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All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Black-box optimization of CT acquisition and reconstruction parameters: a reinforcement learning approach

Protocol optimization is critical in Computed Tomography (CT) for achieving desired diagnostic image quality while minimizing radiation dose. Due to the inter-effect of influencing CT parameters, traditional optimization methods rely on the testing of exhaustive combinations of these parameters. This poses a notable limitation due to the impracticality of exhaustive parameter testing. This study introduces a novel methodology leveraging Virtual Imaging Trials (VITs) and reinforcement learning to more efficiently optimize CT protocols. Computational phantoms with liver lesions were imaged using a validated CT simulator and reconstructed with a novel CT reconstruction Toolkit. The optimization parameter space included tube voltage, tube current, reconstruction kernel, slice thickness, and pixel size. The optimization process was done using a Proximal Policy Optimization (PPO) agent which was trained to maximize the Detectability Index (d’) of the liver lesion for each reconstructed image. Results showed that our reinforcement learning approach found the absolute maximum d’ across the test cases while requiring 79.7% fewer steps compared to an exhaustive search, demonstrating both accuracy and computational efficiency, offering a efficient and robust framework for CT protocol optimization. The flexibility of the proposed technique allows for use of varying image quality metrics as the objective metric to maximize for. Our findings highlight the advantages of combining VIT and reinforcement learning for CT protocol management.

Fenwick, David [Duke University Medical Center]↗
DOE OSTI2025

Field expedient stool collection methods for gut microbiome analysis in deployed military environments

ABSTRACT Field expedient devices and protocols for the collection, storage, and shipment of stool samples in deployed settings are needed for the advancement of microbiome research in military health. Relevant assessments include the evaluation of microbiome signatures associated with susceptibility to travelers’ diarrhea and recovery of gut function following infection. However, inherent biases in microbial measurements due to preservatives and sampling methods are unclear and should be assessed for an accurate evaluation of the microbiome. We performed shotgun metagenomic sequencing and compared the microbiome composition in paired fecal samples collected using Flinters Technology Associates (FTA) cards and OMNIgene (OG) Gut tubes, prior to and during international travel, from 49 adult participants, 39 of whom remained asymptomatic and 10 experienced travelers’ diarrhea. Higher concentrations of nucleic acid and sequencing libraries were observed in OG samples. A majority of genera (82.9%) were detected with both methods, and detections of genera limited to one collection method were not highly prevalent across samples and were present in extremely low relative abundances (<0.01%). Differences in beta diversity were largely explained by inter-individuality of microbiome composition, followed by the effect of collection method and timepoint-disease states. Differential abundance analysis indicated that Corynebacterium and Blautia were consistently higher in abundance across all groups with FTA and OG collection, respectively. The observed differences in microbiome composition between methods suggest the need for consistent and standardized protocols within a study. Overall, the data presented here could help guide the future design of fecal microbiome study protocols in field and military deployment settings. IMPORTANCE The assessment of field-deployable methods for fecal sample collection and storage is required to reliably capture samples collected in remote and austere locations. This study describes a comparative metagenomics analysis between samples collected by two different commercially available methods in a military-deployed setting. The results presented here are foundational for the future design of fecal microbiome study protocols in an operational context.

field study↗
DOE OSTI2026

Experimental Benchmarking for High-Reproducibility, Cross-Institutional Evaluation of Iron Redox Electrochemistry

We present a practical case study standardizing experimental protocols between collaborators with the goal of understanding ferrous iron (Fe 2+ ) chemistry and improving the iron deposition reaction for energy-efficient, electrochemical iron production. The study of iron reactions can be difficult, as aqueous iron electrolytes exhibit complex behaviors that can lead to differing interpretation of ostensibly similar experiments. The question we want to answer: are we studying the same chemistry? Our protocols address inherent challenges such as the tendency for Fe 2+ to spontaneously oxidize to ferric iron (Fe 3+ ) and the production of hydrogen at the potentials of interest. Our standardized protocol, executed by four collaborators in different labs and institutions, yields high-reproducibility results, and identified glassy carbon electrode surface quality and Fe 3+ impurities in the salt as key factors with outsized effects on cyclic voltammetry measurements. The process of developing the protocols helped to troubleshoot underlying issues that created poorer reversibility and reproducibility. This study highlights the fact that even nominally straightforward electrochemical systems can yield vastly different outcomes due to small differences in experimental preparation and serves as a useful example for creating transparent and achievable standards for the generation of reliable datasets that can be widely used and shared.

