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At least 811 records · Page 45

Direct Numerical Simulation of Transition in a Swept-Wing Boundary Layer

Direct numerical simulation (DNS) is performed to examine laminar to turbulent transition due to high-frequency secondary instability of stationary crossflow vortices in a subsonic swept-wing boundary layer for a realistic natural-laminar-flow airfoil configuration. The secondary instability is introduced via inflow forcing derived from a two-dimensional, partial-differential-equation based eigenvalue computation; and the mode selected for forcing corresponds to the most amplified secondary instability mode which, in this case, derives a majority of its growth from energy production mechanisms associated with the wall-normal shear of the stationary basic state. Both the growth of the secondary instability wave and the resulting onset of laminar-turbulent transition are captured within the DNS computations. The growth of the secondary instability wave in the DNS solution compares well with linear secondary instability theory when the amplitude is small; the linear growth is followed by a region of reduced growth resulting from nonlinear effects before an explosive onset of laminar breakdown to turbulence. The peak fluctuations are concentrated near the boundary layer edge during the initial stage of transition, but rapidly propagates towards the surface during the process of laminar breakdown. Both time-averaged statistics and flow visualization based on the DNS reveal a sawtooth transition pattern that is analogous to previously documented surface flow visualizations of transition due to stationary crossflow instability. The memory of the stationary crossflow vortex is found to persist through the transition zone and well beyond the location of the maximum skin friction.

Duan, Lian↗

Molecular Genetics of Root Thigmoresponsiveness in Arabidopsis thaliana

The molecular mechanisms that allow plant roots to use gravity and touch as growth guides are investigated. We are using a molecular genetic strategy in Arabidopsis thaliana to study these processes. When Arabidopsis thaliana seedlings grow on tilted hard-agar surfaces, their roots develop a wavy pattern of growth which appears to derive from a succession of left-handed and right-handed circumnutation-like processes triggered by gravity and touch stimulation (Okada and Shimura, 1990; Rutherford et al., 1998; Rutherford and Masson, 1996). Interestingly, mutations that affect root waving on tilted hard-agar surfaces can be identified and characterized. Some of these mutations affect root gravitropism, while others appear to be responsible for the production of abnormal waves (no waves, compressed or square waves, coils) without affecting gravitropism. The specific objectives of this project were to functionally characterize two genes (WVD2 and WVD6) which are required for root waving on tilted agar surfaces, but not for root gravitropism. Specific objectives included a physiological and cytological analysis of the mutants, and molecular cloning and characterization of the corresponding genes. As summarized in this paper, we have reached these objectives. We have also identified and partially characterized other mutations that affect root skewing on hard-agar surfaces (sku5-1 and ago1), and have completed our work on the root-wave phenotype associated with mutations in genes of the tryptophan biosynthesis pathway (Lynn et al., 1999; Rutherford et al., 1998; Sedbrook et al., 2000, 2002). We briefly describe our progress on the cloning and characterization of WVD6, WVD2 and SKU5, and provide a list of papers (published, or in preparation) that derived from this grant. We also discuss the biological implications of our findings, with special emphasis on the analysis of WVD2.

Masson, Patrick H.↗

Copoly(Imide Siloxane) Abhesive Materials with Varied Siloxane Oligomer Length

Incorporation of PDMS moieties into a polyimide matrix lowered the surface energy resulting in enhanced adhesive interactions. Polyimide siloxane materials were generated using amine-terminated PDMS oligomers of different lengths to study changes in surface migration behavior, phase segregation, mechanical, thermal, and optical properties. These materials were characterized using contact angle goniometry, tensile testing, and differential scanning calorimetry. The surface migration behavior of the PDMS component depended upon the siloxane molecular weight as indicated by distinct relationships between PDMS chain length and advancing water contact angles. Similar correlations were observed for percent elongation values obtained from tensile testing, while the addition of PDMS reduced the modulus. High fidelity topographical modification via laser ablation patterning further reduced the polyimide siloxane surface energy. Initial particulate adhesion testing experiments demonstrated that polyimide siloxane materials exhibited greater abhesive interactions relative to their respective homopolyimides.

