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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 793 records · Page 44
All sourcesNASA NTRSDOE OSTI
DOE OSTI2012

AvrRpm1 Missense Mutations Weakly Activate RPS2-Mediated Immune Response in Arabidopsis thaliana

Plants recognize microbes via specific pattern recognition receptors that are activated by microbe-associated molecular patterns (MAMPs), resulting in MAMP-triggered immunity (MTI). Successful pathogens bypass MTI in genetically diverse hosts via deployment of effectors (virulence factors) that inhibit MTI responses, leading to pathogen proliferation. Plant pathogenic bacteria like Pseudomonas syringae utilize a type III secretion system to deliver effectors into cells. These effectors can contribute to pathogen virulence or elicit disease resistance, depending upon the host plant genotype. In disease resistant genotypes, intracellular immune receptors, typically belonging to the nucleotide binding leucine-rich repeat family of proteins, perceive bacterial effector(s) and initiate downstream defense responses (effector triggered immunity) that include the hypersensitive response, and transcriptional re-programming leading to various cellular outputs that collectively halt pathogen growth. Nucleotide binding leucine-rich repeat sensors can be indirectly activated via perturbation of a host protein acting as an effector target. AvrRpm1 is a P. syringae type III effector. Upon secretion into the host cell, AvrRpm1 is acylated by host enzymes and directed to the plasma membrane, where it contributes to virulence. This is correlated with phosphorylation of Arabidopsis RIN4 in vivo. RIN4 is a negative regulator of MAMP-triggered immunity, and its modification in the presence of four diverse type III effectors, including AvrRpm1, likely enhances this RIN4 regulatory function. The RPM1 nucleotide binding leucine-rich repeat sensor perceives RIN4 perturbation in disease resistant plants, leading to a successful immune response. Here, demonstrate that AvrRpm1 has a fold homologous to the catalytic domain of poly(ADP-ribosyl)polymerase. Site-directed mutagenesis of each residue in the putative catalytic triad, His63-Tyr122-Asp185 of AvrRpm1 results in loss of both AvrRpm1-dependent virulence and AvrRpm1-mediated activation of RPM1, but, surprisingly, causes a gain of function: the ability to activate the RPS2 nucleotide binding leucine-rich repeat sensor.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2017

Final Technical Report: Targeting DOE-Relevant Ions with Supramolecular Strategies, DE-SC0010555

The effectiveness of three popular supramolecular strategies to selectively target negatively charged ions (anions) was evaluated. Ions of interest included oxo anions, particularly sulfate, that hamper nuclear waste remediation. Three objectives were pursued using a simple building block strategies and by strategically placing anion-binding sites at appropriate positions on organic host molecules. The goal of the first objective was to assess the influence of secondary, tertiary and quaternized amines on binding tetrahedral anions using mixed amide/amine macrocyclic and urea/amine hosts containing aromatic or heteroaromatic spacers. Objective 2 focused on the design of ion pair hosts, using mixed macrocyclic anion hosts joined through polyether linkages. Objective 3 was to explore the synthesis of new metal-linked extended macrocyclic frameworks to leverage anion binding. Key findings were that smaller 24-membered macrocycles provided the most complementary binding for sulfate ion and mixed urea/amine chelates showed enhanced binding over amide corollaries in addition to being highly selective for SO 4 2- in the presence of small quantities of water. In addition to obtaining prototype metal-linked macrocyclic anion hosts, a new dipincer ligand was designed that can be used to link macrocyclic or other supramolecular hosts in extended frameworks. When the tetraamide-based pincers are bound to two metal ions, an interesting phenomenon occurs. Upon deprotonation of the amides, two new protons appear between adjacent carbonyl pairs on the ligand, which may modify the chemistry, and metal-metal interactions in the complexes. Gel formation occurred for some of these extended hosts, and the physical properties are currently under investigation. The new tetracarboxamide-based pincers can also provide basic frameworks for double macrocycles capable of binding ion pairs as well as for binding metal ions and exploring intermetallic interactions through the pyrazine π system. Additionally appendages capable of influencing solvation effects can be introduced, and a number of other potential applications can be realized in areas such as soft materials chemistry, catalysis, sensing, and proton switches, the latter for binding and release of targeted guests. These findings provide a better foundation for understanding the selective binding of anions by targeted placement of hydrogen binding sites, and the strengths and weaknesses of various functional groups, that will allow for more the design of more effective anion sequestering agents. Our design strategy also used simple, cost-effective building blocks for host synthesis to allow for scale-up should real-world applications be forthcoming.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2019

