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DOE OSTI2022

A reference open-source controller for fixed and floating offshore wind turbines

Abstract. This paper describes the development of a new reference controller framework for fixed and floating offshore wind turbines that greatly facilitates controller tuning and represents standard industry practices. The reference wind turbine controllers that are most commonly cited in the literature have been developed to work with specific reference wind turbines. Although these controllers have provided standard control functionalities, they are often not easy to modify for use on other turbines, so it has been challenging for researchers to run representative, fully dynamic simulations of other wind turbine designs. The Reference Open-Source Controller (ROSCO) has been developed to provide a modular reference wind turbine controller that represents industry standards and performs comparably to or better than existing reference controllers. The formulation of the ROSCO controller logic and tuning processes is presented in this paper. Control capabilities such as tip speed ratio tracking generator torque control, minimum pitch saturation, wind speed estimation, and a smoothing algorithm at near-rated operation are included to provide modern controller features. A floating offshore wind turbine feedback module is also included to facilitate growing research in the floating offshore arena. All of the standard controller implementations and control modules are automatically tuned such that a non-controls engineer or automated optimization routine can easily improve the controller performance. This article provides the framework and theoretical basis for the ROSCO controller modules and generic tuning processes. Simulations of the National Renewable Energy Laboratory (NREL) 5 MW reference wind turbine and International Energy Agency 15 MW reference turbine on the University of Maine semisubmersible platform are analyzed to demonstrate the controller's performance in both fixed and floating configurations, respectively. The simulation results demonstrate ROSCO's peak shaving routine to reduce maximum rotor thrusts by over 10 % compared to the NREL 5 MW reference wind turbine controller on the land-based turbine and to reduce maximum platform pitch angles by nearly 30 % when using the platform feedback routine instead of a more traditional low-bandwidth controller.

17 WIND ENERGY↗

NASA NTRS2017

Design and Principles Enabling the Space Reference FOM

A first complete draft of the Simulation Interoperability Standards Organization (SISO) Space Reference Federation Object Model (FOM) has now been produced. This paper provides some insights into its capabilities and discusses the opportunity for reuse in other domains. The focus of this first version of the standard is execution control, time management and coordinate systems, well-known reference frames, as well as some basic support for physical entities. The biggest part of the execution control is the coordinated start-up process. This process contains a number of steps, including checking of required federates, handling of early versus late joiners, sharing of federation wide configuration data and multi-phase initialization. An additional part of Execution Control is the coordinated and synchronized transition between Run mode, Freeze mode and Shutdown. For time management, several time lines are defined, including real-time, scenario time, High Level Architecture (HLA) logical time and physical time. A strategy for mixing simulations that use different time steps is introduced, as well as an approach for finding common boundaries for fully synchronized freeze. For describing spatial information, a mechanism with a set of reference frames is specified. Each reference frame has a position and orientation related to a parent reference frame. This makes it possible for federates to perform calculations in reference frames that are convenient to them. An operation on the Moon can be performed using lunar coordinates whereas an operation on Earth can be performed using Earth coordinates. At the same time, coordinates in one reference frame have an unambiguous relationship to a coordinate in another reference frame. While the Space Reference FOM is originally being developed for Space operations, the authors believe that many parts of it can be reused for any simulation that has a focus on physical processes with one or more coordinate systems, and require high fidelity and repeatability.

Moeller, Bjoern↗

NASA NTRS2017

Improved Reference Sampling and Subtraction: A Technique for Reducing the Read Noise of Near-Infrared Detector Systems

Near-infrared array detectors, like the James Webb Space Telescope (JWST) NIRSpec's Teledyne's H2RGs, often provide reference pixels and a reference output. These are used to remove correlated noise. Improved reference sampling and subtraction (IRS(exp 2)) is a statistical technique for using this reference information optimally in a leastsquares sense. Compared with the traditional H2RG readout, IRS(exp 2) uses a different clocking pattern to interleave many more reference pixels into the data than is otherwise possible. Compared with standard reference correction techniques, IRS(exp 2) subtracts the reference pixels and reference output using a statistically optimized set of frequency dependent weights. The benefits include somewhat lower noise variance and much less obvious correlated noise. NIRSpec's IRS(exp 2) images are cosmetically clean, with less 1/f banding than in traditional data from the same system. This article describes the IRS(exp 2) clocking pattern and presents the equations needed to use IRS(exp 2) in systems other than NIRSpec. For NIRSpec, applying these equations is already an option in the calibration pipeline. As an aid to instrument builders, we provide our prototype IRS(exp 2) calibration software and sample JWST NIRSpec data. The same techniques are applicable to other detector systems, including those based on Teledyne's H4RG arrays. The H4RG's interleaved reference pixel readout mode is effectively one IRS(exp 2) pattern.

