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NASA NTRSDate not supplied

Preliminary Medical Risk Estimates and Clinical Capability Needs for Artemis IV

BACKGROUND Human exploration spaceflight missions to the Moon and Mars present unprecedented challenges for in- mission medical care. Compared with the ISS, the greater distance from Earth will mean increased mission durations, communication delays, limited to no resupply opportunities, and significant limitations on the evacuation of ill or injured crew. Spacecraft mass, volume, and power will be curtailed while higher demands will be placed on the crew’s knowledge, skills, and abilities. In this higher risk environment, it is important to: a) quantitatively estimate human system risk attributable to medical conditions, a process known as Probabilistic Risk Analysis, and b) use these estimates to inform medical system design. IMPACT (Informing Mission Planning via Analysis of Complex Tradespaces) is a PRA and medical trade space analysis tool developed by NASA to advance exploration mission medical system design. IMPACT v1.0 improves upon and will soon replace NASA’s existing tool, the Integrated Medical Model, with: a novel evidence base baselined to exploration environments; an expanded list of 119 medical conditions; a significant increase in the number of medical resources that can be utilized and in the flexibility of their use; and the modelling of time lost performing mission-specific tasks due to medical conditions. METHODOLOGY: This abstract will present IMPACT estimates of medical system risk and clinical capability needs for the Artemis IV mission. Artemis IV is currently scheduled for 2026 and will visit the Gateway space station in lunar orbit prior to the second lunar landing of the Artemis program. The baseline Artemis IV mission that was modeled was 28 days in duration with phases including Orion outbound, 4 days on the Gateway space station in lunar orbit, 2 crew on the surface of the Moon for approximately one week, an additional 5 days on Gateway, and then return to Earth. This baseline was compared to two alternative 34-day design reference missions (DRMs) that shifted the lunar sortie earlier or later in the mission profile. Assumptions included 2 female and 2 male crew and a notional medical system mass of 25 kg. Medical system risk estimates include loss of crew life (LOCL), consideration of medical evacuation (known as return to definitive care – RTDC), and an estimate of crew time lost due to medical conditions (Task Time Lost – TTL). The presentation will also describe the medical conditions that are the greatest drivers of risk as well as the clinical capabilities and resources that have the largest effect on risk. RESULTS: All three DRMs had very low probability of LOCL from medical conditions, primarily due to short duration. RTDC was also similar across the DRMs. In contrast, TTL was higher in the early lunar sortie DRM due to earlier occurrence of EVA-related medical conditions. Taken as a whole, there was no clinically significant difference in medical risk across the three missions. Results for clinical capabilities and an example medical equipment list will be discussed but were similar across DRMs.

D Hilmers↗
NASA NTRSDate not supplied

NASA's Biosentinel Mission: Lessons Learned and What's Next

In the last two years, two BioSentinel payloads were launched to space. The ISS mission launched in December 2021, and returned to the ground in August 2022 after successfully completing eight biological experiments while validating the different instruments. On the other hand, the deep space mission launched onboard Artemis I in November 2022, and is currently in a heliocentric orbit over 20 million kilometers away from the Earth. Even though all hardware subsystems were validated in deep space, the microfluidic subsystems experienced anomalies throughout the initial 6-month mission. The main goals of this presentation are (1) to present flight data from the deep space payload, including biology, fluidics, electronics, data processing, and mission operations, and (2) to discuss the lessons learned – what worked and what did not – from this unique complex mission, and how these lessons are aiding in the development of the Lunar Exploration Instrument for space biology Applications (LEIA) mission, launching to the lunar surface on a commercial lander in 2026. As of the writing of this abstract, the satellite continues to work nominally, communicating to Earth via the Deep Space Network (DSN) twice per week. Importantly, the mission received and extension to continue recording data on the deep space radiation environment on its way to solar maximum (i.e., higher probability of solar particle events). BioSentinel is supported by NASA Exploration Systems Development Mission Directorate (ESDMD).