Wohl, Christopher J.↗

Spatial distributions of Tribrachidium, Rugoconites, and Obamus from the Ediacara Member (Rawnsley Quartzite), South Australia

The spatial distribution of in situ sessile organisms, including those from the fossil record, provides information about life histories, such as possible dispersal and/or settlement mechanisms, and how taxa interact with one another and their local environments. At Nilpena Ediacara National Park (NENP), South Australia, the exquisite preservation and excavation of 33 fossiliferous bedding planes from the Ediacara Member of the Rawnsley Quartzite reveals in situ communities of the Ediacara Biota. Here, the spatial distributions of three relatively common taxa, Tribrachidium, Rugoconites, and Obamus, occurring on excavated surfaces were analyzed using spatial point pattern analysis. Tribrachidium have a variable spatial distribution, implying that settlement or post-settlement conditions/preferences had an effect on populations. Rugoconites display aggregation, possibly related to their reproductive methods in combination with settlement location availability at the time of dispersal and/or settlement. Additionally, post-settlement environmental controls could have affected Rugoconites on other surfaces, resulting in lower populations and densities. Both Tribrachidium and Rugoconites also commonly occur as individuals or in low numbers on a number of beds, thus constraining possible reproductive strategies and environmental/substrate preferences. The distribution of Obamus is consistent with selective settlement, aggregating near conspecifics and on substrates of mature microbial mat. This dispersal process is the first example of substrate-selective dispersal among the Ediacara Biota, thus making Obamus similar to numerous modern sessile invertebrates with similar dispersal and settlement strategies. Resumen La distribución espacial de los organismos sésiles in situ, incluyendo los del registro fósil, brinda información sobre las historias de vida, tal como los posibles mecanismos de dispersión y/o asentamiento, y sobre cómo los taxones interactúan entre sí y entre sus entornos locales. En el Parque Nacional Nilpena Ediacara (NENP), Australia Meridional, la excelente preservación y excavación de 33 planos de lecho fosilífero del Miembro Ediacara de la Cuarcita Rawnsley revela comunidades in situ de la biota ediacárica. En este estudio analizamos las distribuciones espaciales de tres taxones relativamente comunes, Tribrachidium, Rugoconites y Obamus, que se encuentran en superficies excavadas mediante el análisis de patrones de puntos espaciales. Tribrachidium tiene una distribución espacial variable, lo que implica que las condiciones/preferencias durante o después del asentamiento tuvieron un efecto en las poblaciones. Rugoconites muestran agregación, posiblemente relacionado con sus métodos reproductivos en combinación con la disponibilidad de lugares de asentamiento en el momento de la dispersión y/o asentamiento. Además, los controles ambientales posteriormente al asentamiento podrían estar afectando a Rugoconites en otras superficies, lo que resultaría en poblaciones y densidades más bajas. Tanto Tribrachidium como Rugoconites ocurren como individuos en varios lechos, restringiendo las posibles estrategias reproductivas y las preferencias ambientales/de sustrato. La distribución de Obamus es consistente con un asentamiento selectivo, agregando cerca de sus congéneres y sobre sustratos de tapete microbiano maduro. Este proceso de dispersión es el primer ejemplo de dispersión selectiva de sustrato entre la biota ediacárica, lo que hace que Obamus sea similar a numerosos invertebrados sésiles modernos con estrategias similares de dispersión y asentamiento.

Phillip C. Boan↗

Turbulence measurements in a three dimensional wall jet

The effects of both a straight and a curved wall on the two-point space-time velocity correlations of a turbulent jet are documented. From the correlations, iso-correlation contours are constructed which allow a picture of the various flow patterns to be determined. Auto and cross-correlations of the fluctuating concentration field are also obtained for the different flow configurations. The confining surfaces served to radically change the flow pattern of the freely expanding turbulent jet. The velocity measurements are made with a laser Doppler velocimeter in conjunction with a phase locked-loop processor, while the concentration field is monitored with a laser light scattering technique.