Broadband Outdoor Radiometer Calibration (BORCAL) Process for the Atmospheric Radiation Measurement (ARM) Program: Second Edition

The Atmospheric Radiation Measurement program (ARM) maintains a fleet of monitoring stations to aid in the improved scientific understanding of the basic physics related to radiative feedback processes in the atmosphere, particularly the interactions among clouds and aerosols. ARM obtains continuous measurements and conducts field campaigns to provide data products that aid in the improvement and further development of climate models. All of the measurement campaigns include a suite of solar measurements. The Solar Radiation Research Laboratory at the National Renewable Energy Laboratory supports ARM's full suite of stations in a number of ways, including troubleshooting issues that arise as part of the data-quality reviews; managing engineering changes to the standard setup; and providing calibration services and assistance to the full fleet of solar-related instruments, including pyranometers, pyrgeometers, pyrheliometers, as well as the temperature/relative humidity probes, multimeters, and data acquisition systems that are used in the calibrations performed at the Southern Great Plains Radiometer Calibration Facility. This paper discusses all aspects related to the support provided to the calibration of the instruments in the solar monitoring fleet.

14 SOLAR ENERGY↗
DOE OSTI2021

Structural analyses of macromolecules by solution scattering (CRADA Final Report)

New innovative Small Angle X-ray Scattering (SAXS) methods to visualized macromolecules in solution at low resolution has led the SIBYLS group at Lawrence Berkeley Lab in being a world leader in biological SAXS. This research is particularly important now as LBNL establishes high-priority research topics including molecular to mesoscale analysis. This new expanding area of research relies on and ties in with our efforts towards developing a solution structure modeling tool for RNA structure prediction. Due to the pandemic our efforts focused on the SARS-CoV-2 proteins and RNA interactions. Specifically, we characterized SARS-CoC-2 proteins interaction with RNA (Wilanowski et al. 2021 -Hammel group) and Nucleoprotein interaction with RNA (Schneidman group).

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2019

Identification of plant host and microbe model system for study of rhizophagy cycle

Rhizophagy (“root eating”) is a process by which plants actively uptake soil microbes into their roots and use oxidative degradation to extract micronutrients from the cells. This phenomenon has been proposed to be a general mechanism for plants to acquire nutrients from the environment via bacteria and fungi. Determining the conditions under which rhizophagy occurs and its prevalence in different plant species will improve our understanding of nutrient dynamics and plant-microbe metabolic interactions at the molecular level. This project aims to (1) optimize methods for plant and microbial growth, imaging, and analysis for the study of rhizophagy and (2) identify a bioenergy-relevant model system for future rhizophagy studies.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Elucidating Photoinduced Processes of Photosystem I Via Multidimensional Electronic and Vibrational Spectroscopies

This project was motivated by an overarching goal to elucidate the mechanism of energy and electron transfer that governs the efficient charge separation in photosystem I (PSI) complexes. PSI is a natural light harvesting complex that drives oxygenic photosynthesis in plants, algae, and cyanobacteria. It uses ~300 tightly packed chlorophylls (Chls) to absorb photons, transfer the excitation energy to the reaction center (RC), and generate a charge separated state with near unity quantum efficiency (QE). A better understanding of the mechanism of energy transfer and charge separation in PSI is required for understanding the high QE of natural light harvesting complexes, and it could lead to the further development of artificial photosynthetic systems for solar energy conversion and modification of light harvesting complexes to improve crop yields. We applied two-dimensional optical spectroscopies to different cyanobacterial photosystem I complexes, including PSI complexes that contain Chl f molecules, to map energy transfer pathways and gain insight into the efficient light harvesting of PSI. We used two-dimensional electronic spectroscopies (2DES) to map energy transfer in Chl a and Chl f containing PSI complexes. To investigate the Chl f PSI complexes, we modified our spectrometer to probe the lower energy states associated with Chl f molecules. We interpreted the 2DES spectra through global analysis procedures to generate maps of energy transfer. We also constructed a two-dimensional electronic vibrational (2DEV) spectrometer that will be used to investigate charge transfer transitions and dynamics within PSI complexes. Measurements were performed on model systems to establish general data analysis procedures for interpreting 2D spectra and gain insight into protein cofactor interactions.