BioSentinel↗
NASA NTRSDate not supplied

The Trash Compaction Processing System (TCPS) Technology Demonstrations and Risk Reduction Activities 2023-2024

The Trash Compactor Processing System (TCPS) employs heat and pressure to safely compress spacecraft trash. The Next STEP Phase B Appendix 2 contract was awarded to Sierra Space for the development of ground and flight demonstration hardware, slated for testing on the International Space Station in 2026. Meanwhile, Ames Research Center is actively engaged in risk reduction activities related to in-house hardware, fine-tuning science objectives and test procedures.This paper will discuss the updated TCPS requirements and present the results of the risk reduction activities, which include moisture analysis, component offgas, and aerosol analysis.

Justine Tra-my Richardson↗
NASA NTRSDate not supplied

Improved Spectral and Angular Characterization of Libya-4 and Dome-C for consistent MODIS and VIIRS Radiometric Scaling

The NASA CERES observed SW and LW fluxes are utilized by the climate community for monitoring the Earth’s energy imbalance and for climate model validation. To facilitate seamless flux and cloud properties across MODIS, VIIRS, and geostationary (GEO) imagers the CERES project intercalibrates the imagers directly comparing the coincident ray-matched analogous channel radiances. For CERES edition 4 products, the MODIS, VIIRS and GEO imagers were radiometrically scaled to the Aqua-MODIS C5 calibration reference. There are no direct comparison opportunities between NPP and NOAA20 VIIRS, since they are positioned a half an orbit apart. The previous usage of Aqua-MODIS as a transfer radiometer is coming to an end, since the Aqua orbit is slowly drifting towards the terminator and will be deorbited in 2026. The CERES project will utilize the Libya-4 and Dome-C invariant targets to radiometrically scale between MODIS and VIIRS reflective solar bands. The Libya-4 and Dome-C Earth invariant targets will be characterized by the repeat cycle angular configuration. The target spectral band adjustment factors (SBAF) will be derived using EMIT and DESIS hyper-spectral observations onboard the ISS and will be compared with the existing SCIAMACHY, GOME-2, and Hyperion SBAFs. The DESIS and EMIT SBAF approach can incorporate the future CLARREO CPF SI traceable hyper-spectral measurements allowing the targets to be referenced to an absolute calibration reference. Utilizing the BRDF and atmospheric corrected characterization of one sensor with another will provide the scaling factors. Consistent scaling factors between the two invariant target validates the method.

David R. Doelling↗
NASA NTRS2024

Preliminary Medical Risk Estimates and Clinical Capability Needs for Late Artemis Missions

BACKGROUND Human exploration spaceflight missions to the Moon and Mars present unprecedented challenges for in- mission medical care. Compared with the ISS, the greater distance from Earth will mean increased mission durations, communication delays, limited to no resupply opportunities, and significant limitations on the evacuation of ill or injured crew. Spacecraft mass, volume, and power will be curtailed while higher demands will be placed on the crew’s knowledge, skills, and abilities. In this higher risk environment, it is important to: a) quantitatively estimate human system risk attributable to medical conditions, a process known as Probabilistic Risk Analysis, and b) use these estimates to inform medical system design. IMPACT (Informing Mission Planning via Analysis of Complex Tradespaces) is a PRA and medical trade space analysis tool developed by NASA to advance exploration mission medical system design. IMPACT v1.0 improves upon and will soon replace NASA’s existing tool, the Integrated Medical Model, with: a novel evidence base baselined to exploration environments; an expanded list of 119 medical conditions; a significant increase in the number of medical resources that can be utilized and in the flexibility of their use; and the modelling of time lost performing mission-specific tasks due to medical conditions. METHODOLOGY: This abstract will present IMPACT estimates of medical system risk and clinical capability needs for the Artemis IV mission. Artemis IV is currently scheduled for 2026 and will visit the Gateway space station in lunar orbit prior to the second lunar landing of the Artemis program. The baseline Artemis IV mission that was modeled was 28 days in duration with phases including Orion outbound, 4 days on the Gateway space station in lunar orbit, 2 crew on the surface of the Moon for approximately one week, an additional 5 days on Gateway, and then return to Earth. This baseline was compared to two alternative 34-day design reference missions (DRMs) that shifted the lunar sortie earlier or later in the mission profile. Assumptions included 2 female and 2 male crew and a notional medical system mass of 25 kg. Medical system risk estimates include loss of crew life (LOCL), consideration of medical evacuation (known as return to definitive care – RTDC), and an estimate of crew time lost due to medical conditions (Task Time Lost – TTL). The presentation will also describe the medical conditions that are the greatest drivers of risk as well as the clinical capabilities and resources that have the largest effect on risk. RESULTS: All three DRMs had very low probability of LOCL from medical conditions, primarily due to short duration. RTDC was also similar across the DRMs. In contrast, TTL was higher in the early lunar sortie DRM due to earlier occurrence of EVA-related medical conditions. Taken as a whole, there was no clinically significant difference in medical risk across the three missions. Results for clinical capabilities and an example medical equipment list will be discussed but were similar across DRMs.

