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Field Exploration and Life Detection Sampling Through Planetary Analogue Sampling (FELDSPAR).

Exploration missions to Mars rely on rovers to perform analyses over small sampling areas; however, landing sites for these missions are selected based on large-scale, low-resolution remote data. The use of Earth analogue environments to estimate the multi-scale spatial distributions of key signatures of habitability can help ensure mission science goals are met. A main goal of FELDSPAR is to conduct field operations analogous to Mars sample return in its science, operations, and technology from landing site selection, to in-field sampling location selection, remote or stand-off analysis, in situ analysis, and home laboratory analysis. Lava fields and volcanic regions are relevant analogues to Martian landscapes due to desiccation, low nutrient availability, and temperature extremes. Operationally, many Icelandic lava fields are remote enough to require that field expeditions address several sampling constraints that are experienced in robotic exploration, including in situ and sample return missions. The Fimmvruhls lava field was formed by a basaltic effusive eruption associated with the 2010 Eyjafjallajkull eruption. Mlifellssandur is a recently deglaciated plain to the north of the Myrdalsjkull glacier. Holuhraun was formed by a 2014 fissure eruptions just north of the large Vatnajkull glacier. Dyngjusandur is an alluvial plain apparently kept barren by repeated mechanical weathering. Informed by our 2013 expedition, we collected samples in nested triangular grids every decade from the 10 cm scale to the 1 km scale (as permitted by the size of the site). Satellite imagery is available for older sites, and for Mlifellssandur, Holuhraun, and Dyngjusandur we obtained overhead imagery at 1 m to 200 m elevation. PanCam-style photographs were taken in the field by sampling personnel. In-field reflectance spectroscopy was also obtained with an ASD spectrometer in Dyngjusandur. All sites chosen were 'homogeneous' in apparent color, morphology, moisture, grain size, and reflectance spectra at all scales greater than 10 cm. Field lab assays were conducted to monitor microbial habitation, including ATP quantification, qPCR for fungal, bacterial, and archaeal DNA, and direct cell imaging using fluorescence microscopy. Home laboratory analyses include Raman spectroscopy and community sequencing. ATP appeared to be significantly more sensitive to small changes in sampling location than qPCR or fluorescence microscopy. Bacterial and archaeal DNA content were more consistent at the smaller scales, but similarly variable across more distant sites. Conversely, cell counts and fungal DNA content have significant local variation but appear relatively homogeneous over scales of 1 km. ATP, bacterial DNA, and archaeal DNA content were relatively well correlated at many spatial scales. While we have observed spatial variation at various scales and are beginning to observe how that variation fluctuates over time as biodiversity recovers after an eruption, we do not yet fully understand what parameters lead to the observed spatial variation. Home laboratory analyses will help us further understand the elemental and structural composition of the basaltic matrices, but further field analyses are vital for the understanding how temperature, moisture, incident radiation, and so forth influence the habitability of a microclimate.

Field

Comparison of chain sampling plans with single and double sampling plans

The efficiency of chain sampling is examined through matching of operating characteristics (OC) curves of chain sampling plans (ChSP) with single and double sampling plans. In particular, the operating characteristics of some ChSP-0, 3 and 1, 3 as well as ChSP-0, 4 and 1, 4 are presented, where the number pairs represent the first and the second cumulative acceptance numbers. The fact that the ChSP procedure uses cumulative results from two or more samples and that the parameters can be varied to produce a wide variety of operating characteristics raises the question whether it may be possible for such plans to provide a given protection with less inspection than with single or double sampling plans. The operating ratio values reported illustrate the possibilities of matching single and double sampling plans with ChSP. It is shown that chain sampling plans provide improved efficiency over single and double sampling plans having substantially the same operating characteristics.

Stephens, K. S.

Study of sample drilling techniques for Mars sample return missions

To demonstrate the feasibility of acquiring various surface samples for a Mars sample return mission the following tasks were performed: (1) design of a Mars rover-mounted drill system capable of acquiring crystalline rock cores; prediction of performance, mass, and power requirements for various size systems, and the generation of engineering drawings; (2) performance of simulated permafrost coring tests using a residual Apollo lunar surface drill, (3) design of a rock breaker system which can be used to produce small samples of rock chips from rocks which are too large to return to Earth, but too small to be cored with the Rover-mounted drill; (4)design of sample containers for the selected regolith cores, rock cores, and small particulate or rock samples; and (5) design of sample handling and transfer techniques which will be required through all phase of sample acquisition, processing, and stowage on-board the Earth return vehicle. A preliminary design of a light-weight Rover-mounted sampling scoop was also developed.