Ketter, Benjamin [Argonne National Laboratory (ANL↗
DOE OSTI2026

Data for "Genetics of flooding tolerance in an F2 Miscanthus sacchariflorus ssp. lutarioriparius × M. sinensis population"

This dataset contains all data and supplementary materials from "Genetics of flooding tolerance in an F2 Miscanthus sacchariflorus ssp. lutarioriparius × M. sinensis population". 1. The dataset S1 table contains the raw phenotypic data collected during the experiment. 2. The dataset S2 table contains the LSmean values for the 24 traits studied. 3. The dataset S3 table contains the TASSEL GBSv2 map, marker information, and genotype data used for mapping. 4. The dataset S4 table contains information on candidate genes found in each of the QTL intervals. 5. The dataset S5 table contains the GO annotations and KEGG enrichment analyses for those candidate genes. 6. The dataset S6 table contains information on the sequences used to classify AP2 ERF transcription factors. 7. The dataset S7 table contains information on AP2 ERF orthologs between Miscanthus and rice based on synteny. 8. Supplementary file 1 contains the ANOVA results using the raw phenotypic data collected from protocol "A". 9. Supplementary file 2 contains the ANOVA results using the raw phenotypic data collected from protocol "B". 10. Supplementary file 3 contains notes on the comparison of SNP calling methods. 11. Supplementary file 4 is a script for analyzing candidate genes found in QTL intervals.

Miscanthus, flood, partial submergence, complete s↗
DOE OSTI2026

CHESS 2025: Leaf Area Index (LAI) for meadow, shrub, tree, and understory vegetation

This dataset contains Leaf Area Index (LAI) measurements made as part of the Colorado Headwaters Ecological Spectroscopy Study (CHESS) during June and July of 2025. Data were collected in the Upper Gunnison Basin, Colorado, across three study domains: the Upper East River (CRBU), Almont Triangle (ALMO), and the Upper Taylor Basin (UPTA). Field observations of LAI were collected within 72 hours of airborne data collection by the National Ecological Observatory Network’s Aerial Observation Platform (NEON AOP). The NEON AOP collected waveform LiDAR (Light Detection and Ranging) and imaging spectrometer data in 426 spectral bands from the visible to shortwave infrared. LAI measurements were collected using the LICOR LAI-2200C Plant Canopy Analyzer following protocols outlined in the instrument manual (LI-COR 2019). Sampling targeted four distinct vegetation types: meadows, shrubs, trees, and aspen forest understory. We have archived data separately by site type because different field methods were used for each. At meadow sites, measurements were made at the four corners of 1m x 1m plots, with the instrument moving inward toward the center of the plot. At shrub sites, we measured the canopies of individual shrubs. At tree sites, we made measurements within a 10m x 10m subplot centered around a focal tree, with 30 observations taken on a regular grid. At aspen understory sites, we measured overstory trees following the tree protocol and understory herbaceous vegetation following the meadow protocol. All measurements included above-canopy (A) and below-canopy (B) readings, with specific protocols for scattering correction measurements in direct-sun conditions. Data were processed using the R package `rlai` (Worsham 2025). This package includes functions to calculate LAI, gap fraction, apparent clumping factor (Ω), scattering correction, and other canopy metrics. Package contents: Full file descriptions appear in ‘flmd.csv’. Files named according to the convention ‘lai_*_summary_data_cleaned.csv’ contain summary values of LAI, apparent clumping factor (Ωapp), and scattering correction factors for each site. These are the analysis-ready products that most data users will work with. Files named ‘lai_*_metadata_cleaned.csv’ contain additional site-level observations made during field collection. We have also archived intermediate and supplementary data for users who wish to check our processing approach or apply alternative methods. ‘raw_lai_2200C.zip’ contains the raw files as read from the LI-COR instrument, with no processing applied, in TXT format. The zip archive contains subdirectories by site type, which are further subdivided by sampling area. Filenames correspond to the sampling site number. ‘intermediate_results.zip’ contains detailed output from the processing routines, in JSON format. The zip archive contains subdirectories by site type; filenames correspond to the sampling site number. ‘scattering_correction_logs.zip’ contains logfiles from the implementation of Kobayashi et al.'s (2013) scattering correction algorithm. The logfiles report values of several parameters at each iteration of the algorithm, as the model converges toward a stable solution. They are intended for users who want to verify scattering correction performance. The zip archive contains subdirectories by site type; filenames correspond to the sampling site number. ‘spot_checks.csv’ reports LAI and other values for a small number of files processed with LI-COR FV2200 software (LI-COR 2013) using the same control parameters as in our R-based approach. Additional metadata are provided in a data dictionary describing column names and definitions (dd.csv), and in a file-level metadata file (flmd.csv). All zip files can be expanded with common archive utilities. TXT, CSV, and JSON files can be ingested into R or Python computing environments or read in common text editor utilities. Geospatial information: Geospatial data for mapping measurement site locations are in the files CHESS_polygons_lai_UTM.geojson, CHESS_polygons_shrub_UTM.geojson, and CHESS_polygons_meadow_UTM.geojson in the companion geospatial package for the 2025 CHESS campaign, ‘CHESS 2025: Location data for field observations and sampling’ (Henderson et al., 2026). CHESS Project Description: The Colorado Headwaters Ecological Spectroscopy Study (CHESS) comprised a multi-week airborne remote sensing and field observation campaign in the Upper Gunnison Basin, Colorado, conducted in June and July of 2025. Airborne remote sensing was conducted by the National Ecological Observatory Network Airborne Observation Platform (NEON AOP), concurrent with a field campaign run by the Rocky Mountain Biological Laboratory (RMBL), the Lawrence Berkeley National Laboratory (LBNL) and SLAC National Accelerator Laboratory Watershed Function Science Focus Area (SFA), and NASA-JPL (Jet Propulsion Laboratory) Earth Surface Mineral Dust Source Investigation (EMIT) program. Between June 10 and July 18, 2025, the NEON AOP flight team collected high-resolution aerial imaging spectroscopy and Light Detection and Ranging (LiDAR) data over three domains: the Upper East River (CRBU), Almont Triangle (ALMO), and the Upper Taylor Basin (UPTA). In coordination with the flights, a field campaign acquired ground-truth observations, including observations of vegetation composition, foliar traits, forest demography, and subsurface properties in 18 core sampling areas within the domains. Additional surface water observations were taken at over 380 point locations. All CHESS campaign datasets can be found within the CHESS ESS-DIVE data portal: https://data.ess-dive.lbl.gov/portals/chess. Funding Acknowledgement: Field and remote-sensing data acquisition was performed under a grant from the National Aeronautics and Space Administration (80NSSC24K1005). This work was also supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231. * Todorov and Worsham are co–first authors.

