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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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33 records · Page 2
All sourcesNASA NTRSDOE OSTI
DOE OSTI2026

Amazonian fog harbors viable microbes

Fog formation over tropical forests remains poorly characterized, despite its potential role in bioaerosol dispersion and ecosystem processes. Here, we analyzed fog samples collected at the Amazon Tall Tower Observatory using flow cytometry and culture-based techniques to characterize viable microbial communities. Microbial cell concentrations varied over an order of magnitude across 13 fog events, reaching up to 8 × 104 cells per ml of fog water. Flow cytometry consistently detected metabolically active cells, while culturing and mass spectrometry-based identification yielded eight viable bacterial species and seven fungal taxa. The bacteria Serratia marcescens, Ralstonia pickettii and Sphingomonas paucimobilis exhibited seasonal variations in prevalence. The fungal species identified were primarily mesophilic saprophytes and endophytes, commonly associated with soil and plant surfaces. Our findings indicate that fog harbors viable microbes, including Serratia marcescens and Ralstonia pickettii, which may imply a relevance of fog for microbial dispersal, colonization and nutrient cycling in the Amazon rainforest.

Godoi, Ricardo H. (ORCID:0000000247744870)↗
DOE OSTI2025

Time-series RNA metabarcoding of the active Populus tremuloides root microbiome reveals hidden temporal dynamics and dormant core members

The rhizosphere is a critical interface between plant roots and soil, harboring diverse microbial communities that are essential to plant and ecosystem health. Although these communities exhibit stark temporal dynamics, their dormancy/activity transitions remain poorly understood. Such transitions may enable microbes to rapidly adjust functional contributions faster than community turnover alone would allow. Here, we used RNA metabarcoding to characterize the active fraction of microbial communities on the roots of quaking aspen (Populus tremuloides) in a time-series study across a natural environmental gradient. We explore cryptic temporal microbial community dynamics of rhizosphere communities at the ecosystem scale. The active rhizosphere bacterial and fungal communities were more temporally dynamic than total communities, while total communities exhibited a stronger response to site-specific conditions. Notably, some core microbiome members were often inactive, yielding a smaller “active core” subset. The fungal endophyte Hyaloscypha finlandica was the only microbe that was both present and active in all plots across all timepoints. Soil temperature strongly influenced both total and active community composition, with the fungal class Eurotiomycetes showing a temperature-dependent seasonal decline in abundance. Together, these results reveal that modulation of microbial activity levels is a key mechanism by which the plant root holobiont responds to environmental variation, and that even dominant symbionts may frequently persist in dormancy within the rhizosphere.

Community Structure and Diversity↗
DOE OSTI2025

Enrichment of root-associated Streptomyces strains in response to drought is driven by diverse functional traits and does not predict beneficial effects on plant growth

The genus Streptomyces has consistently been found enriched in drought-stressed plant root microbiomes, yet the ecological basis and functional variation underlying this enrichment at the strain and isolate level remain unclear. Using two 16S rRNA sequencing methods with different levels of taxonomic resolution, we confirmed drought-associated enrichment (DE) of Streptomyces in field-grown sorghum roots and identified five closely related but distinct amplicon sequence variants (ASVs) belonging to the genus with variable drought enrichment patterns. From a culture collection of sorghum root endophytes, we selected 12 Streptomyces isolates representing these ASVs for phenotypic and genomic characterization. Whole-genome sequencing revealed substantial variation in gene content, even among closely related isolates, and exometabolomic profiling showed distinct metabolic responses to media supplemented with drought- versus well-watered root tissue. Traits linked to drought survival, including osmotic stress tolerance, siderophore production, and carbon utilization, varied widely among isolates and were not phylogenetically conserved. Using a broader panel of 48 Streptomyces, we demonstrate that DE scores, determined through mono-association experiments in gnotobiotic sorghum systems, showed high variability and lacked correlation with plant growth promotion. Pangenome-wide association identified orthogroups involved in osmolyte transport (e.g., proP) and membrane biosynthesis (e.g., fabG) as positively associated with DE, though most associations lacked phylogenetic signal. Collectively, these results demonstrate that Streptomyces DE is not a conserved genus-level trait but is instead strain-specific and functionally heterogeneous. Furthermore, DE in the root microbiome was shown not to predict beneficial effects on plant growth. This work underscores the need to resolve functional traits at the strain level and highlights the complexity of microbe-host-environment interactions under abiotic stress.

Fonseca-Garcia, Citlali↗
NASA NTRS1983

Prochloron

A review is given of the research problems associated with Prochloron. Two questions are discussed in detail. Did prochlorophytes arise in independently off cyanophytes, or did one of these classes arise from the other? If eukaryotic chlorophytes originated by symbiogenesis, might their plastids have arisen from symbiotic, endophytic prochlorophyte-like ancestors?