Mitchell, D. C.

Overview of the Digitization Workflow Post Image Acquisition of Apollo Lunar and Antarctic Meteorite Samples Using Agisoft Photoscan for the NASA 3D Astromaterials Virtual Samples Collection

The 3D Virtual Astromaterials Samples (3DVAS) collection is a multi-year funded project to create a digital database of sixty Apollo Lunar and Antarctic Meteorite samples following non-destructive documentation conservation protocols. After initial image processing, the photos are evaluated and processed using unique structure-from-motion photogrammetric techniques in a high performance modelling software designed to create a 3D model from 2D images: Agisoft Photoscan Pro. Agisoft Photoscan Pro uses image processing algorithms and techniques originating in computer vision to resolve 3D models for accurate and detailed visualization of a subject. The software provides a stepwise process that is tailored per model based on spatial and specular reflectance properties, for example. The process includes: photo alignment, creation of a dense point cloud, mesh, and finally texture. Photo alignment is dependent on model properties. The 3DVAS process requires a special rotation platform with calibrated photogrammetric targets, specific distance rotation protocols, and a contrasting background for alignment and scale accuracy. As a result of the photographic process, alignment will complete with two mirrored hemispheres that, in a sense, represent the 2D images overlapping to create a 3D model. Each dense point cloud is analyzed with provided statistical measures in a gradual selection process to eliminate outliers. The point cloud is reduced to include only data valuable to the final model. When a precise dense point cloud is achieved, a mesh and texture are applied. Each model is scaled with scale bar accuracies within 100 microns. Each sample has its own intimate process for modelling; there is no standard for the parameters required in the final creation of a high resolution model. By processing multiple samples, a skill is gained in practice to allow a close definition of the original sample and will result in the most detailed version of the sample shell. This process completes one-fifth of the 3DVAS protocol for providing accurate digital documentation. Each model shell is merged with X-ray Computed Tomography data to create a full volumetric sample. All 3DVAS data will be served on NASA's Astromaterials Acquisition and Curation website with an early subset of data available in 2019 and the 3D Virtual Astromaterials Samples Collection launch in 2020.

Thomas, Andi B.

Mars Sample Return: Risk Management & Sample Safety Assessment

Returning samples from Mars has long been a major planetary science objective due to the high scientific value and transformative potential of the resulting data. An exciting dimension of this objective is the potential for the detection of ancient microbiological life, and the possibility of improving our understanding of the evolution of habitable environments on Mars and the development of life on Earth. To ensure that returned samples meet stringent planetary protection requirements and do not expose Earth to potential biohazards, the joint NASA/ESA Sample Receiving Project (SRP) assembled the Sample Safety Assessment Protocol Tiger Team (SSAP-TT). Members were recruited with the specific goal of creating a multi-disciplinary and internationally distributed team of experts in their respective fields across the federal government, academia, and private industry. This team was chartered with reassessing previous sample safety assessment strategies, defining what constitutes a biological hazard, developing a protocol to test for potential biohazards, and establishing a statistical framework to determine if samples are “safe” for release. The team developed a three-step protocol, supported by a Bayesian statistical framework, to assess whether returned samples contain potential biohazards that could present a risk to Earth’s biosphere. Initial conclusions indicated that an effective and comprehensive safety assessment protocol is feasible using modern techniques and does not require an excessive amount of sample consumption or traditional microbiological detection methodology. Herein, we will present an overview of the MSR SRP, the proposed safety assessment protocol, and how aspects of this novel approach can be applied to biological assessment in healthcare product manufacturing practices.