2018 NEON and 2025 CHESS Campaigns↗
DOE OSTI2025

Multiqubit entanglement generation with squeezed modes

We present a hybrid continuous variable–discrete variable entanglement generation protocol using linear optics and homodyne measurements, capable of producing multiple high-fidelity Bell pairs per protocol iteration, with an approximate 0.5 success probability. The effectiveness of the protocol is determined by the squeezing strength. To increase the number of Bell pairs, approximately 3 dB of extra squeezing is needed for each additional Bell pair. The protocol also generates single Bell pairs with an approximate 0.75 probability for squeezing strengths ⪅ 15 dB , achievable with current technology.

Macridin, Alexandru [Fermilab] (ORCID:000000022228↗
NASA NTRS1984

ARQ schemes for data transmission in mobile radio systems

The problem of providing data communications over a land mobile radio channel is considered, taking into account error detection combined with retransmission on request as a means of obtaining reliability in digital data transmission. The considered systems are referred to as automatic RQ (repeat-request) or ARQ. It is pointed out that the distinguishing features of a land mobile radio (LMR) system, as far as the ARQ protocol is concerned, are a frequency or pair of frequencies shared by a large population of half-duplex users which cannot transmit and receive simultaneously. A description is presented of those variations of ARQ which are adaptable to an LMR system. A new channel model for a fading LMR channel is developed, and several ARQ protocols and some new protocol combinations are described. The relationships are developed for a description of the various protocols in terms of channel failure probabilities. Undetected error probabilities are developed and compared.

Comroe, R. A.↗
NASA NTRS1985

Verification and performance tests of HYCAR program

The HYCAR program simulates the network protocols of HYPERchannel and Fiber Optic Demonstration System (FODS) and other related protocols. Verification tests of the program were conducted using the FODS protocol. The tests validated the operation of the program through deterministic and analytical means. Extensive experimentation with the simulator produced a set of performance characteristics for the FODS protocol under varied loading conditions. These characteristics are consistent with those expected, and are documented with the validation tests.