D. Hilmers↗
NASA NTRSDate not supplied

Modeling Measurement Error in Dose-Response Models of Community Annoyance to Low-Noise Supersonic Flight

The primary research goal of the forthcoming NASA Quesst mission community test campaign is to collect representative community response data in support of the development of supersonic overflight noise certification standards. Beginning in 2026, NASA will fly the novel X-59 demonstrator aircraft over select communities in United States in order to demonstrate the possibility of low-noise supersonic flight over land and to collect objective measurements and subjective data on the perceptual experience of this new noise source. It is believed that a regression of a binary perceptual response (‘highly annoyed’ or ‘not’) on estimated noise levels (doses, measured in decibels) will provide a useful dose-response relationship for regulators. However, as these estimated doses will be subject to measurement error, naïve estimators of regression coefficients are inconsistent and slopes may be subject to attenuation bias. In this presentation, I contrast functional modeling of measurement error via simulation extrapolation (SIMEX) with structural Bayesian measurement error models. These methods are applied to available data collected during two NASA risk reduction studies in California in 2011 and Texas in 2018. I’ll conclude noting that in the presence of nonnegligible measurement errors, probabilities of annoyance may be overpredicted for low noise levels and underpredicted for high noise levels, therefore, methods of correcting for measurement error will be necessary to improve the utility of the dose-response relationship for policy-making purposes.

simulation↗
NASA NTRSDate not supplied

Machine Learning Algorithms for Alignment Verification of the Roman Space Telescope

The Nancy Grace Roman Telescope is a NASA observatory designed to unravel the secrets of dark energy and dark matter, search for and image exoplanets, and explore many topics in infrared optics. Scheduled to launch no earlier than October 2026, this 2.4 meter aperture telescope has a field of view 100 times greater than the Hubble Space Telescope. The mission is currently in its construction phase, where the telescope and its two instruments will soon be aligned together to ensure proper pupil matching. To help verify this alignment, multiple point sources above the entrance pupil of the telescope will illuminate the optical path through the telescope-instrument system, and shadows of various obstructions in the system will be analyzed using machine learning algorithms to determine the pupil matching error. This presentation discusses the test approach and the machine learning algorithms employed, as well as our uncertainty predictions based on a modeled Monte-Carlo analysis of the test.

Telescope↗
NASA NTRSDate not supplied

Integrated Modeling for Payload Test of the Roman Space Telescope

The Nancy Grace Roman Telescope (RST) is a NASA observatory designed to unravel the secrets of dark energy and dark matter, search for and image exoplanets, and explore many topics in infrared optics. Scheduled to launch in no earlier than October 2026, this 2.4 meter aperture telescope has a field of view 100 times greater than the Hubble Space Telescope. The mission is currently in its construction phase, where integrated modeling between thermal, structural, and optical models of the observatory is necessary to demonstrate science quality images over the range of operational parameters. This presentation discusses the most recent integrated modeling analysis cycle for Roman, including model correlation with our instrument level testing. We include a discussion on improved processes of the handling of the various flows of data between the modeling disciplines and discipline specific monte-carlo analysis predictions. We will finish with the predicted uncertainties and expected performance for our upcoming observatory alignment verification test using machine learning algorithms.

