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At least 73 records · Page 4

Density and composition of microorganisms during long-term (418 day) growth of potato using biologically reclaimed nutrients from inedible plant biomass

A study evaluating alternative methods for long term operation of biomass production systems was recently completed at the Kennedy Space Center (KSC). The 418-day study evaluated repeated batch versus mixed-aged production of potato grown on either standard 1/2-strength Hoagland's nutrient solution or solutions including nutrients recycled from inedible plant material. The long term effects of closure and recycling on microbial dynamics were evaluated by monitoring the microbial communities associated with various habitats within the plant growth system (i.e., plant roots, nutrient solution, biofilms within the hydroponic systems, atmosphere, and atmospheric condensate). Plate count methods were used to enumerate and characterize microorganisms. Microscopic staining methods were used to estunate total cell densities. The primary finding was that the density and composition of microbial communities associated with controlled environmental plant growth systems are stable during long term operation. Continuous production resulted in slightly greater stability. Nutrient recycling, despite the addition of soluble organic material from the waste processing system, did not significantly increase microbial density in any of the habitats.

NASA Center KSC↗

Plant size, etc., and aerial films

Chlorophyll concentration of chlorotic leaves, expressed as milligrams of chlorophyll per cubic centimeter of leaf volume, was positively correlated to film density readings. Images on the film became darker as chlorophyll concentration increased. Plant size was not related to density readings.

Gausman, H.↗

Wheat production in controlled environments

The present optimization study for maximum yield and quality conditions in the lunar or Martian Controlled Environment Life Support System (CELSS)-based growth of wheat has determined that, for 23-57 g/sq m per day of edible biomass, minimum CELSS size must be of the order of 12-30 sq m/person. About 600 W/sq m of electricity would be consumed by the artificial lighting required; temperature, irradiance, photoperiod, CO2 levels, humidity, and wind velocity are all controlled. A rock wool plant support allows direct seeding, and densities of up to 10,000 plants/sq m. Densities of up to 2000 plants/sq m appear to increase seed yields.

Salisbury, Frank B.↗

SO2 Plumes Observation with LMOL: Theory, Modeling, and Validation

LMOL, the NASA Langley Mobile Ozone Lidar, is located near NASA’s LaRC steam plant when not deployed in campaigns. The plant produces steam through the incineration of local trash, and its exhaust plume occasionally contains SO2 . SO2 is a known, regulated, pollutant that affects O3 observations in the UV, as is the case with LMOL. In this work, we show how we modified LMOL to detect the plant SO2 plumes and compute its density when the O3 background is stable; we observed densities compatible with what is expected from the typical plume exhaust. The selection of laser lines so that an O3 variation would not get confused with a SO2 detection is explained in details, and a model capable of simulating the Lidar signal with (notably) O3 and SO2 absorption for the validation of the retrieval resolution and uncertainty is presented. Finally, the comparison between the modeled and observed performances of the system is shown: the maximum altitude, resolution, and error in the modeled signal and the observed signals are the same within reasonable margins. This work demonstrates that LMOL is fully capable of working with SO2 . The next step being the addition of an additional laser channel, which is simplified by the tunable aspect of the LMOL laser, to address O3 and SO2 simultaneously, allowing the assessment of SO2 when O3 has variations. Note: This presentation is accompanied by an mp4 video of the given talk.

Guillaume Gronoff↗

Vegetation structural complexity and biodiversity in the Great Smoky Mountains

Vegetation structural complexity and biodiversity tend to be positively correlated, but understanding of this relationship is limited in part by structural metrics tending to quantify only horizontal or vertical variation, and that do not reflect internal structure. We developed new metrics for quantifying internal vegetation structural complexity using terrestrial LiDAR scanning and applied them to 12 NEON forest plots across an elevational gradient in Great Smoky Mountains National Park, USA. We asked (1) How do our newly developed structure metrics compare to traditional metrics? (2) How does forest structure vary with elevation in a high-biodiversity, high topographic complexity region? (3) How do forest structural metrics vary in the strength of their relationships with vascular plant biodiversity? Our new measures of canopy density (Depth) and structural complexity (σDepth), and their canopy height-normalized counterparts, were sensitive to structural variations and effectively summarized horizontal and vertical dimensions of structural complexity. Forest structure varied widely across plots spanning the elevational range of GRSM, with taller, more structurally complex forests at lower elevation. Vascular plant biodiversity was negatively correlated with elevation and more strongly positively correlated with vegetation structure variables. The strong correlations we observed between canopy structural complexity and biodiversity suggest that structural complexity metrics could be used to assay plant biodiversity over large areas in concert with airborne and spaceborne platforms.