Bhatia, Veena↗
NASA NTRS1990

Communication-Gateway Software For NETEX, DECnet, And TCP/IP

Communications gateway software, GATEWAY, provides process-to-process communication between remote applications programs in different protocol domains. Communicating peer processes may be resident on any paired combination of NETEX, DECnet, or TCP/IP hosts. Provides necessary mapping from one protocol to another and facilitates practical intermachine communications in cost-effective manner by eliminating need to standardize on single protocol or to implement multiple protocols in host computers. Written in Ada.

Keith, B.↗
NASA NTRS1990

Extremely high data-rate, reliable network systems research

Significant progress was made over the year in the four focus areas of this research group: gigabit protocols, extensions of metropolitan protocols, parallel protocols, and distributed simulations. Two activities, a network management tool and the Carrier Sensed Multiple Access Collision Detection (CSMA/CD) protocol, have developed to the point that a patent is being applied for in the next year; a tool set for distributed simulation using the language SIMSCRIPT also has commercial potential and is to be further refined. The year's results for each of these areas are summarized and next year's activities are described.

Foudriat, E. C.↗
NASA NTRS1990

Traffic placement policies for a multi-band network

Recently protocols were introduced that enable the integration of synchronous traffic (voice or video) and asynchronous traffic (data) and extend the size of local area networks without loss in speed or capacity. One of these is DRAMA, a multiband protocol based on broadband technology. It provides dynamic allocation of bandwidth among clusters of nodes in the total network. A number of traffic placement policies for such networks are proposed and evaluated. Metrics used for performance evaluation include average network access delay, degree of fairness of access among the nodes, and network throughput. The feasibility of the DRAMA protocol is established through simulation studies. DRAMA provides effective integration of synchronous and asychronous traffic due to its ability to separate traffic types. Under the suggested traffic placement policies, the DRAMA protocol is shown to handle diverse loads, mixes of traffic types, and numbers of nodes, as well as modifications to the network structure and momentary traffic overloads.

Maly, Kurt J.↗
NASA NTRS1989

Editing wild points in isolation - Fast agreement for reliable systems (Preliminary version)

Consideration is given to the intuitively appealing notion of discarding sensor values which are strongly suspected of being erroneous in a modified approximate agreement protocol. Approximate agreement with editing imposes a time bound upon the convergence of the protocol - no such bound was possible for the original approximate agreement protocol. This new approach is potentially useful in the construction of asynchronous fault tolerant systems. The main result is that a wild-point replacement technique called t-worst editing can be shown to guarantee convergence of the approximate agreement protocol to a valid agreement value. Results are presented for a four-processor synchronous system in which a single processor may be faulty.

Kearns, Phil↗
NASA NTRS1990

Issues in designing transport layer multicast facilities

Multicasting denotes a facility in a communications system for providing efficient delivery from a message's source to some well-defined set of locations using a single logical address. While modem network hardware supports multidestination delivery, first generation Transport Layer protocols (e.g., the DoD Transmission Control Protocol (TCP) (15) and ISO TP-4 (41)) did not anticipate the changes over the past decade in underlying network hardware, transmission speeds, and communication patterns that have enabled and driven the interest in reliable multicast. Much recent research has focused on integrating the underlying hardware multicast capability with the reliable services of Transport Layer protocols. Here, we explore the communication issues surrounding the design of such a reliable multicast mechanism. Approaches and solutions from the literature are discussed, and four experimental Transport Layer protocols that incorporate reliable multicast are examined.

Dempsey, Bert J.↗
NASA NTRS1993

Reliable transfer of data from ground to space

This paper describes the problems involved in uplink of data from control centers on the ground to spacecraft, and explores the solutions to those problems, past. present. and future. The evolution of this process, from simple commanding to transfer of large volumes of data and commands is traced. The need for reliable end-to-end protocols for commanding and file transfer is demonstrated, and the shortcomings of both existing telecommand protocols and commercial products to meet this need are discussed. Recent developments in commercial protocols that may be adaptable to the mentioned operations environment are surveyed, and current efforts to develop a suite of protocols for reliable transfer in this environment are presented.