telescope↗
NASA NTRSDate not supplied

Next Generation X-ray Optics for Astrophysics: High Resolution, Light Weight, and Low Cost

Ready a technology with the following features, compared to Chandra’s. Much better angular resolution: - On-axis: Comparable(~0.5”HPD) by 2026, 5Xbetter by ~2030 - Off-axis: 10Xbetter at 10-arcmin off-axis Much lighter weight per unit area–at least 10Xlighter Much lower cost per unit area–at least 10X lower in real year dollars

Silicon X-ray mirrors↗
NASA NTRSDate not supplied

Mission Architecture for the Green Propulsion Dual Mode Mission

Current spacecraft propulsion technologies are broadly divided into chemical and electric propulsion modes, each of which has unique advantages. It is common for both systems to have a place in interplanetary spacecraft, but the size, weight, and power required to carry two separate propulsion systems is extremely limiting for small spacecraft such as CubeSats. The upcoming NASA STMD-funded Green Propulsion Dual Mode (GPDM) mission will demonstrate on-orbit a novel dual-mode propulsion system known as the GPDM Propulsion System that uses the AF-M315E/ASCENT green monopropellant to feed both a chemical 100 mN monopropellant thruster and four electrospray thrusters. GPDM will fly a 6U CubeSat in low Earth orbit and perform orbit-raising and lowering maneuvers to characterize the performance of this dual-mode propulsion technology, enabling a new generation of future interplanetary small satellite explorers. The Georgia Institute of Technology Space Systems Design Laboratory (SSDL) is conducting the design, assembly, integration, testing, and mission operations of the GPDM host spacecraft, as well as the integration of the GPDM Propulsion System payload, designed by NASA’s Marshall Spaceflight Center (MSFC). The NASA Marshall Space Flight Center is overseeing the overall GPDM project as well as specific technology development activities of the GPDM Propulsion System, while electrospray thrusters are supplied by the MIT Space Propulsion Lab, with additional components supplied by MMA Design, Blue Canyon Technologies, Quasonix, Xiphos, and Rubicon Space Systems. The GPDM spacecraft will carry the GPDM Propulsion System into orbit and use a high-power S-band radio to enable real-time operations in low Earth orbit (LEO) via the NASA Tracking and Data Relay Satellite System (TDRSS). The mission is working towards a tentative launch readiness date of August 2025, in support of launch and operations commencing in January 2026. This paper describes GPDM’s overall mission concept of operations, spacecraft overview, and subsystem breakdown.

Green Propulsion Dual Mode↗
NASA NTRS2024

The Roman Space Telescope Observatory Build, Test and Verification Status

The Nancy Grace Roman Space Telescope (“Roman”) was prioritized by the 2010 Decadal Survey in Astronomy and Astrophysics and is NASA’s next astrophysics flagship observatory. Launching no earlier than 2026, it will conduct several wide field and time domain surveys, as well as conduct an exoplanet census. Roman’s large field of view, agile survey capabilities, and excellent stability enable these objectives, yet present unique engineering and test challenges. The Roman Observatory comprises a Spacecraft and the Integrated Payload Assembly (IPA), the latter of which includes the Optical Telescope Assembly (OTA), the primary science Wide Field Instrument, a technology demonstration Coronagraph Instrument, and the Instrument Carrier, which meters the OTA to each instrument. The Spacecraft supports the IPA and includes the Bus, Solar Array Sun Shield, Outer Barrel Assembly, and Deployable Aperture Cover. It provides all required power, command handling, attitude control, communications, data storage, and stable thermal control functions as well as shading and straylight protection across the entire field of regard. This paper presents the Observatory as it begins integration and test, as well as describes key test and verification activities.