Jonathan A. Walter↗

Closed Loop Control Compact Exercise Device for Use on MPCV

Long duration space travel to Mars or to an asteroid will expose astronauts to extended periods of reduced gravity. To combat spaceflight physiological deconditioning, astronauts will use resistive and aerobic exercise regimens for the duration of the space flight to minimize the loss of bone density, muscle mass and aerobic capacity that occurs during exposure to a reduced gravity environment. Unlike the International Space Station (ISS), the mass and volume available for an exercise device in the next generation of spacecraft is limited. Therefore, compact exercise device prototypes are being developed for human in the loop evaluations. The NASA Human Research Program (HRP) is managing Advanced Exercise Concepts (AEC) requirements development and candidate technology maturation for all exploration mission profiles from Multi-Purpose Crew Vehicle (MPCV) exploration missions (e.g., EM-2, up to 21 day) to Mars Transit (up to 1000 day) missions. Numerous technologies have been considered and evaluated against HRP-approved functional requirements and include flywheel, pneumatic and closed-loop microprocessor-controlled motor driven power plants. Motor driven technologies offer excellent torque density and load accuracy characteristics as well as the ability to create custom mechanical impedance (the dynamic relationship between force and velocity) and custom load versus position exercise algorithms. Further, closed-loop motor-driven technologies offer the ability to monitor exercise dose parameters and adapt to the needs of the crewmember for real time optimization of exercise prescriptions. A simple proportional-integral-derivative (PID) controller is demonstrated in a prototype motor driven exercise device with comparison to resistive static and dynamic load set points and aerobic work rate targets. The resistive load term in the algorithm includes a constant force component (Fcmg) as well as inertial component (Fima) and a discussion of system tuning is presented in terms of addressing key functional requirements and human interfaces. The device aerobic modality is modelled as a rowing exercise using ground data sets obtained from Concept 2 rowers as well as competitive rowing1. A discussion of software and electronic implementations are presented which demonstrate unique approaches to meeting the constrained mass, volume and power requirements of the MPCV. . In addition to utilizing traditional PID control, controllers utilizing state feedback with gains solved using a Linear Quadratic Regulator will be developed. Controllability and observability will be utilized to investigate the need for state measurement in the design. As the control system directly interacts with human test subjects, robust methods such as H-infinity are also being investigated.1. Kleshnev V. Biomechanics. In: Rowing, Handbook of Sports Medicine and Science. ed. by Secher N., Voliantis S. IOC Medical Commission, Blackwell Pub. pp. 22-34, 2007

microgravity↗

Using Remote Sensing Mapping and Growth Response to Environmental Variability to Aide Aquatic Invasive Plant Management