Lewin, R. A.↗
NASA NTRS1996

Development of the gametophyte of the fern Schizaea pusilla

Schizaea pusilla is a pteridophyte with several unique developmental characteristics. In contrast to most other fern species, S. pusilla gametophytes remain filamentous throughout their development, and the gametophytes are associated with an endophytic fungus which appears to be mycorrhizal. In terms of tropistic responses, apical filament cells of young gametophytes are negatively phototropic compared with germ filaments of other ferns which exhibit positive phototropism. Cryofixation (propane jet freezing and high-pressure freezing) in conjunction with freeze substitution electron microscopy was used to study young gametophytes. The results demonstrate that apical filament cells have a distinctive structural polarity and that rhizoids also can be successfully frozen by these methods. The cytoskeleton and endomembrane system were particularly well preserved in cryofixed cells. In addition, Schizaea gametophytes were used as a test system to evaluate potential artifacts of propane jet freezing and high pressure freezing. There was little apparent difference in ultrastructure between cells cryofixed by either freezing method. These gametophytes will be useful in determining the effectiveness of cryofixation techniques and as a model system in tip growth studies.

Non-NASA Center↗
NASA NTRS2010

Mutualism in a Reduced Gravity Environment (MuRGE)

Mutualism in a Reduced Gravity Environment (MuRGE) is a ground research study to determine the feasibility of assessing fungi-plant (Piriformospora indica-Arabidopsis thaliana) interactions in microgravity. Seeds from the plant Arabiddospsis thaliana (At) will be grown in the presence of Piriformospora indica (Pi) an endophytic Sebacinacae family fungus. Pi is capable of colonizing the roots of a wide variety of plant species, including non-mycorrhizal hosts like At, and promoting plant growth similarly to AMF (arbusuclar mychorrizal fungi) unlike most AMF, Pi is not an obligate plant symbiont and can be grown in the absence of a host. In the presence of a suitable plant host, Pi can attach to and colonize root tips. Interaction visualization is accomplished with strong autofluorescence in the roots, followed by root colonization via fungal hyphae, and chlamydospore production. Increased root growth can be observed even before root colonization is detectable. In addition, Pi chlamydospores generated from axenic culture in microgravity will be used to inoculate roots of At grown in 1g to determine the effect of microgravity upon the inherent virulence or beneficial effects. Based on recent reports of increased virulence of S. typhimurium, P. aeruginosa, and S. Pneumoniae in reduced gravity, differences in microbial pathogenic responses and host plant systemic acquired resistance are expected. The focus of this project within MuRGE involved the development P. indica culture media evaluation and microscopy protocol development. High, clean spore harvest yields for the detection of fungi-plant interactions microscopically was the immediate goal of this experiment.

Haire, Timothy C.↗
NASA NTRS2010

Mutualism in a Reduced Gravity Environment (MuRGE)

MuRGE (Mutualism in a Reduced Gravity Environment) is a NASA flight-research experiment to investigate the microgravity effects associated with cell-cell communication and beneficial microbe-host interactions using a plant-fungal model system. This investigation will use a clinostat, an instrument that slowly rotates the plants to negate the effects of gravitational pull on plant growth (gravitropism) and development, to simulate microgravity. I will be using the endophytic fungus Piriformospora indica (Pi) and the model plant species Arabidopsis thaliana (At). P. indica has been shown to colonize roots of various plant species, including A. thaliana, and to increase plant growth and resistance to stress. The fungus has the ability to grow from spores or in axenic cultures without the presence of a host. P. indica spores and P. indica extract will be used to inoculate Arabidopsis seeds germinated on a clinostat in order to determine if simulated microgravity affects the interaction between the fungus and its plant host.

Patel, Karishma K.↗
NASA NTRS2010

[Mutualism in a Reduced Gravity Environment (MuRGE)]

MuRGE (Mutualism in a Reduced Gravity Environment) is a NASA flight-research experiment to investigate the microgravity effects associated with cell-cell communication and beneficial microbe-host interactions using a plant-fungal model system. This investigation will use a clinostat, an instrument that slowly rotates the plants to negate the effects of gravitational pull on plant growth (gravitropism) and development, to simulate microgravity. I will be using the endophytic fungus Piriformospora indica (Pi) and the model plant species Arabidopsis thaliana (At). P. indica has been shown to colonize roots of various plant species, including A. thaliana, and to increase plant growth and resistance to stress. The fungus has the ability to grow from spores or in axenic cultures without the presence of a host. P. indica spores and P. indica extract will be used to inoculate Arabidopsis seeds germinated on a clinostat in order to determine if simulated microgravity affects the interaction between the fungus and its plant host.

