distribution of abscisic acid (ABA), xanthoxin (Xa) and the carotenoid violaxanthin (Va) were investigated in root tips of maize (Zea mays L. cv. Merit). In roots grown in the dark, Va and ABA were present in relatively high amounts in the root cap and in low amounts in the adjacent terminal 1.5 mm of the root. Xanthoxin was present in equal concentrations in both regions. In roots exposed to light, the ABA distribution was reversed, with relatively low levels in the root cap and high levels in the adjacent 1.5-mm segment. Light also caused a decrease in Va in both regions of the root and an increase in Xa, especially in the cap. In the maize cultivar used for this work, light is necessary for gravitropic curving. This response occurs within the same time frame as the light-induced ABA redistribution as well as the changes in the levels of Va and Xa. These data are consistent with a role for ABA in root gravitropism and support the proposal that Xa may arise from the turnover of Va.

NASA Discipline Plant Biology↗

NASA NTRS2014

Veggie Hardware Validation Test Preliminary Results and Lessons Learned

The Veggie hardware validation test, VEG-01, was conducted on the International Space Station during Expeditions 39 and 40 from May through June of 2014. The Veggie hardware and the VEG-01 experiment payload were launched to station aboard the SpaceX-3 resupply mission in April, 2014. Veggie was installed in an Expedite-the-Processing-of-Experiments-to-Space-Station (ExPRESS) rack in the Columbus module, and the VEG-01 validation test was initiated. Veggie installation was successful, and power was supplied to the unit. The hardware was programmed and the root mat reservoir and plant pillows were installed without issue. As expected, a small amount of growth media was observed in the sealed bags which enclosed the plant pillows when they were destowed. Astronaut Steve Swanson used the wet/dry vacuum to clean up the escaped particles. Water insertion or priming the first plant pillow was unsuccessful as an issue prevented water movement through the quick disconnect. All subsequent pillows were successfully primed, and the initial pillow was replaced with a backup pillow and successfully primed. Six pillows were primed, but only five pillows had plants which germinated. After about a week and a half it was observed that plants were not growing well and that pillow wicks were dry. This indicated that the reservoir was not supplying sufficient water to the pillows via wicking, and so the team reverted to an operational fix which added water directly to the plant pillows. Direct watering of the pillows led to a recovery in several of the stressed plants; a couple of which did not recover. An important lesson learned involved Veggie's bellows. The bellows tended to float and interfere with operations when opened, so Steve secured them to the baseplate during plant tending operations. Due to the perceived intensity of the LED lights, the crew found it challenging to both work under the lights and read crew procedures on their computer. Although the lights are not a safety hazard, for visual comfort crewmembers were advised to wear sunglasses when working with the plants and then they can lift glasses to read procedures. Steve Swanson had already trail-blazed this procedure when he initiated VEG-01. The temperature and humidity data logger was relocated mid-experiment to provide measurements on both sides of the unit. Images of the plants were downlinked weekly, and videos of installation and harvest were recorded. This imaging frequency was not sufficient to monitor and respond to changes in plant growth. Plants, samples, and data loggers will be returned on SpaceX-4, scheduled to return the fall of 2014. Lessons learned will be translated into hardware and operational modifications for future Veggie payloads.

International Space Station↗

NASA NTRS1984

The growth of metastable peritectic compounds

The effects of directional solidification processing on the microstructural, compositional, and magnetic properties of high-melting-temperature, commercially important alloys which form from the liquid state via peritectic or eutectic type reactions were determined. Emphasis was placed on ferromagnetic compounds of the commercially important Co-Sm and Al-Mn systems. The primary dendrite spacing for eutectic Sm2Co17/Co scaled with negative square root of V and varied from approximately 50 microns for V 20 cm/h to hundreds of microns for V 10 cm/h. Since the crystal growth mechanism was dendritic rather than cooperative, the assoicated permanent magnet properties were rather poor. Magnetization as a function of sample orientation indicates that the easy axis of magnetization was primarily along the direction of solidification for the eutectic Sm2Co17/Co and peritectic SmCo5/Sm2Co17 compositions. For the Al-Mn case, magnetization and microstructural characterization suggest isotropic, polycrystalling growth for all solidification velocities studied.