Catalano, G. D.↗

The tropopause cold trap in the Australian Monsoon during STEP/AMEX 1987

The relationship between deep convection and tropopause cold trap conditions is examined for the tropical northern Australia region during the 1986-87 summer monsoon season, emphasizing the Australia Monsoon Experiment (AMEX) period when the NASA Stratosphere-Troposphere Exchange Project (STEP) was being conducted. The factors related to the spatial and temporal variability of the cold point potential temperature (CPPT) are investigated. A framework is developed for describing the relationships among surface average equivalent potential temperature in the surface layer (AEPTSL) the height of deep convection, and stratosphere-troposphere exchange. The time-mean pattern of convection, large-scale circulation, and surface AEPTSL in the Australian monsoon and the evolution of the convective environment during the monsoon period and the extended transition season which preceded it are described. The time-mean fields of cold point level variables are examined and the statistical relationships between mean CPPT, surface AEPTSL, and deep convection are described. Day-to-day variations of CPPT are examined in terms of these time mean relationships.

Selkirk, Henry B.↗

Controls of CO2 sources and sinks in the earth scale surface ocean - Temperature and nutrients

Several regions in the ocean in which disequilibrium persists on an annual avarage between CO2 in the surface water and the overlying atmosphere were examined using various models in which CO2 does cycle in a steady state at which sources (ocean outgassing) and sinks (ingassing) are in balance. The relative values of the surface temperature and surface nutrients, the two major contributors to the CO2 source and/or sink properties, are determined. Results from models with two ocean surfaces indicate that the sink in the north Atlantic and the sources in the equatorial Atlantic and Pacific are all dominated by the global temperature patterns. Results from ocean models with three surface zones show that, in the equatorial Pacific, the temperature control is responsible for over 50 percent (and, possibly, for almost 70 percent) of the CO2 outgassing, with the balance coming from the earth scale surface nutrient structure.

Volk, Tyler↗

Speckle Pattern for Woven Softgood Habitats Photogrammetry Testing

Space habitation is becoming more accessible and commercially available which is driving the development and testing of inflatable softgoods habitats that can be both lighter and more volume-efficient than heritage metallic pressure vessels. Photogrammetry test methods are used on new materials and larger inflatable softgood test articles to measure material displacement and strain during pressurization. A speckle pattern must be applied to the surface of interest for testing when using photogrammetry which is typically a very labor and time intensive process. Two methods were tested as options for a faster and more effective application to hand painting and temporary tattoos, which are the current application methods for the structural/restraint layer. One approach was to mix a plastic additive with a matte clear coat to create a speckle pattern that could be applied to the article. The second method uses a speckle paint gun to speckle the article after a white base coat is applied. Both methods adhered well to the Kevlar test sample materials and showed up well when examined with photogrammetry cameras. On the woven test samples the additive in a clear coat method could be applied evenly as was the paint gun method.

Rylee Cardon↗

Speckle Pattern Application to Woven Softgoods

Space habitation is becoming more accessible and commercially available which is driving the development and testing of inflatable softgoods habitats that can be both lighter and more volume-efficient than heritage metallic pressure vessels. Photogrammetry test methods are used on new materials and larger inflatable softgood test articles to measure material displacement and strain during pressurization. A speckle pattern must be applied to the surface of interest for testing when using photogrammetry which is typically a very labor and time intensive process. Two methods were tested as options for a faster and more effective application to hand painting and temporary tattoos, which are the current application methods for the structural/restraint layer. One approach was to mix a plastic additive with a matte clear coat to create a speckle pattern that could be applied to the article. The second method uses a speckle paint gun to speckle the article after a white base coat is applied. Both methods adhered well to the Kevlar test sample materials and showed up well when examined with photogrammetry cameras. On the woven test samples the additive in a clear coat method could be applied evenly as was the paint gun method.