Infrared Missions↗
NASA NTRSDate not supplied

Developing Rationale for Experimental Designs Employed During Forthcoming NASA Quesst Mission Community Noise Campaigns

Since the publication of R.A. Fisher’s “The Arrangement of Field Experiments” in 1926, the enumerated principles that contributed to the improvement of agricultural field experiments in the early 20th century (randomization, replication, blocking, and appropriate variation of factors) remain hallmarks of all well-designed clinical trials and scientific endeavors in the 21st century. Beginning in 2026, NASA will conduct community noise campaigns with the first-of-kind X-59 experimental aircraft to 1) demonstrate the possibility of quiet supersonic flight over land and 2) to collect live data about annoyance (a categorical response) in relation to estimated noise levels (an experimental factor) produced by the new noise phenomenon, a low-noise “sonic thump”. The resulting predicted relationship between perceptual response and estimated noise level, e.g., mixed logistic regression or related models in the class of generalized linear (mixed) models, is believed to be a useful policy tool that domestic and international regulators can use when deciding whether existing prohibitions of supersonic flight over land can be replaced with a noise-based limit. The X-59 is engineered with the expectation that annoyance in the tested ranges should be a rare outcome, and, consequently, NASA convened an interdisciplinary team to examine and refine the rationale underlying the experimental design for future community campaigns. In this presentation, we review relevant literature on experiments with rare binary outcomes, relate the deliberations of the NASA team to tenets of good experimental design, and highlight several challenges and operational realities of this ambitious campaign.

Design of Experiments; blocking; randomization; co↗
NASA NTRS2024

Merged and Gridded GPM and Atmospheric River Data Product

The Global Precipitation Measurement (GPM) Mission Core Observatory satellite launched in 2014 as a joint mission between National Aeronautics and Space Administration (NASA) and JAXA. Global Precipitation Measurement (GPM) has, since that time, provided continuous, valuable dual-frequency radar and passive microwave radiometer observations. Here, we introduce a gridded data set of collocated GPM Core Observatory observational products merged with a reanalysis-derived Atmospheric river (AR) data set in the North Atlantic and North Pacific sectors. The three data sets that are merged and gridded are: (a) the NASA Goddard Profiling (GPROF) precipitation product, which uses GPM passive microwave radiometer observations to derive surface precipitation rates, (b) a water vapor data product derived from the GPM Core Observatory radiometer, provided by Remote Sensing Systems (RSS), and (c) the Mattingly et al. (2018, https://doi.org/10.1029/2018jd028714) AR data set that is specifically tuned to the high-latitude regions. This novel merged data set spans from May 2014 to December 2022 with plans to update annually through 2026 at minimum. This gridded product combines RSS passive water vapor and precipitation estimates with coincident AR detection. This data product benefits the scientific community by providing (a) user-friendly gridded satellite data compared to standard satellite data sets, while maintaining high temporal resolution, and (b) coincident satellite observations to assess the link between ARs and precipitation.