Methods-statistical↗

DOE OSTI2024

Statistically-driven Experimental Design to Improve Reference-free Quantification of Small Molecules by Liquid Chromatography-Mass Spectrometry

Non-targeted analysis of small molecules and metabolites in unknown, complex samples using liquid chromatography-tandem mass spectrometry remains challenging. One of the main bottlenecks is the extensive unannotated regions of metabolomics mass spectrometry data, resulting in knowledge gaps. Small molecule annotation in mass spectrometry data has conventionally relied on reference standards and libraries for compound identification and confirmation, which can constrain compound identification to those molecules already known, thus limiting the ability to discover new knowledge and new markers. Retention time prediction can facilitate and expedite unknown compound identification in non-targeted analysis of complex metabolomics samples. Additionally, accurate retention time predictions can also inform sample mixture design for LC-MS/MS analyses. However, current machine learning-based methods for retention time prediction are typically developed for specific chromatographic platforms and are not generalizable across scales. And while technologies and methods to improve reference-free metabolite identification for more comprehensive annotation of unknowns has received much attention, development of the same for quantitation without reference standards has been much more limited, despite its importance in toxicological, environmental, food safety, forensics, and clinical applications. We believe that a reference-free quantitation strategy that exploits mass spectrometry data already collected for reference-free identification can provide much more insight on unknowns, and move the metabolomics field for more complete unknowns characterization. As such, we pursue two efforts to improve upon current state-of-the-art methods in non-targeted analysis: (1) machine learning-based retention time prediction and (2) statistical design of experiments framework for reference-free quantitation. In this work, we develop and demonstrate (1) a generalizable retention time prediction capability across chromatographic conditions and scales, and (2) a statistical design-based framework for response factor contribution elucidation and reference-free quantitation. Evaluation of our retention time prediction model, PrediToR, showed approximately 24% improvement over current models, and we observed approximately 10X improvement in concentration estimation accuracy from our statistical design-based response factor model over a primarily ionization efficiency-based model. We expect that future efforts to improve upon these new capabilities will further advance non-targeted analysis of small molecules towards truly reference-free metabolomics.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2021

Improved field evaluation of reference cells using spectral measurements

Output from an IMT reference solar cell mounted on a one-axis tracking surface is investigated using spectral measurements covering the 350–1650 nm range for selected days throughout the year. Comparisons are made to a Class-A pyranometer also mounted on the one-axis tracking surface. Systematic biases over the day and year are observed in the ratio of the reference cell’s measurements to reference pyranometer measurements. This systematic bias is associated with the spectral, temperature, and angle-of-incident effects that differ between the reference cell and the reference pyranometer. The comparison is done for selected clear and totally cloudy days to determine the magnitude of the effects and to characterize the influence of these effects on the reference cell measurements. A model to calculate the reference cell output based on spectral irradiance and reference cell temperature is introduced.

14 SOLAR ENERGY↗

DOE OSTI2023

Impact of Irradiation-Induced Filter Heating on Calibration of NIR-Longpass-Filtered Reference Solar Cells