Rylee Cardon↗

Speckle Pattern Application to Woven Softgoods

Space habitation is becoming more accessible and commercially available which is driving the development and testing of inflatable softgoods habitats that can be both lighter and more volume-efficient than heritage metallic pressure vessels. Photogrammetry test methods are used on new materials and larger inflatable softgood test articles to measure material displacement and strain during pressurization. A speckle pattern must be applied to the surface of interest for testing when using photogrammetry which is typically a very labor and time intensive process. Two methods were tested as options for a faster and more effective application to hand painting and temporary tattoos, which are the current application methods for the structural/restraint layer. One approach was to mix a plastic additive with a matte clear coat to create a speckle pattern that could be applied to the article. The second method uses a speckle paint gun to speckle the article after a white base coat is applied. Both methods adhered well to the Kevlar test sample materials and showed up well when examined with photogrammetry cameras. On the woven test samples the additive in a clear coat method could be applied evenly as was the paint gun method.

Rylee Cardon↗

Mars Pathfinder Landing Site: Evidence for a Change in Wind Regime from Lander and Orbiter Data

Surface features related to the wind are observed in the vicinity of the Mars Pathfinder (MPR landing site data from the lander and in data from orbit by the Viking Orbiter and Mars Global Surveyor missions. Features seen from the surface include wind tails associated with small rocks, barchanoid duneforms, ripplelike patterns, and ventifact flutes cut into some rocks. Features seen from orbit include wind tails associated with impact craters, ridges inferred to be duneforms, and modified crater rims interpreted to have been eroded and mantled by windblown material. The orientations of these features show two prevailing directions. One is inferred to represent winds from the northeast, which is consistent with strongest winds predicted by a general circulation model to occur during the Martian northern winter under current conditions. A second wind blowing from the ESE was responsible for modifying the crater rims and cutting some of the ventifacts. The two wind regimes could reflect a change in climate related to Mars' obliquity or some other, unknown factor. Regardless of the cause, the MPF area has been subjected to a complex pattern of winds and supply of small particles, and the original surface formed by sedimentary processes from Tiu and Ares Vallis flooding events has been modified by repeated burial and exhumation.

Greeley, Ronald↗

Spatially Patterned Architectures to Modulate CO 2 Reduction Cascade Catalysis Kinetics

Electrochemical CO 2 reduction using renewable sources of electrical energy holds promise for converting CO 2 into fuels and chemicals. The complex interactions among chemical/electrochemical reactions and mass transport make it difficult to analyze the effect of an individual process on electrode performance based only on experimental methods. Here, we developed a generalized steady-state simulation to describe an electrode surface in which sequential cascade catalysts are patterned in a periodic trench design. If appropriately constructed, this trench geometry is hypothesized to be able to yield a higher net current density for a CO 2 reduction (CO 2 R) cascade reaction. We have used realistic experimental reaction kinetics to investigate the role of trench geometry in mass transport, local microenvironments, and selectivity for a model CO 2 R cascade reaction. The model considers local concentration gradients of bicarbonate species at quasi-equilibrium and catalytic surface reactions based on concentration-dependent Butler–Volmer kinetics. Our results suggest that varying the spatial distribution of active sites plays a significant role in facilitating effective mass transport between active sites, modulating selectivity for the cascade reaction, and enhancing the yield of desirable cascade products. Moreover, we observe that this trench geometry significantly alters the cascade reaction rate by affecting the local pH, which can cause inadvertent depletion of available aqueous CO 2 to limit the CO 2 R cascade kinetics and modest suppression of the hydrogen evolution reaction (HER). The results highlight the trade-offs between mass transport, pH, and reaction kinetics that become apparent only when considering the coupled physics of all processes at the electrode surface. Here, this model can thus serve as a primary tool to build more selective and efficient patterned architectures for the CO 2 R cascade catalysis.