Marian E Mateling↗
NASA NTRSDate not supplied

The BioSensor Instrument Beyond LEIA: a Versatile Platform for Lunar Biology

Introduction: The BioSensor is a deep-space-compatible automated microfluidic culturing instrument. While originally designed to measure the effects of deep space radiation on yeast growth for the BioSentinel mission, it has the potential to host a diverse range of life science experiments with single- and/or multi-celled organisms and can be adapted to interface with a diverse range of platforms in both crewed and uncrewed settings. It is therefore a leading candidate for hosting life sciences experimentation associated with a lunar surface habitat. BioSensor design: The function of the BioSensor is to monitor the growth and metabolic activity of samples in batch fluidic culture, without the need for crew involvement. The current configuration houses organisms in 16 wells within 16 microfluidic cards, accommodating a total of 256 samples, replicates, and controls. Each well has an optical system including three LEDs and a photodiode detector to measure absorbance at three wavelengths, enabling measurement of optical density, color change in dyes such as alamarBlue, and bioproduction of pigmented compounds. Organisms are loaded into fluidic wells and air-dried for storage during integration, launch, and transit, then activated by the introduction of culture medium from storage bags via manifolds that fill one card at a time. Temperature is controlled by individual card heaters, and timing of all activities (fluidics fills, optical measurements, temperatures) is directed by an experiment script. The self-contained BioSensor payload is roughly 4U in volume; with electrical/mechanical/thermal interface, e.g. for operation on ISS or a lunar lander, as well as a linear energy-transfer (LET) charged-particle radiation spectrometer, the entire system is closer to 6U. BioSentinel and LEIA: Flown on the ISS and in a deep-space free flyer for BioSentinel, the BioSensor has been modified for use in the LEIA mission, including improvements to reduce the sensitivity to lengthy launch delays. LEIA will monitor yeast growth in the radiation and reduced-gravity environment of the lunar surface no earlier than 2026, on a CLPS lander [4]. Changes include accommodating additional culture media and an additional LED color for a new biological assay (bioproduction of carotenoids-- dietary antioxidants), as well as modifications to the housing to allow late-load biology changeout and improved isolation between fluidics and electronics. Future prospects: Future work with the BioSensor, beyond LEIA, will include expanding the range and diversity of organisms and assays accommodated. Preliminary work has demonstrated the growth of Arabidopsis seedlings in BioSensor fluidic cards, including optical measurements of growth rate over time. Minor modifications could allow measurement of phenotypes related to photosynthetic capacity in both plants and cyanobacteria. The experimental capabilities of the BioSensor could be dramatically increased by introducing the capability for fluorescence measurements, and/or the design of novel biological assays using luminescence. The BioSensor can also be adapted for new platforms and experiment settings; in addition to free-flyer, ISS, and CLPS lander, a preliminary design concept has been developed for crewed deployment directly to the lunar surface. The instrument could be accommodated inside a lunar habitat, where its automated operation would make it an excellent candidate for experiments from fundamental investigations into the response of organisms to lunar surface conditions to applied-science purposes such as screening engineered strains of various organisms for bioproduction capability.

J A Lee↗
NASA NTRSDate not supplied

HERA in the Artemis ERA

As NASA shifts its mission objectives to the lunar surface and the return of humans to the moon in the next few years, analogs that simulate lunar transit or surface exploration are in a unique position to support spaceflight research that is aligned with future Artemis missions, especially a sustained lunar presence. NASA’s spaceflight analogs have historically focused on mission scenarios and operations for Mars exploration, including recent campaigns staged at the Human Exploration Research Analog (HERA) analog at Johnson Space Center. However, beginning in Campaign 8, future HERA missions are planned to mimic lunar operations and support research objectives that directly address knowledge gaps for lunar exploration and habitation. The Research Operations and Integration (ROI) HERA team will present a look-back over HERA mission scenarios and operations supporting NASA’s Human Research Program and international partners’ spaceflight research. An overview of critical capabilities in HERA and other similar analogs will also be presented, such as methods of simulating EVAs. Presenters will then discuss the transition to a high-fidelity lunar surface exploration mission scenario envisioned for Campaign 8, slated to begin in 2026. Operations changes that will be discussed will include simulated lunar surface exploration using a two-person simulated pressurized rover in combination with virtual reality, lunar-relevant communication delays, changes to concepts of how crew communicate with Earth-based mission supports, increased access to family & friends’ communication compared to prior campaigns, and a new mission scenario referencing Artemis mission objectives and tasks. ROI analog mission designs are driven by science objectives. The ROI HERA team will describe how the changes for Campaign 8 support ongoing spaceflight research interests and provide opportunities for investigators to design experiments more closely aligned to lunar missions. A questions and answers session will follow with attendees encouraged to ask questions.