Alvin L Smith

Analysis of the Touch-And-Go Surface Sampling Concept for Comet Sample Return Missions

This paper studies the Touch-and-Go (TAG) concept for enabling a spacecraft to take a sample from the surface of a small primitive body, such as an asteroid or comet. The idea behind the TAG concept is to let the spacecraft descend to the surface, make contact with the surface for several seconds, and then ascend to a safe location. Sampling would be accomplished by an end-effector that is active during the few seconds of surface contact. The TAG event is one of the most critical events in a primitive body sample-return mission. The purpose of this study is to evaluate the dynamic behavior of a representative spacecraft during the TAG event, i.e., immediately prior, during, and after surface contact of the sampler. The study evaluates the sample-collection performance of the proposed sampling end-effector, in this case a brushwheel sampler, while acquiring material from the surface during the contact. A main result of the study is a guidance and control (G&C) validation of the overall TAG concept, in addition to specific contributions to demonstrating the effectiveness of using nonlinear clutch mechanisms in the sampling arm joints, and increasing the length of the sampling arms to improve robustness.

Touch-and-Go (TAG)

Two-stage chain sampling inspection plans with different sample sizes in the two stages

A further generalization of the family of 'two-stage' chain sampling inspection plans is developed - viz, the use of different sample sizes in the two stages. Evaluation of the operating characteristics is accomplished by the Markov chain approach of the earlier work, modified to account for the different sample sizes. Markov chains for a number of plans are illustrated and several algebraic solutions are developed. Since these plans involve a variable amount of sampling, an evaluation of the average sampling number (ASN) is developed. A number of OC curves and ASN curves are presented. Some comparisons with plans having only one sample size are presented and indicate that improved discrimination is achieved by the two-sample-size plans.

Stephens, K. S.

How successful were the lunar sampling tools: Implications for sampling Mars

Like the Mars Sample Return endeavor, the Apollo lunar-sample program began with definition of strategy for sample collection and of scientific requirements for sampling hardware design. Several lessons can be illustrated by specific tools. The evolution of drive tubes from narrow 2 cm diameter, thick-walled tubes (used on Apollo 11, 12 and 14) to 4 cm diameter, thin-walled tubes used on Apollo 15, 16, and 17) as an example of the improvements made possible during multiple missions. The original Apollo 11 drive tube was designed to work in fluffy soil; thus, only 50 percent of the relatively dense lunar soil was recovered, and the core was distorted. The final configuration resulted in nearly 100 pct recovery with little distortion. The surface samplers (Contact Soil Sampling Devices) were designed to collect the upper 100 micrometer or the upper 1 mm of soil. It was over 2 years after the mission before these particularly specific samplers were opened because interest in them waned. Both core tubes and surface samplers were difficult to open in the laboratory. The Apollo Lunar Sample Return Containers (ALSRCs) were constructed with one indium and 2 Viton seals. They were closed on the lunar surface. Interior container pressures measured upon return to the laboratory indicate that these seals were not reliable in the lunar environment. Also, choice of indium as a sealing material interfered with siderophile analyses of samples.

Allton, J. H.

A Trade Study and Metric for Penetration and Sampling Devices for Possible Use on the NASA 2003 and 2005 Mars Sample Return Missions

The scientific exploration of Mars will require the collection and return of subterranean samples to Earth for examination. This necessitates the use of some type of device or devices that possesses the ability to effectively penetrate the Martian surface, collect suitable samples and return them to the surface in a manner consistent with imposed scientific constraints. The first opportunity for such a device will occur on the 2003 and 2005 Mars Sample Return missions, being performed by NASA. This paper reviews the work completed on the compilation of a database containing viable penetrating and sampling devices, the performance of a system level trade study comparing selected devices to a set of prescribed parameters and the employment of a metric for the evaluation and ranking of the traded penetration and sampling devices, with respect to possible usage on the 03 and 05 sample return missions. The trade study performed is based on a select set of scientific, engineering, programmatic and socio-political criterion. The use of a metric for the various penetration and sampling devices will act to expedite current and future device selection.

McConnell, Joshua B.

Sample Manipulation System for Sample Analysis at Mars

The Sample Analysis at Mars (SAM) instrument will analyze Martian samples collected by the Mars Science Laboratory Rover with a suite of spectrometers. This paper discusses the driving requirements, design, and lessons learned in the development of the Sample Manipulation System (SMS) within SAM. The SMS stores and manipulates 74 sample cups to be used for solid sample pyrolysis experiments. Focus is given to the unique mechanism architecture developed to deliver a high packing density of sample cups in a reliable, fault tolerant manner while minimizing system mass and control complexity. Lessons learned are presented on contamination control, launch restraint mechanisms for fragile sample cups, and mechanism test data.