Pirich, R. G.↗

NASA NTRS2018

Larch (Larix dahurica Turcz) growth response to climate change in the Siberian permafrost zone

Larch-dominant communities are the most extensive high-latitude forests in Eurasia and are experiencing the strongest impacts from warming temperatures. We analyzed larch (Larix dahurica Turcz) growth index (GI) response to climate change. The studied larch-dominant communities are located within the permafrost zone of Northern Siberia at the northern tree limit (ca. N 67° 38′, E 99° 07′). Methods included dendrochronology, analysis of climate variables, root zone moisture content, and satellitederived gross (GPP) and net (NPP) primary productivity. It was found that larch response to warming included a period of increased annual growth increment (GI) (from the 1970s to ca. 1995) with a follow on GI decline. Increase in GI correlated with summer air temperature, whereas an observed decrease in GI was caused by water stress (vapor pressure deficit and drought increase). Water stress impact on larch growth in permafrost was not observed before the onset of warming (ca. 1970). Water limitation was also indicated by GI dependence on soil moisture stored during the previous year. Water stress was especially pronounced for stands growing on rocky soils with low water-holding capacity. GPP of larch communities showed an increasing trend, whereas NPP stagnated. A similar pattern of GI response to climate warming has also been observed for Larix sibirica Ledeb, Pinus sibirica Du Tour, and Abies sibirica Ledeb in the forests of southern Siberia. Thus, warming in northern Siberia permafrost zone resulted in an initial increase in larch growth from the 1970s to the mid-1990s. After that time, larch growth increment has decreased. Since ca. 1990, water stress at the beginning of the vegetative period became, along with air temperature, a main factor affecting larch growth within the permafrost zone.

Larix dahurica↗

NASA NTRS2011

Growth of Three Lettuce Cultivars in NASA's HDU PEM During the 2010 DRATS Test

NASA's 2010 Desert Research and Technology Studies (DRATS) of the VEGGIE Food Production System in the Habitat Demonstration Unit (HDU) Pressurized Excursion Module (PEM) was the first operational evaluation of salad crop production technology in a NASA analog test. Rooting media and slow release fertilizers were evaluated for three lettuce cultivars that had shown promise as candidates for a surface based food production system. These tests involved comparing growth, color and quality of the lettuce cultivars grown under VEGGIE LED array (Orbitec, Madison, WI) or Biomass Production System for Education ((BSEe), Orbitec, Madison, WI) compact fluorescent lamps using a gravity feed water delivery system. Mission relevant conditions of CO2, temperature and RH were maintained using controlled environment chambers (EGC, Chagrin Falls, OH). Growth data was obtained for the two red leaf lettuce cultivars, Outredgeous and Firecracker, and the green Bibb lettuce cultivar, Flandria. Growth and quality was evaluated using different concentrations (7.5 g/L and 15 g/L) of commercial slow release fertilizer (Osmocote Plus 15-9-12, Scotts, Maryville, OH) and Nutricote 18-6-8 (Florikan, Sarasota, FL) in either a peat/vermiculite media (sunshine LP5 MiX, Sungro, Bellview, WA) or calcined montmorillonite clay [(arcillite,)Turface Proleague, Profile LLC, Buffalo Grove, IL]. The commercial peat/vermiculite mix generally resulted in larger plants than those grown in arcillite. Increasing the concentration of Osmocote from 7.5 to 15 g/L increased the height, dry mass, and leaf area of lettuce cultivars. In contrast, there was a decrease in growth parameters when concentration of Nutricote was increased from 7.5 to 15 g/L. The best growth was obtained with the 7.5 g/L Nutricote using a commercial peat/vermiculite mixture. This media was used for field testing VEGGIE plant system in the 2010 DRAT test. The VEGGIE nutrient delivery system worked well, was able to be maintained by multiple operators with a minimum of training, and supported excellent lettuce growth for the duration of the 14-day test. The operational DRAT field testing in the HDU identified light quality issues related to morphology and pigment development that will need to be addressed through additional testing. Feedback from the crew, ground support personnel, and human factors leads was uniformly positive on the psychological value of having the crop production system in the pressurized excursion module. Data are being used to design a plant atrium with LED lighting to evaluate salad crop growth during NASA's 2011 DRATS test.