Reference solar cells play a crucial role in determining the performance of photovoltaic (PV) devices. In the performance calibration chain of mainstream single-junction PV technologies, common reference cell types include Si, KG-filtered Si, and GaAs cells. For emerging multijunction (MJ) PV technologies, such as CdTe/Si and perovskite/Si, Si reference cells with colored glass near-infrared-longpass (LP) filters like Schott RG-715 and 850 glass have been proposed. They offer a better spectral response that matches the bottom junctions of the emerging MJs, which could lead to lower uncertainties in performance measurements. However, this article reveals a prominent decrease in short-circuit current (ISC) during National Renewable Energy Laboratory's primary calibration over the course of minutes when using this type of LP-filtered reference cell, which could result in unacceptable measurement errors. Unlike quartz or KG filter glasses, LP colored glass filters demonstrate temperature-sensitive cut-on wavelength. When incident irradiance reaches these LP-filtered reference cells, the increased temperature due to light absorption causes a shift of the cut-on to longer wavelength. As a result, the device I SC exhibits a continuous decrease (approximately a 2.5% drop with RG850 LP filter in a 5-min duration) even when the device temperature is controlled at 25 degrees C. To address the temperature gradient issue between colored glass LP filter and the reference cell, we propose the direct integration of thin-film LP semiconductor layer on reference cells. This type of LP-filter-integrated cell has shown minimal temperature-related I SC variation and can serve as a more reliable reference cell source for accurate performance measurements.

14 SOLAR ENERGY↗

DOE OSTI2014

Reference Model 3 Scaled Geometry (RM3: Wave Point Absorber)

Contains the Reference Model 3 (RM3) scaled scale geometry files of the Wave Point Absorber, developed by the Reference Model Project (RMP). These scaled geometry files are saved as SolidWorks assembly, IGS, and STEP files, and require a CAD program to view. The scaled RM3 device was tested at the Scripps Institution of Oceanography at the University of California at San Diego wave tank, details of which are described in the included test report. The scale of the geometries included in this submission are at a 1:33 scale compared to the full scale geometry. This data was generated upon completion of the project on September 30, 2014. The Reference Model Project (RMP), sponsored by the U.S. Department of Energy (DOE), was a partnered effort to develop open-source MHK point designs as reference models (RMs) to benchmark MHK technology performance and costs, and an open-source methodology for design and analysis of MHK technologies, including models for estimating their capital costs, operational costs, and levelized costs of energy. The point designs also served as open-source test articles for university researchers and commercial technology developers. The RMP project team, led by Sandia National Laboratories (SNL), included a partnership between DOE, three national laboratories, including the National Renewable Energy Laboratory (NREL), Pacific Northwest National Laboratory (PNNL), and Oak Ridge National Laboratory (ORNL), the Applied Research Laboratory of Penn State University, and Re Vision Consulting. Reference Model 3 (RM3) is a wave point absorber, also referred to as a wave power buoy, that was designed for a reference site located off the shore of Eureka in Humboldt County, California. The design of the device consists of a surface float that translates (oscillates) with wave motion relative to a vertical column spar buoy, which connects to a subsurface reaction plate. This two-body point absorber converts wave energy into electrical power predominately from the device?s heave oscillation induced by incident waves; the float is designed to oscillate up and down the vertical shaft up to 4 m. The bottom of the reaction plate is about 35 m below the water surface. The device is targeted for deployment in water depths of 40 m to 100 m. The point absorber is also connected to a mooring system to keep the floating device in position.

16 TIDAL AND WAVE POWER↗

DOE OSTI2011

Reference dosimetry for helical tomotherapy: Practical implementation and a multicenter validation

Purpose: The aim of this study was to implement a protocol for reference dosimetry in tomotherapy and to validate the beam output measurements with an independent dosimetry system. Methods: Beam output was measured at the reference depth of 10 cm in water for the following three cases: (1) a 5 x 10 cm{sup 2} static machine specific reference field (MSR), (2) a rotational 5 x 10 cm{sup 2} field without modulation and no tabletop in the beam, (3) a plan class specific reference (PCSR) field defined as a rotational homogeneous dose delivery to a cylindrical shaped target volume: plan with modulation and table-top movement. The formalism for reference dosimetry of small and nonstandard fields [Med.Phys.35: 5179-5186, 2008] and QA recommendations [Med.Phys.37: 4817-4853, 2010] were adopted in the dose measurement protocol. All ionization chamber measurements were verified independently using alanine/EPR dosimetry. As a pilot study, the beam output was measured on tomotherapy Hi-art systems at three other centers and directly compared to the centers specifications and to alanine dosimetry. Results: For the four centers, the mean static output at a depth of 10 cm in water and SAD = 85 cm, measured with an A1SL chamber following the TG-148 report was 6.238 Gy/min {+-} 0.058 (1 SD); the rotational output was 6.255 Gy/min {+-} 0.069 (1 SD). The dose stated by the center was found in good agreement with the measurements of the visiting team: D{sub center}/D{sub visit} = 1.000 {+-} 0.003 (1 SD). The A1SL chamber measurements were all in good agreement with Alanine/EPR dosimetry. Going from the static reference field to the rotational/non modulated field the dose rate remains constant within 0.2% except for one center where a deviation of 1.3% was detected. Conclusions: Following the TG-148 report, beam output measurements in water at the reference depth using a local protocol, as developed at different centers, was verified. The measurements were found in good agreement with alanine/EPR dosimetry. The presented methodology may provide a good concept for reference dosimetry.