Mumm, Erik

The Evolution of the Orbiting Sample Container for a Future Mars Sample Return

Although NASA has no specific plans at this time to return samples from Mars, the Program Formulation Office of the Mars Exploration Program sponsors ongoing mission concept studies, systems analyses, and technology investments which explore different strategies for the potential return of samples from Mars, consistent with the charter of the program and stated priorities of the science community. A critical component of such a campaign would be an Orbiting Sample container (OS), which would contain the Mars samples to be returned to Earth. This paper discusses the most recent efforts by the JPL’s Mars Formulation Office to mature an OS design planned for use on a potential Mars Sample Return (MSR) mission. Similar to the “Decadal Study Architecture” [1], the current MSR architecture envisions as a three-mission campaign with each mission serving a critical role towards returning Martian rock and atmospheric samples back to Earth. An OS would be a central piece of hardware in the proposed MSR architecture due to its interfaces to all the three missions of the potential campaign. Additionally, numerous stakeholders and subsystems such as science and planetary protection impose challenging requirements on the OS’s functions and capabilities. As a result, designing an OS that meets all the requirements is challenging and quite complex. The story of the OS’s evolution from black box concept thru to the current-and-still-maturing baseline design is the focus of this paper. From the OS’s launch off Earth aboard a Sample Retrieval Lander (SRL) through to return to Earth, the design and functional requirements generated by and for each stage of the OS’s mission are discussed. Then, with an understanding of what the OS would be required to do, a mapping of the main requirements to the design features of the current OS concept is explained. Many tests and analyses have been conducted to support and validate the current OS design. Results from test and analysis in the areas of aerothermal, impact dynamics, optical tracking, and radio electromagnetics are presented.

Spencer, David

Sample Return Container Opening Process Concept for Potential Mars Sample Return

Proposed Mars Sample Return (MSR) missions would require a pristine and secure opening method to ensure sample Contamination Control and backward Planetary Protection. In order to reach the samples, a series of five containers would need to be opened inside of a Mars Returned Sample Handling (MRSH) facility. The process would include opening the Containment Assurance Module (CAM) aboard the Earth Entry Vehicle (EEV), the Secondary Containment Vessel (SCV), Primary Containment Vessel (PCV), and the Orbiting Sample (OS) container, and finally removing and opening the Returned Sample Tube Assemblies (RSTA). Some of these containers can be opened by reversing the mechanical assembly process while others would require destructive means. Several containers are still in a conceptual design phase while others are en route to Mars aboard the M2020 rover. In order to inform the design of the containers that are still in the conceptual stages, a study was performed at each level of the containment assembly. The studies were used to determine the least destructive and most robust methods to disassemble and open the containers while minimizing risk to the samples or the personnel. In addition to the envisioned disassembly and breaching method, contingency plans were made for completing the disassembly if damage to the vehicle and containment system occurred.

Liu, Yang

The Dual-Rasp Sampling System Design with Closed Pneumatic Sample Transfer

The novel Dual-Rasp sampling system has been developed for landed missions to low gravity planetary bodies and is particularly well suited for the unique environment of Saturn’s moon Enceladus. The Dual-Rasp sampling system has two counter-rotating rasp cutters that remove material from the surface and direct it into a guide. The cuttings follow the guide into a sample collection cup. When the sampling collection completes, the tool reconfigures by rotating the guide to create a closed circuit for pneumatic sample transfer from the collection cup to science instruments on the lander. A valve opens a gas tank and the gas flows from that tank into the sample collection cup and down rigid tubing to the science instruments chamber on the lander. A two degree of freedom arm with base actuators is used to deploy the sampler and control its sampling location.

Heness, Andrew B.