CO2 reduction↗

Multi-property modeling of ocean basin carbon fluxes

The objectives of this project were to elucidate the causal mechanisms in some of the most important features of the global ocean/atomsphere carbon system. These included the interaction of physical and biological processes in the seasonal cycle of surface water pCo2, and links between productivity, surface chlorophyll, and the carbon cycle that would aid global modeling efforts. In addition, several other areas of critical scientific interest involving links between the marine biosphere and the global carbon cycle were successfully pursued; specifically, a possible relation between phytoplankton emitted DMS and climate, and a relation between the location of calcium carbonate burial in the ocean and metamorphic source fluxes of CO2 to the atmosphere. Six published papers covering the following topics are summarized: (1) Mass extinctions, atmospheric sulphur and climatic warming at the K/T boundary; (2) Sensitivity of climate and atmospheric CO2 to deep-ocean and shallow-ocean carbonate burial; (3) Controls on CO2 sources and sinks in the earthscale surface ocean; (4) pre-anthropogenic, earthscale patterns of delta pCO2 between ocean and atmosphere; (5) Effect on atmospheric CO2 from seasonal variations in the high latitude ocean; and (6) Limitations or relating ocean surface chlorophyll to productivity.

Volk, Tyler↗

Feasibility of Sensing Tropospheric Ozone with MODIS 9.6 Micron Observations

With the infrared observations made by the Moderate Resolution Imaging Spectrometer (MODIS) on board the EOS-Aqua satellite, which include the 9.73 micron channel, a method is developed to deduce horizontal patterns of tropospheric ozone in cloud free conditions on a scale of about 100 km. It is assumed that on such small scale, at a given instant, horizontal changes in stratospheric ozone are small compared to that in the troposphere. From theoretical simulations it is found that uncertainties in the land surface emissivity and the vertical thermal stratification in the troposphere can lead to significant errors in the inferred tropospheric ozone. Because of this reason in order to derive horizontal patterns of tropospheric ozone in a given geographic area a tuning of this method is necessary with the help of a few dependent cases. After tuning, this method is applied to independent cases of MODIS data taken over Los Angeles basin in cloud free conditions to derive horizontal distribution of ozone in the troposphere. Preliminary results indicate that the derived patterns of ozone resemble crudely the patterns of surface ozone reported by EPA.

Prabhakara, C.↗

Analysis of biofilm assembly by large area automated AFM

Biofilms are complex microbial communities critical in medical, industrial, and environmental contexts. Understanding their assembly, structure, genetic regulation, interspecies interactions, and environmental responses is key to developing effective control and mitigation strategies. While atomic force microscopy (AFM) offers critically important high-resolution insights on structural and functional properties at the cellular and even sub-cellular level, its limited scan range and labor-intensive nature restricts the ability to link these smaller scale features to the functional macroscale organization of the films. We begin to address this limitation by introducing an automated large area AFM approach capable of capturing high-resolution images over millimeter-scale areas, aided by machine learning for seamless image stitching, cell detection, and classification. Large area AFM is shown to provide a very detailed view of spatial heterogeneity and cellular morphology during the early stages of biofilm formation which were previously obscured. Using this approach, we examined the organization of Pantoea sp. YR343 on PFOTS-treated glass surfaces. Our findings reveal a preferred cellular orientation among surface-attached cells, forming a distinctive honeycomb pattern. Detailed mapping of flagella interactions suggests that flagellar coordination plays a role in biofilm assembly beyond initial attachment. Additionally, we use large-area AFM to characterize surface modifications on silicon substrates, observing a significant reduction in bacterial density. This highlights the potential of this method for studying surface modifications to better understand and control bacterial adhesion and biofilm formation.

59 BASIC BIOLOGICAL SCIENCES↗