Management of aquatic weeds in complex watersheds and river systems present many challenges to assessment, planning and implementation of management practices for floating and submerged aquatic invasive plants. The Delta Region Areawide Aquatic Weed Project (DRAAWP), a USDA sponsored area-wide project, is working to enhance planning, decision-making and operational efficiency in the California Sacramento-San Joaquin Delta. Satellite and airborne remote sensing are used map (area coverage and biomass density), direct operations, and assess management impacts on plant communities. Archived satellite records enable review of results following previous climate and management events and aide in developing long-term strategies. Examples of remote sensing aiding effectiveness of aquatic weed management will be discussed as well as areas for potential technological improvement. Modeling at local and watershed scales using the SWAT modeling tool provides insight into land-use effects on water quality (described by Zhang in same Symposium). Controlled environment growth studies have been conducted to quantify the growth response of invasive aquatic plants to water quality and other environmental factors. Environmental variability occurs across a range of time scales from long-term climate and seasonal trends to short-term water flow mediated variations. Response time for invasive species response are examined at time scales of weeks, day, and hours using a combination of study duration and growth assessment techniques to assess water quality, temperature (air and water), nitrogen, phosphorus, and light effects. These provide response parameters for plant growth models in response to the variation and interact with management and economic models associated with aquatic weed management. Plant growth models are to be informed by remote sensing and applied spatially across the Delta to balance location and type of aquatic plant, growth response to altered environments and phenology. Initial utilization of remote sensing tools developed for mapping of aquatic invasive plants improved operational efficiency in management practices. These assessment methods provide a comprehensive and quantitative view of aquatic invasive plants communities in the California Delta.

Remote Sensing↗

Microgreens Food Safety Evaluation

Microgreens have been recently identified as a new type of pick and eat salad crop that can be utilized in space crop production systems. The majority of traditionally grown leafy green crops can be grown as microgreens, in addition to crops such as legumes (e.g. pea shoots), sunflower, buckwheat, most herbs, and corn, presenting hundreds of microgreen crop options. With a wide variety of flavors, exceptional nutritional density, short growth cycles (7-14 days), and unmatched volume optimization potential, microgreens present as an interesting option for sustainable production of nutritious and flavorful crops in space. The food safety aspects of microgreens have been investigated by USDA in recent years, however, an assessment for crew consumption purposes has not yet been conducted to capture the variety of microgreen cultivar types grown in spaceflight relevant environmental conditions and hardware that could be a part of the astronaut diet. Seed sanitation methods will be developed for each microgreen cultivar to sanitize the seed coat and ensure viable germination and plant growth. Food safety analysis will be performed on 24 different fast-growing cultivars of microgreens by performing aerobic plate counts (APC), fungal counts, and select pathogen screening of edible plant tissue before and after treatment with ProSan sanitizing produce wash. Additional avenues of investigation into microgreens food safety will be undertaken to understand impacts of plant height at time of harvest (able to be manipulated by light spectrum), seed density at time of planting, and role of blue light in food safety metrics of microgreens (n=6-12 microgreen cultivars tested, depending on variable). These findings will be included in the Space Crop Production Hazard Analysis Critical Control Point (HACCP) plan. This new work is currently postponed due to Covid-19.

M E Hummerick↗

The application of LANDSAT-1 imagery for monitoring strip mines in the new river watershed in northeast Tennessee, part 2

The author has identified the following significant results. LANDSAT imagery and supplementary aircraft photography of the New River drainage basin were subjected to a multilevel analysis using conventional photointerpretation methods, densitometric techniques, multispectral analysis, and statistical tests to determine the accuracy of LANDSAT-1 imagery for measuring strip mines of common size. The LANDSAT areas were compared with low altitude measurements. The average accuracy over all the mined land sample areas mapped from LANDSAT-1 was 90%. The discrimination of strip mine subcategories is somewhat limited on LANDSAT imagery. A mine site, whether active or inactive, can be inferred by lack of vegetation, by shape, or image texture. Mine ponds are difficult or impossible to detect because of their small size and turbidity. Unless bordered and contrasted with vegetation, haulage roads are impossible to delineate. Preparation plants and refuge areas are not detectable. Density slicing of LANDSAT band 7 proved most useful in the detection of reclamation progress within the mined areas. For most state requirements for year-round monitoring of surface mined land, LANDSAT is of limited value. However, for periodic updating of regional surface maps, LANDSAT may provide sufficient accuracies for some users.

