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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 433 records · Page 24
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Accelerating the identification of novel secondary metabolites in bioenergy plant root exudates using MicroED

Small molecule metabolites drive inter- and intraspecies communication and dependencies in diverse biological systems, yet a large proportion of these important chemical compounds remain uncharacterized in plants and microbes. Approximately 90% of the metabolites in root exudate profiles are unknown compounds, despite the importance of root exudate composition in plant-microbe interactions. We need advanced analytical capabilities that will support rapid discovery and structural elucidation of metabolites from biological samples that may be limited in quantity and high in complexity. To fill this gap, this project aimed to develop an integrated workflow involving metabolite extraction, separation, and crystallization from plant root exudates followed by characterization using nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and microcrystal electron diffraction (MicroED). Using crude root exudates from sorghum, this project successfully developed higher throughput exudate fractionation strategies to obtain pure compounds for crystallization and identified crystals in multiple fractions that diffracted. Additional efforts to increase the throughput of high-quality crystal generation for MicroED, such as crystallization screening and crystallization chaperone exploration, will be needed to further advance root exudate metabolite identification. The overall optimized sample preparation process can then be integrated with the existing data collection and data analysis pipelines for MicroED at PNNL to facilitate more rapid natural product discovery.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

APPLICATION OF INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY IN THE ANALYSIS OF PLUTONIUM CONTENT OF FAST CRITICAL ASSEMBLY FUEL DISPOSITION

Outline • Fast Critical Assembly (FCA) Plutonium Fuel Disposition • Brief campaign overview • Savannah River National Laboratory • Analytical capabilities and instrumentation • Typical FCA sample analysis process • Plutonium analysis by quadrupole-inductively coupled plasma-mass spectrometry (Q-ICP-MS) • Method description/development • Instrumentation • Data/control charts

Bonilla, Henry J. [Savannah River National Laborat↗
DOE OSTI2025

High-Throughput Characterization Tools/Algorithms To Outline Porosity Variability in AM Samples as a Function of Processing Conditions

This report documents the development and deployment of advanced algorithms and tools that enable high-throughput characterization for metal additive manufacturing (AM), with a particular focus on process parameter optimization and material/part qualification for nuclear applications. While the method ologies presented support diverse characterization techniques, the majority of the work is centered on AI-driven algorithms for X-ray computed tomography (XCT) to accelerate defect detection and materials analysis at scale.

36 MATERIALS SCIENCE↗
DOE OSTI2026

Zeteotech, LLC TRGR Project Final Report

Reliable detection of aerosolized pathogens is difficult due to need to distinguish between the benign bioaerosols such as dander and pollen and the thousands of pathogens capable of infecting people. Accurate identification of airborne pathogens of concern in the past has required the collection of aerosol samples in a filter, periodic collection of the samples, and processing and identification in the laboratory. This process is labor intensive and expensive. Additionally, this method necessarily has a time to detection window of hours to days depending on the collection frequency. Biological pathogens have an incubation period before the onset of symptoms and severe health effects and/or mortality, people will typically be exposed to them without realizing it. This has resulted in a detect to treat strategy for protection against bio releases. While prophylactic measures can still be effective over these time scales, reducing the time to detection will significantly improve the effectiveness of these measures and subsequently reduce the consequences of a release. Various attempts to reduce the time to detection and identification have been plagued by highly undesirable false-positives which degrade confidence in the system. Zeteotech, LLC has developed a mass spectrometer based bioaerosol sensing system which is capable of autonomously identifying airborne pathogens of concern and alert authorities within minutes instead of hours to days. They have deployed these instruments to protect high-risk facilities by alerting authorities of public health events and intentional bioterrorism events in near real time. This makes it possible to more accurately identify the time and location of the release and minimize the number of people that are exposed through prompt quarantining of affected areas.

47 OTHER INSTRUMENTATION↗
DOE OSTI2026

Microbial Co-Culture Control Proteomics (MC-DP1)

Co-cultured S. elongatus PCC 7942 CscB/SPS and R. toruloides IFO0880 presented as an effective photosynthesis-driven biofuel production platform. The goal of this experiment was to understand the molecular mechanism of this co-culture system at redox post-translational modification level. The redox proteome of the co-cultured strains was compared to a mono-cultured S. elongatus in light or dark conditions. Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify modified cysteines at proteome level. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.

Source record↗
DOE OSTI2025

RhizoGrid Indexed Sorghum Rhizosphere Multi-Omics

PerCon SFA project data dentification of spatially resolved biomarkers of drought in Sorghum bicolor rhizosphere molecular-microbe interactions using a novel root cartography "RhizoGrid" system for sampling plants under drought and control conditions across 10 equally sized root zone environments (4 quadrants each). Each quadrant was sampled and processed for 16S amplicon, metabolomics, and X-ray computed tomography (XCT). Data download includes experimental metadata and results files for 16S rRNA sequence analysis of microbial community assembly (processed data files), liquid chromatography mass spectrometry (LC-MS) metabolomics analysis of microbial community root exudates (processed data files), X-ray computed tomography (XCT) spatial gradient analysis (raw and processed data files) of microbial community composition, and related computational modeling outputs.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2)

The purpose of this experiment was to examine the redox proteome of S. elongatus PCC 7942 CscB/SPS under different light conditions (light and dark) and culture conditions (dense and dilute optical density). Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify protein cysteines which were differentially oxidized under four conditions. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1973

Photographic consulting services for earth resources program at NASA Goddard Space Flight Center