B J Caldwell↗
NASA NTRS2024

Current Status of Martian Moons eXploration (MMX) Contamination Control and Curation Activity

Martian Moons eXploration (MMX) is a sample return mission from the Martian moon Phobos. The MMX spacecraft is scheduled to launch in 2026 and return to Earth in 2031. The main science goals of MMX are “to reveal the origin of the Martian moons and make progress in the understanding of planetary system formation and material transport in the solar system, and to observe processes that impact the circumplanetary and surface environments of Mars”. MMX has two sampling systems: coring (C)-sampler and pneumatic (P)-sampler and plans to bring back >10 g of Phobos sample. The retuned sample in the sample capsule will be transferred to the curation facility in ISAS/JAXA for sample curation and subsequent sample analysis. Contamination control of the sample return mission requires special care to prevent terrestrial contamination to the spacecraft, which would ruin the scientific value of the returned sample. Retaining the pristineness of the retuned sample is an important task of the MMX Curation and Sampler Science teams. The basis of the contamination control is (1) to minimize and understand the nature and amount of contaminants, (2) to perform contamination assessment and evaluate the effect of contaminants in the spacecraft on the retuned sample, (3) to employ a contamination knowledge (CK) material coupon in the spacecraft to identify the contaminants in the returned samples. In the MMX contamination control plan, the allowable contamination level for each contaminant is carefully defined. They are mostly set to be 1/1000 of the expected amount of each material in the returned sample and are divided into two main categories: organic and inorganic. The allowable atmospheric leakage rate to the sample container is also defined. The allowable contamination level of the organic materials is based on the composition of carbonaceous chondrites. The target contaminants are amino acids, aliphatic and aromatic hydrocarbons, carboxylic acids, etc. In case of the inorganic materials, the target contaminants are important elements to permit distinguishing the origin of the Martian moon by nucleosynthetic isotope anomalies (Cr, Ti, and Mo) and to reveal the evolution of the Martian moon by chronology (Hf, W, U, Pb, Rb, Sr, Sm, and Nd). The key instrument of contamination control in the sample return mission is the sampler system. The C-sampler has been developed by JAXA and the P-sampler was provided by Honeybee/NASA. In MMX, materials used in the two samplers (C- and P- sampler) were carefully selected to avoid potential contamination from the design stage of the system. The individual parts of the C-sampler FM (Flight Model) were thoroughly cleaned at the curation facility in ISAS/JAXA by the full-course cleaning procedure, which is an ultrasonic cleaning with organic solvents and ultrapure water in several steps. The equivalent level of cleaning was also carried out on the P-Samper FM as well by Honeybee Robotics in the USA. Now, MMX is in the critical phase for contamination control called ATLO: Assembly, Test, and Launch Operations. During the ATLO phase, sampler FM is constantly purged with nitrogen gas and maintained at positive pressure to prevent environmental contamination. The surrounding environments of the sampler FM are also simultaneously monitored using the CK Monitoring Coupon Set, which consists of several witness materials such as a glass petri dish, sapphire glass disk, and carbon adhesive tape (Figure 1). The detailed environmental assessment of each clean room used for the assembly and test of the sampler FM has also been conducted. This assessment includes microbial analysis, which was performed for OSIRIS-REx. Regarding the sample recovery and sample curation, we have started the designing of Sample Container Disassembling Instrument for the sample recovery from the sample container and the MMX curation chamber for sample curation. The curation protocol for the Phobos returned sample has also been discussed by the MMX Sample Analysis Working Team (SAWT). The MMX curation protocol consists of three phases: (1) quick analysis, (2) pre-basic characterization, and (3) basic characterization. (1) is extraction of the sample gas from the sample container and analysis by mass spectrometry, (2) is observation in bulk level, and (3) is observation in grain level and allocation of the sample aliquots. In parallel with the curation protocol, the returned sample undergoes preliminary examination for scientific investigations to achieve science goals. In addition, the CK witness plates made of sapphire glass are on board the sampler system. The CK witness plates will be recovered from the sampler system and analyzed by SAWT for the assessment of in-flight contamination.