Brosi, Fred↗
NASA NTRS1992

The effect of intermittent standing or walking during head down tilt bedrest on peak O2 consumption

The cardiovascular aspect of bedrest deconditioning is manifested by decreases in peak O2 uptake (VO(sub 2 peak)) during minimal exercise. The effect of intermittent standing (+G(z)) or walking (+G(z)W) during 4 days of 7 degree Head Down Tilt bedrest (HDT) on VO(sub 2 peak) was evaluated. Methods: Five protocols were performed by eight male subjects; control (C) consisting of complete bedrest, and 15 minute periods to total 2 or 4 hours daily of standing (+G(z)(exp 2) and +G(z)(exp 4) respectively) or walking at 3.0 MPH (+G(z)W2 and +G(z)W4 respectively). Subjects performed VO(sub 2 peak) tests prior to and on the final day of HDT. VO(sub 2 peak) was determined using open circuit indirect calorimetry during supine leg cycling ergometry. After a 5 minute warmup, three 2 minute incremental loads of 33 W previously determined to elicit VO(sub 2 peak) were given and the subject cycled to volitional fatigue. Results: The C protocol VO(sub 2 peak) decreased by 16 percent (2.71 plus or minus 0.16 to 2.27 plus or minus 0.14 L/min) and 11 percent in +G(z)(exp 4) (2.72 plus or minus 0.15 to 2.43 plus or minus 0.14 L/min). With +G(z)W2 VO(sub 2 peak) decreased by 9 percent (2.71 plus or minus 0.17 to 2.46 plus or minus 0.14 L/min) and with +G(z)W4, VO(sub 2 peak) decreased by 10 percent (2.71 plus or minus 0.14 to 2.43 plus or minus 0.14 L/min). VO(sub 2 peak) in all protocols decreased with HDT (P less than 0.05). The decrease in C VO(sub 2 peak) was significantly greater (P less than 0.05) than the decreases in either +G(z) or +G(z)W protocols. Conclusion: The deconditioning that occurs after only 4 days of HDT was demonstrated by decreases in VO(sub 2 peak). Intermittent +G(z) or +G(z)W attenuated, but did not prevent, the decrease in VO(sub 2 peak) with HDT.

Ertl, A. C.↗
NASA NTRS1992

The efficacy of periodic +Gz exposure in the prevention of bedrest induced orthostatic intolerance

What is the most efficient dosage of periodic exposure to positive 1G(z) during microgravity to maintain a functional upright position after returning to a positive 1G(z) environment? The answer has implications for the type of countermeasures astronauts will be required to perform during long term space flight. Methods: Nine males were subjected to four different positive 1G exposure protocols plus a control protocol ('zero G(z)') during four days of continuous bedrest. The four positive 1G(z) exposures consisted of periodic standing or walking, each for a total period of two or four hours. Each subject was returned for bedrest on five different occasions over a period of approximately one year to obtain data on each of the nine subjects across all four positive 1G(z) treatments and the control. A 30 min tilt test was used to measure orthostatic response during pre and post bedrest. Results: In terms of survival rate (percentage of subjects who did not faint after 30 sec of tilt), four hours of intermittent standing was the only protocol that maintained a rate comparable to pre bedrest levels (87.5 percent). Although the other three positive 1G(z) protocols performed better than the 'zero G(z) control (22.2 percent), only the four hour standing returned post bedrest survival rates to pre bedrest levels. Conclusions: The results will need to be evaluated with regards to a variety of other physiological systems which are known to decondition during microgravitry.

Ludwig, D. A.↗
NASA NTRS1994

Experimental control in software reliability certification

There is growing interest in software 'certification', i.e., confirmation that software has performed satisfactorily under a defined certification protocol. Regulatory agencies, customers, and prospective reusers all want assurance that a defined product standard has been met. In other industries, products are typically certified under protocols in which random samples of the product are drawn, tests characteristic of operational use are applied, analytical or statistical inferences are made, and products meeting a standard are 'certified' as fit for use. A warranty statement is often issued upon satisfactory completion of a certification protocol. This paper outlines specific engineering practices that must be used to preserve the validity of the statistical certification testing protocol. The assumptions associated with a statistical experiment are given, and their implications for statistical testing of software are described.

Trammell, Carmen J.↗
NASA NTRS1995

Network time synchronization servers at the US Naval Observatory

Responding to an increased demand for reliable, accurate time on the Internet and Milnet, the U.S. Naval Observatory Time Service has established the network time servers, tick.usno.navy.mil and tock.usno.navy.mil. The system clocks of these HP9000/747i industrial work stations are synchronized to within a few tens of microseconds of USNO Master Clock 2 using VMEbus IRIG-B interfaces. Redundant time code is available from a VMEbus GPS receiver. UTC(USNO) is provided over the network via a number of protocols, including the Network Time Protocol (NTP) (DARPA Network Working Group Report RFC-1305), the Daytime Protocol (RFC-867), and the Time protocol (RFC-868). Access to USNO network time services is presently open and unrestricted. An overview of USNO time services and results of LAN and WAN time synchronization tests will be presented.

Schmidt, Richard E.↗