Shahrokhi, F.↗

Seasat synthetic aperture radar /SAR/ response to lowland vegetation types in eastern Maryland and Virginia

Examination of Seasat SAR images of eastern Maryland and Virginia reveals botanical distinctions between vegetated lowland areas and adjacent upland areas. Radar returns from the lowland areas can be either brighter or darker than returns from the upland forests. Scattering models and scatterometer measurements predict an increase of 6 dB in backscatter from vegetation over standing water. This agrees with the 30-digital number (DN) increase observed in the digital Seasat data. The brightest areas in the Chickahominy, Virginia, drainage, containing P. virginica about 0.4 m high, contrast with the brightest areas in the Blackwater, Maryland, marshes, which contain mature loblolly pine in standing water. The darkest vegetated area in the Chickahominy drainage contains a forest of Nyssa aquatica (water tupelo) about 18 m high, while the darkest vegetated area in the Blackwater marshes contains the marsh plant Spartina alterniflora, 0.3 m high. The density, morphology, and relative geometry of the lowland vegetation with respect to standing water can all affect the strength of the return L band signal.

Krohn, M. D.↗

Potential Sources of Polarized Light from a Plant Canopy

Field measurements have demonstrated that sunlight polarized during a first surface reflection by shiny leaves dominates the optical polarization of the light reflected by shiny-leafed plant canopies having approximately spherical leaf angle probability density functions ("Leaf Angle Distributions" - LAD). Yet for other canopies - specifically those without shiny leaves and/or spherical LADs - potential sources of optically polarized light may not always be obvious. Here we identify possible sources of polarized light within those other canopies and speculate on the ecologically important information polarization measurements of those sources might contain.

Polarized Light↗

Near-term lander experiments for growing plants on Mars: requirements for information on chemical and physical properties of Mars regolith

In order to support humans for long-duration missions to Mars, bioregenerative Advanced Life Support (ALS) systems have been proposed that would use higher plants as the primary candidates for photosynthesis. Hydroponic technologies have been suggested as the primary method of plant production in ALS systems, but the use of Mars regolith as a plant growth medium may have several advantages over hydroponic systems. The advantages for using Mars regolith include the likely bioavailability of plant-essential ions, mechanical support for plants, and easy access of the material once on the surface. We propose that plant biology experiments must be included in near-term Mars lander missions in order to begin defining the optimum approach for growing plants on Mars. Second, we discuss a range of soil chemistry and soil physics tests that must be conducted prior to, or in concert with, a plant biology experiment in order to properly interpret the results of plant growth studies in Mars regolith. The recommended chemical tests include measurements on soil pH, electrical conductivity and soluble salts, redox potential, bioavailability of essential plant nutrients, and bioavailability of phytotoxic elements. In addition, a future plant growth experiment should include procedures for determining the buffering and leaching requirements of Mars regolith prior to planting. Soil physical tests useful for plant biology studies in Mars regolith include bulk density, particle size distribution, porosity, water retention, and hydraulic conductivity.

NASA Center JSC↗

Reflectance of vegetation, soil, and water

The author has identified the following significant results. Iron deficient and normal grain sorghum plants were sufficiently different spectrally in ERTS-1 band 5 CCT data to detect chlorotic sorghum areas 2.8 acres (1.1 hectares) or larger in size in computer printouts of the MSS data. The ratio of band 5 to band 7 or band 7 minus band 5 relates to vegetation ground cover conditions and helps to select training samples representative of differing vegetation maturity or vigor classes and to estimate ground cover or green vegetation density in the absence of ground information. The four plant parameters; leaf area index, plant population, plant cover, and plant height explained 87 to 93% of the variability in band 6 digital counts and from 59 to 90% of the variation in bands 4 and 5. A ground area 2244 acres in size was classified on a pixel by pixel basis using simultaneously acquired aircraft support and ERTS-1 data. Overall recognition for vegetables, immature crops and mixed shrubs, and bare soil categories was 64.5% for aircraft and 59.6% for spacecraft data, respectively. Overall recognition results on a per field basis were 61.8% for aircraft and 62.8% for ERTS-1 data.