Stutte, Gary W.↗

NASA NTRS1983

Some results from studies on the effects of weightlessness on the growth of epiphytic orchids

Epidendrum orchids were placed in a Malakhit-2 micro-greenhouse aboard the Soyuz-36-Salyut-6 space station to test their growth under weightless conditions. Growth occurred but was less than in control plants left on Earth; cells were smaller and parenchymal development slowed in all tissues. Stems, roots, and leaves were smaller. The number of stomas on the leaves was about the same as in the controls, but, because of the smaller leaf size, there were more per unit area. A modeling experiment using a clinostat revealed a large decrease in gibberellin activity and auxin activity. It was assumed that weightlessness primarily affects gibberellin biosynthesis, inhibiting cell growth. Reestablishment of growth compound activity upon return of the plants to Earth was indicated by the fact that the orchids resumed growth thereafter.

Cherevchenko, T. M.↗

NASA NTRS2012

Preparation for the Proof of Concept Flight of the Veggie Plant Growth Chamber

Veggie is a small plant growth chamber designed and built by ORBITEC that will fly to the International Space Station on SpaceX-3, scheduled for the summer of 2013. Ultimately Veggie will be used for research, education and outreach, and crew recreation. We want to demonstrate the functionality of this hardware by testing a scenario that could allow the crew to grow and consume fresh vegetables. Veggie will be collapsed and transported flat in a cargo transfer bag, and deployed on orbit, where it will be installed in an EXPRESS rack. The chamber consists of three subsystems: an LED light cap, a transparent bellows, and a root mat reservoir assembly. The bellows and flexible support arms allow the distance between plants and light cap to be adjusted for different ages and types of plants. Researchers at Kennedy Space Center and ORBITEC have been working to develop the plant growth interfaces for the proof of concept flight. We have developed a rooting pillow, consisting of a small bag containing media, time release fertilizer, seeds, and a wicking surface to conduct water from the root mat reservoir. Prototype pillows have been tested and results have influenced the design of flight pillows, which will be modified for microgravity from flight-approved materials. Several studies have been conducted selecting species and comparing media types in analog systems. Water content seems to be the most important factor differentiating media types in these small growth volumes (100 mL). Media type also influenced microbial levels on plants. Since produce sanitizing agents are not currently approved for growing food crops on orbit, plants and media types having very low microbial levels are being selected. Lettuce, mizuna, and other salad greens typically have microbial counts less than 10(exp 4) colony forming units and thus are good candidates for spaceflight. As we approach flight verification testing, we will finalize species, media selection, harvesting, and microbial sampling procedures. Next steps include testing of Veggie flight and ground hardware and associated equipment. This research was funded by NASA.

Massa, Gioia↗

NASA NTRS2004

Kinetic Roughening and Energetics of Tetragonal Lysozyme Crystal Growth: A Preliminary Atomic Force Microscopy Investigation

We examined particulars of crystal growth from measurements obtained at both microscopic and molecular levels. The crystal growth measurements performed at the microscopic level are well characterized by a model that balances the flux of macromolecules towards the crystal surface with the flux of the crystal surface. Numerical evaluation of model with measurements of crystal growth, in time, provided accurate estimates for the average growth velocities. Growth velocities thus obtained were also interpreted using well-established phenomenological theories. Moreover, we find that microscopic measurements of growth velocity measurements obtained as a function of temperature best characterizes changes in crystal growth modes, when present. We also examined the possibility of detecting a change in crystal growth modes at the molecular level using atomic force microscopy, AFM. From preliminary AFM measurements performed at various supersaturations, we find that magnitude of surface height fluctuations, h(x), increases with supersaturation. Further examination of surface height fluctuations using methods established for fluctuation spectroscopy also enabled the discovery of the existence of a characteristic length, c, which may possibly determine the mode of crystal growth. Although the results are preliminary, we establish the non- critical divergence of 5 and the root-mean-square (rms) magnitude of height-height fluctuations as the kinetic roughening transition temperatures are approached. Moreover, we also examine approximate models for interpreting the non-critical behavior of both 6 and rms magnitude of height-height fluctuations, as the solution supersaturation is increased towards the kinetic roughening supersaturation.