Haruna Sugahara↗
NASA NTRSDate not supplied

A Heritage BioSensor for Lunar Biology Experiments

Introduction: Automated biological experiments on small spacecraft missions have gained prominence over the past decade due to their simplicity, accessibility, and small mass, volume, and power needs. Most recently, the BioSensor microfluidic CubeSat payload aboard BioSentinel used an automated microfluidic cell culture system to study the effects of environmental stressors like deep space radiation and microgravity on yeast growth and metabolism. BioSentinel’s successor, the Lunar Explorer Instrument for space biology Applications (LEIA), will study the effects of lunar gravity and radiation using an improved version of the BioSensor microfluidic platform. The BioSensor payload has great adaptability to host a diverse range of biological experiments with single- and multi-celled organisms in both crewed and uncrewed missions, making it a compelling candidate for future space biology studies in a lunar surface environment. BioSensor Instrumentation on BioSentinel: The first spaceflight mission with the BioSensor, BioSentinel’s biology experiments occurred at three locations -- deep space, ISS and ground. The payload contained 18 microfluidic cards, each featuring 16 growth wells (a total of 288 growth wells). Each well was loaded before launch with desiccated yeast. In space, liquid culture medium (nutrients) was automatically introduced to batches of wells at a time to initiate a series of biology experiments. Temperature was maintained by thin film heaters on both sides of each card. Each well was equipped with three LEDs emitting at 570 nm, 630 nm, and 850 nm, paired with photodetectors to measure cell concentration and the alamarBlue (metabolic indicator dye) color transition from blue to pink. Phenotypic parameters like cell viability, metabolic rate, and generation time can be derived from these measurements. The sequence and timing of fluid fills, optical measurements, and thermal control were stored onboard, but could be updated asynchronously via ground communication. LEIA: LEIA is slated for launch no earlier than 2026 on a CLPS lander. BioSentinel’s BioSensor has been modified for use in LEIA. These improvements include: (a) storage for multiple culture medium types, (b) additional LED color (465 nm) for a new biological assay for antioxidant (carotenoid) production, (c) housing modifications for later biology load before launch, (d) improved isolation between electronic and fluidic components, and (e) improved humidity control for prolonged organism viability in case of post-load launch delay. Future Prospects: The consistent and successful demonstration of complex fluidics platforms alongside reliable instrument operations in a space environment is poised to create strong momentum for BioSensor-based biological experiment payloads. Planned future developments with the BioSensor include extending compatibility to a broader range of organisms and assays. Preliminary work has already demonstrated successful growth of Arabidopsis seedlings in fluidic cards. With a few modifications to the optical assembly, the setup could easily measure photosynthetic traits in plants and cyanobacteria. The addition of fluorescence measurements and generation of novel luminescent assays will elevate BioSensor’s functionality further. Beyond the BioSensor’s potential uses on free-flyer missions, ISS and Gateway, and CLPS landers, deploying the BioSensor to the lunar surface or in an artificial habitat on crewed missions could enable pioneering research on both how life responds to lunar conditions and future bioproduction capabilities making the BioSensor an indispensable tool for future space biology research.

Chinmayee Govinda Raj↗
NASA NTRSDate not supplied

Advanced Supersonic Parachute Inflation Research Experiment-2 (ASPIRE2) Parachute Modeling and Flight Mechanics Performance

The Advanced Supersonic Parachute Inflation Research Experiment-2 (ASPIRE2) program is designed to test and qualify the supersonic parachute for the Mars entry, descent, and landing stage of the Mars Sample Return campaign. The ASPIRE2 test was planned for early 2026 and would have involved testing the parachute at deployments of Mach 2.1 and higher load conditions than previous Mars missions. The ASPIRE2 simulation builds upon a previous test framework from the ASPIRE tests in 2017-2018 used to qualify the Mars 2020 parachute, but due to new requirements, the modeling and simulation for ASPIRE2 has needed development and characterization of different sensitivities. This paper looks at the mission design as of mid-2024 and presents the trade space for qualifying the parachute in a dynamic environment.

Evan Roelke↗