Development and Demonstration of a Prototype Molten Salt Sampling System

Molten salt reactors (MSRs) offer potential operability and safety advantages when compared to commercial light water reactors (LWRs). However, operating experience with MSRs is sparse in comparison to what exists for LWRs. Further, the chemical and isotopic composition of the fuel and/or coolant salt is dynamic and difficult to characterize continuously, posing potential safety, operability, and safeguards unknowns that need to be addressed. A molten salt sampling system (MSSS) is regarded as a necessary subsystem within first generation MSRs used to obtain samples of salt for chemical and isotopic analysis in support of the need to monitor and control salt composition during operation. The MSSS is being developed using the Safety-in-Design (SiD) methodology, which incorporates incremental integration of safety analysis into the design process. The MSSS conceptual design emerging from the application of the early stages of the SiD methodology consists of a sample collection system and its housing, a freeze port, and inert gas control and delivery systems. This article describes the prototypes developed to test the functions of these MSSS subsystems, presents the results of testing in both dry and molten salt environments (including reliability data collection performed in accordance with the principles of SiD and the development of a semiquantitative fault tree model), and summarizes the opportunities for future design and testing enhancements based on the results of prototype testing.

molten salt reactor

Mars Sample Recovery Helicopter: Rotorcraft to Retrieve the First Samples from the Martian Surface

The Mars Sample Return Mission (MSR) will carry the next set of Mars helicopters, Sample Recovery Helicopters (SRHs), to the Martian surface. After successfully demonstrating extraterrestrial flight in 2021, Ingenuity has acted as a “scout” for the Perseverance rover while the rover gathers samples of Martian soil. In 2028, the MSR mission will launch a lander and two Ingenuity-sized SRHs to retrieve these samples. These will be the first samples of the Martian surface delivered to Earth. The SRH project will maintain heritage from Ingenuity’s design when possible. However, several key changes must be made, including a ground mobility system, a robotic arm for tube manipulation, and the ability to carry the weight of a science payload (the sample tubes). In addition, the onboard software and cameras will be upgraded, and the rotor radius will be increased. Furthermore, new rotor performance and flight dynamics models and thorough characterization of vehicle limits will be required. The new vehicle design will be described, as well as validation and verification efforts to date.

Rotorcraft

Sample Tube Sealing for Future Proposed Mars Sample Return Missions

A key premise of a proposed Sample Caching Rover, a crucial element of the proposed Mars Sample Return (MSR) campaign, is that the samples could be packaged and left on Mars for an extended period of time (at least five Mars years) without loss of scientific value (Fig. 1). The MEPAG E2E-iSAG (2011) concluded that the single most important factor in preserving the scientific integrity of the samples during the interval between their collection and their analysis is effective sealing of the samples.

sample integrity

Introduction to Mars Sample Handling Workshop Series: Overview of Mars Sample Hazard Analysis

Samples returned from Mars should be contained and treated as though potentially hazardous until proven otherwise If sample containment cannot be verified en route to Earth, the sample and spacecraft should either be sterilized in space or not returned to Earth. Integrity of sample containment should be maintained through reentry and transfer to a receiving facility. Controlled distribution of unsterilized materials should only occur if analyses determine the sample not to contain a biological hazard. Planetary protection measures adopted for the first sample return should not be relaxed for subsequent missions without thorough scientific review and concurrence by an appropriate independent body

Rummel, John D.

Thermal design of the sample handling assembly in the sampling and caching subsystem on the Mars 2020 rover

The Mars 2020 Rover is scheduled to land on Mars on February 18, 2021. One of the primary mission objectives for the Mars 2020 Rover is to collect a set of Martian regolith samples for potential future return to Earth. Regolith and rock samples will be collected and placed into sample tubes using a coring drill, located at the end of a large Robotic Arm, on the outside of the rover. Filled sample tubes will be transferred from the outside of the Rover into the Adaptive Caching Assembly (ACA), located inside the Rover chassis, via the Bit Carousel. Once the filled sample tube is brought into the ACA, the Sample Handling Assembly (SHA) will transfer it to all of the internal processing stations of the ACA for volume assessment, sealing and finally drop-off on the Martian surface. The ACA is a volume within the rover chassis that is not temperature-controlled. Actuators inside the SHA and its End Effector (EE) must be warmed to above -55°C and -25°C, respectively, prior to use. This paper discusses the thermal design of the SHA that uses heaters and temperature sensors to warm up and maintain the temperature-sensitive arm components within temperature limits during operation. Thermal performance predictions for Mars surface operations are also presented.

Lee, Chern-Jiin