Patel, Karishma↗
NASA NTRSDate not supplied

The Effects of Plasma Application on Radish Seeds with Implications for Space Crop Production

In extended space missions, the astronaut diet will consist mostly of prepackaged foods. This could result in nutritional deficiencies due to the gradual breakdown of certain vitamins. To address this deficiency, fresh produce must be grown from seed during spaceflight. Stored seeds, however, can be vulnerable to microbial contamination which could jeopardize plant health and crop food safety. To alleviate this concern, the current practice is to sanitize seeds on the ground before spaceflight to the International Space Station (ISS). Methods of seed sanitization include alcohol soaking and chlorine gas fumigation, which have harmful effects on the environment and human health. Plasma application is a new sanitization approach that avoids these negative side effects while potentially elevating germination rate and improving growth rate; yet plasma application requires specific exposure time, power, and pressure to achieve these benefits. In the present study, Raphanus sativus ‘Cherry Belle’ radish seeds were exposed to either low pressure (Diener) plasma or atmospheric pressure plasma for varying increments of time (30s-1200s). Data collected includes immediate germination rate (viability) and microbial log reduction. Additionally, seeds were stored for later germination rate testing. Microbial assay controls have highlighted variability in initial microbial load between individual seeds, and further work is being performed to determine the standard microbial load of an untreated seed. In some samples, a negative log reduction was observed after plasma treatment encouraging further study to determine if the plasma is perforating the seed coat and releasing endophytic microbes. Treatment with Diener plasma shows promising microbial log reduction, but lower viability. In contrast, treatment with atmospheric pressure plasma offers high viability, but poor microbial log reduction. Further study will aim at determining whether plasma treatment is effective at not only sanitization, but sterilization. This research was funded by a NASA grant at the Kennedy Space Center.

Plasma↗
NASA NTRSDate not supplied

The Effects of Plasma Application on Radish Seeds with Implications for Space Crop Production

In extended space missions, the astronaut diet will consist mostly of prepackaged foods. This could result in nutritional deficiencies due to the gradual breakdown of certain vitamins. To address this deficiency, fresh produce must be grown from seed during spaceflight. Stored seeds, however, can be vulnerable to microbial contamination which could jeopardize plant health and crop food safety. To alleviate this concern, the current practice is to sanitize seeds on the ground before spaceflight to the International Space Station (ISS). Methods of seed sanitization include alcohol soaking and chlorine gas fumigation, which have harmful effects on the environment and human health. Plasma application is a new sanitization approach that avoids these negative side effects while potentially elevating germination rate and improving growth rate; yet plasma application requires specific exposure time, power, and pressure to achieve these benefits. In the present study, Raphanus sativus ‘Cherry Belle’ radish seeds were exposed to either low pressure (Diener) plasma or atmospheric pressure plasma for varying increments of time (30s-1200s). Data collected includes immediate germination rate (viability) and microbial log reduction. Additionally, seeds were stored for later germination rate testing. Microbial assay controls have highlighted variability in initial microbial load between individual seeds, and further work is being performed to determine the standard microbial load of an untreated seed. In some samples, a negative log reduction was observed after plasma treatment encouraging further study to determine if the plasma is perforating the seed coat and releasing endophytic microbes. Treatment with Diener plasma shows promising microbial log reduction, but lower viability. In contrast, treatment with atmospheric pressure plasma offers high viability, but poor microbial log reduction. Further study will aim at determining whether plasma treatment is effective at not only sanitization, but sterilization. This research was funded by a NASA grant at the Kennedy Space Center.

Space Crop Production↗
DOE OSTI2024

Needle bacterial community structure across the species range of limber pine

Abstract Bacteria on and inside leaves can influence forest tree health and resilience. The distribution and limits of a tree species’ range can be influenced by various factors, with biological interactions among the most significant. We investigated the processes shaping the bacterial needle community across the species distribution of limber pine, a widespread Western conifer inhabiting a range of extreme habitats. We tested four hypotheses: (i) Needle community structure varies across sites, with site-specific factors more important to microbial assembly than host species selection; (ii) dispersal limitation structures foliar communities across the range of limber pine; (iii) the relative significance of dispersal and selection differs across sites in the tree species range; and (iv) needle age structures bacterial communities. We characterized needle communities from the needle surface and tissue of limber pine and co-occurring conifers across 16 sites in the limber pine distribution. Our findings confirmed that site characteristics shape the assembly of bacterial communities across the host species range and showed that these patterns are not driven by dispersal limitation. Furthermore, the strength of selection by the host varied by site, possibly due to differences in available microbes. Our study, by focusing on trees in their natural setting, reveals real needle bacterial dynamics in forests, which is key to understanding the balance between stochastic and deterministic processes in shaping forest tree-microbe interactions. Such understanding will be necessary to predict or manipulate these interactions to support forest ecosystem productivity or assist plant migration and adaptation in the face of global change.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Complete genomes of Mucilaginibacter sabulilitoris SNA2 and Mucilaginibacter sp. cycad4: microbes with the potential for plant growth promotion