A comparison of prints from ERTS photography revealed significant differences in the image quality produced at GSFC, the EROS facility at Sioux Falls, and Eastman Kodak in Rochester. Based on this survey photographic quality was improved significantly at Sioux Falls, and improvements were suggested for optical and contact printers at the GSFC. Based on a test in which 365 meters of Eastman Electron Recording Film (ESTAR Base) SO-438 were processed the 1-sigma variation in process control from all sources is about + or - 0.04 at a density of 1.0. Variations from film and from processing were approximately equal contributors to the overall tolerance. Distortion produced by the Kodak Colorado printer at GSFC covers a range of about 0.04%, a small value compared to that caused by other factors in the ERTS system. The edge fringing inherent in the color film materials used at GSFC also seems to be a small factor in the unsharpness of ERTS color prints. MTF and granularity measurements were made on processed film samples supplied by GSFC. MTF values were above 0.70 out to 42 cycles/mm for all stages of contact printing in the ERTS system.

Source record↗
NASA NTRS1973

Chemical pump study for Pioneer Venus program

Two chemical pumps were designed for the Pioneer Venus large probe mass spectrometer. Factors involved in the design selection are reviewed. One pump is designed to process a sample of the Venus atmosphere to remove the major component, carbon dioxide, so that the minor, inert components may be measured with greater sensitivity. The other pump is designed to promote flow of atmospheric gas through a pressure reduction inlet system. This pump, located downstream from the mass spectrometer sampling point, provides the pressure differential required for flow through the inlet system. Both pumps utilize the reaction of carbon dioxide with lithium hydroxide. The available data for this reaction was reviewed with respect to the proposed applications, and certain deficiencies in reaction rate data at higher carbon dioxide pressures noted. The chemical pump designed for the inert gas experiment has an estimated volume of 30 cu cm and weight of 80 grams, exclusive of the four valves required for the operation. The chemical pump for the pressure reduction inlet system is designed for a total sample of 0.3 bar liter during the Venus descent.

Rotheram, M.↗
NASA NTRS1973

The data processor from the small scientific satellite

A reprogrammable data system aboard a small scientific satellite is described that samples and processes magnetospheric measurements for transmission to the ground. The lightweight configuration of the data system is made up of the program memory, data storage, input/output module, and a central processing unit. The system is designed for multiple missions.

Mccain, H. G.↗
NASA NTRS1973

The distribution of minor constituents in the stratosphere and lower mesosphere

The complex circulation processes within the stratosphere and mesosphere have been clarified by recent studies. The distribution of minor constituents in the middle atmosphere is significantly influenced by these transport processes. Rocket sampling results are discussed, giving attention to the sampling method, noble gases, methane, water vapor, molecular hydrogen, and carbon dioxide.

Martell, E. A.↗
NASA NTRS1975

Melting behavior and phase relations of lunar samples

Cooling rate studies of 12002 were conducted and the results interpreted in terms of the crystallization history of this rock and certain other picritic Apollo 12 samples. Calculations of liquid densities and viscosities during crystallization, crystal settling velocities, and heat loss by the parent rock body are discussed, as are petrographic studies of other Apollo 12 samples. The process of magmatic differentiation that must have accompanied the early melting and chemical fractionation of the moon's outer layers was investigated. The source of regions of both high- and low-titanium mare basalts were also studied.

Hays, J. F.↗
NASA NTRS1975

Mineralogy of the Ibitira eucrite and comparison with other eucrites and lunar samples

Single crystal X-ray and energy dispersive electron-probe techniques are used to study the mineralogy and track history of Ibitira. The mineralogical features of the Ibitire eucrite are described and examined in relation to data for other eucrites and howardites and lunar samples. The processes which transformed a presumed igneous texture into a more complex one are discussed.

Steele, I. M.↗
NASA NTRS1976

Ion beam texturing

A microscopic surface texture is created by sputter etching a surface while simultaneously sputter depositing a lower sputter yield material onto the surface. A xenon ion beam source has been used to perform this texturing process on samples as large as three centimeters in diameter. Ion beam textured surface structures have been characterized with SEM photomicrographs for a large number of materials including Cu, Al, Si, Ti, Ni, Fe, Stainless steel, Au, and Ag. Surfaces have been textured using a variety of low sputter yield materials - Ta, Mo, Nb, and Ti. The initial stages of the texture creation have been documented, and the technique of ion beam sputter removal of any remaining deposited material has been studied. A number of other texturing parameters have been studied such as the variation of the texture with ion beam power, surface temperature, and the rate of texture growth with sputter etching time.

Hudson, W. R.↗
NASA NTRS1977

Ion beam texturing

A microscopic surface texture was created by sputter-etching a surface while simultaneously sputter-depositing a lower sputter yield material onto the surface. A xenon ion-beam source was used to perform the texturing process on samples as large as 3-cm diameter. Textured surfaces have been characterized with SEM photomicrographs for a large number of materials including Cu, Al, Si, Ti, Ni, Fe, stainless steel, Au, and Ag. A number of texturing parameters are studied including the variation of texture with ion-beam powder, surface temperature, and the rate of texture growth with sputter etching time.

Hudson, W. R.↗
NASA NTRS1978

A significant post-launch calibration experiment for the SEASAT-A SAR

A technique for periodically monitoring the impulse response function of the SEASAT-A synthetic aperture radar is outlined that yields most of the significant contributors to the overall instrument transfer function. The essential measurement involves exciting the total SAR system at L-band with a strong point source scatterer, receiving via the normal S-band analog data link, and finally sampling and processing only a small subset of data in the immediate vicinity of the strong point source.

Beal, R. C.↗