14 SOLAR ENERGY↗
DOE OSTI2020

A Guide to Conquer the Biological Network Era Using Graph Theory

Networks are one of the most common ways to represent biological systems as complex sets of binary interactions or relations between different bioentities. In this article, we discuss the basic graph theory concepts and the various graph types, as well as the available data structures for storing and reading graphs. In addition, we describe several network properties and we highlight some of the widely used network topological features. We briefly mention the network patterns, motifs and models, and we further comment on the types of biological and biomedical networks along with their corresponding computer- and human-readable file formats. Finally, we discuss a variety of algorithms and metrics for network analyses regarding graph drawing, clustering, visualization, link prediction, perturbation, and network alignment as well as the current state-of-the-art tools. We expect this review to reach a very broad spectrum of readers varying from experts to beginners while encouraging them to enhance the field further.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2017

Theoretical Models of Optical Transients. I. A Broad Exploration of the Duration–Luminosity Phase Space

The duration–luminosity phase space (DLPS) of optical transients is used, mostly heuristically, to compare various classes of transient events, to explore the origin of new transients, and to influence optical survey observing strategies. For example, several observational searches have been guided by intriguing voids and gaps in this phase space. However, we should ask, do we expect to find transients in these voids given our understanding of the various heating sources operating in astrophysical transients? In this work, we explore a broad range of theoretical models and empirical relations to generate optical light curves and to populate the DLPS. We explore transients powered by adiabatic expansion, radioactive decay, magnetar spin-down, and circumstellar interaction. For each heating source, we provide a concise summary of the basic physical processes, a physically motivated choice of model parameter ranges, an overall summary of the resulting light curves and their occupied range in the DLPS, and how the various model input parameters affect the light curves. We specifically explore the key voids discussed in the literature: the intermediate-luminosity gap between classical novae and supernovae, and short-duration transients (≲10 days). We find that few physical models lead to transients that occupy these voids. Moreover, we find that only relativistic expansion can produce fast and luminous transients, while for all other heating sources events with durations ≲10 days are dim (M{sub R}≳−15 mag). Finally, we explore the detection potential of optical surveys (e.g., Large Synoptic Survey Telescope) in the DLPS and quantify the notion that short-duration and dim transients are exponentially more difficult to discover in untargeted surveys.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS1972

Hypersonic shock-interaction phenomena applicable to space shuttle configurations

The convective heat transfer distribution for space shuttle configurations is discussed. The viscous/inviscid interactions associated with the complex three dimensional flow fields are examined. Two basic conditions are considered as follows: (1) models consisting of basic elemental combinations and (2) models of specific flight vehicles. The test facilities and test programs used to obtain data on the fuselage flow field and the wing flow field are described.

Bertin, J. J.↗
NASA NTRS1973

Photoheliograph thermal concepts study. Volume 1: Summary

The performances of thermal subsystem concepts for each of three photoheliograph designs oriented toward unique solar observation missions are defined. These designs and missions may be categorized as: (1) A 150-centimeter photoheliograph for large Solar Observatory (LSO) missions; (2) a 100-centimeter photoheliograph for shuttle sorties; and (3) a 100-centimeter photoheliograph for balloon missions. For each of these photoheliograph designs are the basic thermal environments, boundary conditions, and applicable thermal control concepts defined, the interactions between concepts and environments determined, and a baseline thermal control concept for the three classes of photoheliographs selected. A set of experimental work plans describing the major efforts required to further the detailed design and to confirm analytical performance predictions is included.

Source record↗
NASA NTRS1975

Soil moisture: Some fundamentals

A brief tutorial on soil moisture, as it applies to agriculture, is presented. Information was taken from books and papers considered freshman college level material, and is an attempt to briefly present the basic concept of soil moisture and a minimal understanding of how water interacts with soil.

Milstead, B. W.↗
NASA NTRS1978

Up-date of traveling wave tube improvements

NASA research in the area of traveling wave tube technology is reviewed, with emphasis on the basic physics of guns and collectors and a computer model for the interaction between the electron beam and the RF circuit. The design of a multistage depressed collector, capable of multiplying tube efficiency by a factor of two or more, is presented; one such design has been adopted for commercial traveling wave tube production. A three-dimensional model of electron trajectories toward the collector also receives attention, as does the problem of RF circuit losses.