Mucilaginibacter species have been isolated from various environments, often in association with plants. Here, we report the complete genomes of Mucilaginibacter sabulilitoris SNA2 and Mucilaginibacter sp. cycad4. The former is the first available for that species, and based on 16S sequence analysis, the latter strain is likely a new species.

Mucilaginibacter↗
DOE OSTI2024

Fungal elemental profiling unleashed through rapid laser-induced breakdown spectroscopy (LIBS)

ABSTRACT Elemental profiling of fungal species as a phenotyping tool is an understudied topic and is typically performed to examine plant tissue or non-biological materials. Traditional analytical techniques such as inductively coupled plasma–optical emission spectroscopy (ICP-OES) and inductively coupled plasma–mass spectrometry (ICP-MS) have been used to identify elemental profiles of fungi; however, these techniques can be cumbersome due to the difficulty of preparing samples. Additionally, the instruments used for these techniques can be expensive to procure and operate. Laser-induced breakdown spectroscopy (LIBS) is an alternative elemental analytical technique—one that is sensitive across the periodic table, easy to use on various sample types, and is cost-effective in both procurement and operation. LIBS has not been used on axenic filamentous fungal isolates grown in substrate media. In this work, as a proof of concept, we used LIBS on two genetically distinct fungal species grown on a nutrient-rich and nutrient-poor substrate media to determine whether robust elemental profiles can be detected and whether differences between the fungal isolates can be identified. Our results demonstrate a distinct correlation between fungal species and their elemental profile, regardless of the substrate media, as the same strains shared a similar uptake of carbon, zinc, phosphorus, manganese, and magnesium, which could play a vital role in their survival and propagation. Independently, each fungal species exhibited a unique elemental profile. This work demonstrates a unique and valuable approach to rapidly phenotype fungi through optical spectroscopy, and this approach can be critical in understanding these fungi's behavior and interactions with the environment. IMPORTANCE Historically, ionomics, the elemental profiling of an organism or materials, has been used to understand the elemental composition in waste materials to identify and recycle heavy metals or rare earth elements, identify the soil composition in space exploration on the moon or Mars, or understand human disorders or disease. To our knowledge, ionomic profiling of microbes, particularly fungi, has not been investigated to answer applied and fundamental biological questions. The reason is that current ionomic analytical techniques can be laborious in sample preparation, fail to measure all potential elements accurately, are cost-prohibitive, or provide inconsistent results across replications. In our previous efforts, we explored whether laser-induced breakdown spectroscopy (LIBS) could be used in determining the elemental profiles of poplar tissue, which was successful. In this proof-of-concept endeavor, we undertook a transdisciplinary effort between applied and fundamental mycology and elemental analytical techniques to address the biological question of how LIBS can used for fungi grown axenically in a nutrient-rich and nutrient-poor environment.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Fungal elemental profiling unleashed through rapid laser-induced breakdown spectroscopy (LIBS)

Elemental profiling of fungal species as a phenotyping tool is an understudied topic and is typically performed to examine plant tissue or non-biological materials. Traditional analytical techniques such as inductively coupled plasma–optical emission spectroscopy (ICP-OES) and inductively coupled plasma–mass spectrometry (ICP-MS) have been used to identify elemental profiles of fungi; however, these techniques can be cumbersome due to the difficulty of preparing samples. Additionally, the instruments used for these techniques can be expensive to procure and operate. Laser-induced breakdown spectroscopy (LIBS) is an alternative elemental analytical technique—one that is sensitive across the periodic table, easy to use on various sample types, and is cost-effective in both procurement and operation. LIBS has not been used on axenic filamentous fungal isolates grown in substrate media. In this work, as a proof of concept, we used LIBS on two genetically distinct fungal species grown on a nutrient-rich and nutrient-poor substrate media to determine whether robust elemental profiles can be detected and whether differences between the fungal isolates can be identified. This data set contains the raw LIBS spectral data for the summarized results described inRush, et. al. 2024

elemental profiling↗