Gorti, Sridhar↗

NASA NTRS2005

Physical and hydraulic properties of baked ceramic aggregates used for plant growth medium

Baked ceramic aggregates (fritted clay, arcillite) have been used for plant research both on the ground and in microgravity. Optimal control of water and air within the root zone in any gravity environment depends on physical and hydraulic properties of the aggregate, which were evaluated for 0.25-1-mm and 1-2-mm particle size distributions. The maximum bulk densities obtained by any packing technique were 0.68 and 0.64 g cm-3 for 0.25-1-mm and 1-2-mm particles, respectively. Wettable porosity obtained by infiltration with water was approximately 65%, substantially lower than total porosity of approximately 74%. Aggregate of both particle sizes exhibited a bimodal pore size distribution consisting of inter-aggregate macropores and intra-aggregate micropores, with the transition from macro- to microporosity beginning at volumetric water content of approximately 36% to 39%. For inter-aggregate water contents that support optimal plant growth there is 45% change in water content that occurs over a relatively small matric suction range of 0-20 cm H2O for 0.25-1-mm and 0 to -10 cm H2O for 1-2-mm aggregate. Hysteresis is substantial between draining and wetting aggregate, which results in as much as a approximately 10% to 20% difference in volumetric water content for a given matric potential. Hydraulic conductivity was approximately an order of magnitude higher for 1-2-mm than for 0.25-1-mm aggregate until significant drainage of the inter-aggregate pore space occurred. The large change in water content for a relatively small change in matric potential suggests that significant differences in water retention may be observed in microgravity as compared to earth.

NASA Center JSC↗

NASA NTRS1990

Nitrogen nutrition and temporal effects of enhanced carbon dioxide on soybean growth

Plants grown on porous media at elevated CO2 levels generally have low concentrations of tissue N and often appear to require increased levels of external N to maximize growth response. This study determines if soybean [Glycine max (L.) Merr. Ransom'] grown hydroponically at elevated CO2 requires increases in external NO3- concentrations beyond levels that are optimal at ambient CO2 to maintain tissue N concentrations and maximize the growth response. This study also investigates temporal influences of elevated CO2 on growth responses by soybean. Plants were grown vegetatively for 34 d in hydroponic culture at atmospheric CO2 concentrations of 400, 650, and 900 microliters L-1 and during the final 18 d at NO3- concentrations of 0.5, 1.0, 5.0 and 10.0 mM in the culture solution. At 650 and 900 microliters L-1 CO2, plants had maximum increases of 31 and 45% in dry weight during the experimental period. Plant growth at 900 microliters L-1 CO2 was stimulated earlier than at 650 microliters L-1. During the final 18 d of the experiment, the relative growth rates (RGR) of plants grown at elevated CO2 declined. Elevated CO2 caused increases in total N and total NO3(-)-N content and leaf area but not leaf number. Enhancing CO2 levels also caused a decrease in root:shoot ratios. Stomatal resistance increased by 2.1- and 2.8-fold for plants at the 650 and 900 microliters L-1 CO2, respectively. Nitrate level in the culture solutions had no effect on growth or on C:N ratios of tissues, nor did increases in CO2 levels cause a decrease in N concentration of plant tissues. Hence, increases in NO3- concentration of the hydroponic solution were not necessary to maintain the N status of the plants or to maximize the growth response to elevated CO2.

NASA Program CELSS↗

NASA NTRS1994

Hydroponic Feed With Suction

Placing nutrient solution under suction increases growth. Foam plug seals growing stem of plant, making it possible to maintain suction in nutrient liquid around roots. Jar wrapped in black tape to keep out light. Potential use in terrestrial applications in arid climates or in labor-intensive agricultural situations.

Cox, William M.↗