Wiegand, C. L.↗

Influence of atmospheric oxygen on leaf structure and starch deposition in Arabidopsis thaliana

Plant culture in oxygen concentrations below ambient is known to stimulate vegetative growth, but apart from reports on increased leaf number and weight, little is known about development at subambient oxygen concentrations. Arabidopsis thaliana (L.) Heynh. (cv. Columbia) plants were grown full term in pre-mixed atmospheres with oxygen partial pressures of 2.5, 5.1, 10.1, 16.2, and 21.3 kPa O2, 0.035 kPa CO2 and the balance nitrogen under continuous light. Fully expanded leaves were harvested and processed for light and transmission electron microscopy or for starch quantification. Growth in subambient oxygen concentrations caused changes in leaf anatomy (increased thickness, stomatal density and starch content) that have also been described for plants grown under carbon dioxide enrichment. However, at the lowest oxygen treatment (2.5 kPa), developmental changes occurred that could not be explained by changes in carbon budget caused by suppressed photorespiration, resulting in very thick leaves and a dwarf morphology. This study establishes the leaf parameters that change during growth under low O2, and identifies the lower concentration at which O2 limitation on transport and biosynthetic pathways detrimentally affects leaf development. Grant numbers: NAG5-3756, NAG2-1020, NAG2-1375.

Non-NASA Center↗

Timber Resources Inventory and Monitoring Joint Research Project

Primary objectives were to develop remote sensing analysis techniques for extracting forest related information from LANDSAT Multispectral Scanner (MMS) and Thematic Mapper data and to determine the extent to which International Paper Company information needs can be addressed with remote sensing information. The company actively manages 8.4 million acres of forest land. Traditionally, their forest inventories, updated on a three year cycle, are conducted through field surveys and aerial photography. The results reside in a digital forest data base containing 240 descriptive parameteres for individual forest stands. The information in the data base is used to develop seasonal and long range management strategies. Forest stand condition assessements (species composition, age, and density stratification) and identification of silvicultural activities (site preparation, planting, thinning, and harvest) are addressed.

Hill, C. L.↗

Testing Metal Chlorides For Use In Sodium-Cell Cathodes

Cyclic voltammetric curves of transition-metal wires in molten NaAlCl4 electrolyte used to eliminate suitability of transition metals as cathodes in sodium cells. Cyclic voltammetry used in conjunction with measurement of galvanostatic polarization curves determines whether given metal chloride suitable as cathode material in such cell. Cells useful in such high-energy-density and high-power-density applications as leveling loads on electric-power plants, supplying power to electric ground vehicles, and aerospace applications.

Bugga, Ratnakumar V.↗

Philadelphia Health & Air Quality: Assessing Land Surface Temperature, Vegetation Cover, and Compounding Vulnerability Factors to Identify High Priority Areas for Cooling Initiatives in Philadelphia, Pennsylvania

Heat is the leading cause of weather-related deaths in the US, with heat-related hospitalizations increasing by 2-5% between 2001-2010. In Philadelphia alone, 137 heat-related deaths were recorded between 2010-2018, while a total of 18 daily temperature records have been set since 2010. Temperature is relatively higher in cities compared to rural areas, a phenomenon known as the urban heat island effect. This effect exaggerates daytime maximum temperatures and nighttime heat retention in urban areas, which increases heat exposure in urban environments and especially impacts vulnerable populations. Vulnerability to heat-related illnesses is determined by a combination of risk factors, such as demographics, socioeconomic status, and preexisting health conditions. This project supported the Philadelphia Department of Public Health and Office of Sustainability by identifying priority areas for cooling interventions, such as heat danger educational outreach and urban tree planting. The team developed heat vulnerability scores for each census tract within Philadelphia. Remotely sensed land surface temperature, normalized difference vegetation index, normalized difference built-up index, normalized difference water index, and albedo data were calculated from Aqua Moderate Resolution Imaging Spectroradiometer and Landsat 8 Operational Land Imager/Thermal Infrared Sensor instruments. These variables were weighted against socioeconomic variables and preexisting health conditions using a principal component analysis. A total of 74 census tracts clustered were identified as high-risk areas for heat-related illnesses. 15 of these census tracts also had very low tree density (lower 20th percentile) and should be targeted for tree planting initiatives. The findings of this project will help target interventions to mitigate heat-related health issues and improve the overall wellness of Philadelphia residents.

Health & Air Quality↗