Buck, E.↗
NASA NTRS1978

Analytical study of STOL Aircraft in ground effect. Part 2: Nonplanar, nonlinear method applicable to three dimensional jets of finite thickness

The ability of the potential flow analysis (POTFAN) to predict the influence of ground proximity on lift systems is examined. A two dimensional study employing vortex lattice methodology provides confidence that ground effect phenomenon can be predicted using discrete singularity representation. Two dimensional quasi-steady ascent and descent behavior determined provides guidance in interpreting three dimensional results. Steady and quasi-steady ground effect aerodynamic characteristics predicted by POTFAN are presented for several basic unpowered configurations. POTFAN results are compared with experimental data and results of other analytical methods. Modification of POTFAN to incorporate multienergy flow analysis is discussed. General aspects of thick jet models are examined to provide a basic for extending POTFAN's scope to include analysis of propulsive lift interactions.

Shollenberger, C. A.↗
NASA NTRS1986

An ab initio investigation of possible intermediates in the reaction of the hydroxyl and hydroperoxyl radicals

Ab initio quantum chemical techniques have been used to investigate covalently-bonded and hydrogen-bonded species that may be important intermediates in the reaction of hydroxyl and hydroperoxyl radicals. Stable structures of both types were identified. Basic sets of polarized double-zeta quality and large scale configuration interaction wave functions have been utilized. Based upon electronic energies, the covalently-bonded HOOOH species is found to be 26.4 kcal/mol more stable than the OH and HO2 radicals. Similarly, the hydrogen-bonded HO-HO2 species is found to have an electronic energy 4.7 kcal/mol below that of the component radicals, after correction is made for the basis set superposition error. The hydrogen-bonded form is found to be planar, to possess one relatively 'normal' hydrogen bond, and to have lowest energy 3A-prime and 1A-prime states that are essentially degenerate. The 1A-double prime and 3A-double prime excited states produced by rotation of the unpaired OH electron into the molecular plane are found to be very slightly bound.

Jackels, C. F.↗
NASA NTRS1993

Environmental influences on galaxy evolution

We investigate the role of mergers and interactions in the evolution of galaxies by studying galaxies in compact groups. Compact groups of galaxies have high spatial densities and low velocity dispersions making these regions ideal laboratories in which to study the effect of interactions and mergers. Based on a detailed spectroscopic and multi-color imaging study, we find that both the isophotal shapes and the stellar kinematics indicate that many of the elliptical galaxies in compact groups have been affected by tidal interactions. At the same time, however, we find that only a few elliptical galaxies in compact groups have evidence for the young stellar populations that would be expected if they are the result of recent merger of two spiral galaxies. Therefore, we conclude that tidal interactions affect galaxy properties at the current epoch, but the bulk of basic galaxy formation and transformation must have occurred at much higher redshift.

Zepf, Stephen E.↗
NASA NTRS1993

The MRIS feasibility study

The Microwave Reflectometer Ionization Sensor (MRIS) is an instrument being developed for use in detecting and ranging of electron density layers in the reentry plasma of a space transfer vehicle. The rationale for the selection of the Double Sideband Suppressed Carrier (DSBSC) system used in the feasibility study for the MRIS is presented. A 25 GHz single-oscillator system and a 220 GHz double-oscillator system are described. The 25 GHz system was constructed and tested in the laboratory and test results are presented. As developed, the system employs a sideband spacing of 160 MHz. Based on an estimated electromagnetic wave velocity in the plasma, a round-trip phase shift measurement accuracy of +/- 7.6 degrees was required for the desired +/- 1/2 cm distance measurement accuracy. The interaction of parallel ground and reflecting planes produces interference that prevents the basic DSBSC system from meeting the accuracy goal so a frequency modulation was added to the system to allow averaging of the measured phase deviation. With an FM deviation of +/- 1 GHz, laboratory measurements were made for distances from 5 to 61 cm tip free space. Accounting for the plasma velocity factor, 82 percent of the data were equal to or better than the desired accuracy. Based on this measured result a sideband spacing to 250 MHz could be expected to yield data approximately 96 percent within the accuracy goal.

Neece, Robert T.↗
NASA NTRS1993

Global barotropic response to a tropical forcing

Zonally varying flow has been used to initialize numerical models and has been shown to play an important role in strong localized responses both in extratropics and the tropics. In this study, a climatological 200 mb January mean is used as a steady basic state of a barotropical model which consists of shallow water equations and a mass source centered at 4 deg S/120 deg E to simulate convective heating over Indonesia region. In the experiment, tropical responses appear not only over the western Pacific, where the forcing is located, but also over the eastern Pacific where the response is related to the zonally varying basic state. The westward propagating equatorial Rossby waves excited by the forcing interact with waves out of and into the tropics and the positive and negative phase of the Rossby waves result in blocking circulation over North America and tropical plumes over equatorial eastern Pacific, respectively.

Zhang, Yuxia↗