61 RADIATION PROTECTION AND DOSIMETRY↗

NASA NTRS1979

Improvement of scanning radiometer performance by digital reference averaging

Most radiometers utilize a calibration technique in which measurements of a known reference are subtracted from measurements of an unknown source so that common-mode bias errors are cancelled. When a radiometer is scanned over a varying scene, it produces a sequence of outputs, each being proportional to the difference between the reference and the corresponding input. A reference averaging technique is presented that employs a simple digital algorithm which exploits the asymmetry between the time-variable scene inputs and the nominally constant reference input by averaging many reference measurements to decrease the statistical uncertainty in the reference value. This algorithm is, therefore, optimized by an asymmetric chopping sequence in which the scene is viewed for more than one-half of the duty cycle (unlike the analog Dicke technique). Reference averaging algorithms are well within the capabilities of small microprocessors. Although this paper develops the technique for microwave radiometry, it may be beneficial for any system which measures a large number of unknowns relative to a known reference in the presence of slowly varying common-mode errors.

Bremer, J. C.↗

NASA NTRS2010

Coherent Frequency Reference System for the NASA Deep Space Network

The NASA Deep Space Network (DSN) requires state-of-the-art frequency references that are derived and distributed from very stable atomic frequency standards. A new Frequency Reference System (FRS) and Frequency Reference Distribution System (FRD) have been developed, which together replace the previous Coherent Reference Generator System (CRG). The FRS and FRD each provide new capabilities that significantly improve operability and reliability. The FRS allows for selection and switching between frequency standards, a flywheel capability (to avoid interruptions when switching frequency standards), and a frequency synthesis system (to generate standardized 5-, 10-, and 100-MHz reference signals). The FRS is powered by redundant, specially filtered, and sustainable power systems and includes a monitor and control capability for station operations to interact and control the frequency-standard selection process. The FRD receives the standardized 5-, 10-, and 100-MHz reference signals and distributes signals to distribution amplifiers in a fan out fashion to dozens of DSN users that require the highly stable reference signals. The FRD is also powered by redundant, specially filtered, and sustainable power systems. The new DSN Frequency Distribution System, which consists of the FRS and FRD systems described here, is central to all operational activities of the NASA DSN. The frequency generation and distribution system provides ultra-stable, coherent, and very low phase-noise references at 5, l0, and 100 MHz to between 60 and 100 separate users at each Deep Space Communications Complex.

Tucker, Blake C.↗

DOE OSTI2024

Cathodic Decomposition Electrodes as Standard Reference Electrodes for Molten Salts: Example of the Lithium Eutectic Electrode for the LiCl-KCl Eutectic

Alternatives to the widely-used standard anodic decomposition reference electrodes in molten salts are necessary to enable more easily reproduced thermochemical and electrochemical data in molten salt electrolytes. The class of standard reference electrodes called cathodic decomposition electrodes (CDEs) are easily constructed and can be used to make thermochemical measurements in molten salts more directly compared to anodic decomposition electrodes. The lithium eutectic electrode (LEE) was chosen as a sample test case for validation and was applied to thermochemical measurements of electroactive species in molten LiCl-KCl eutectic. Transient measurements were made to measure the Li + /Li reduction potential at zero current in pure LiCl-KCl eutectic relative to a Li-alloy reference electrode to validate the reference potential of the LEE. Literature-reported electromotive force measurements against Li-alloy reference electrodes were used to generate a relationship between the LEE and the standard chlorine electrode and this relationship was used to evaluate measured and reported formal potential measurements for the LiCl-KCl-GdCl 3 system. This work demonstrates the general framework for defining CDEs for any molten salt system and a method for calibrating external reference electrodes against a CDE standard reference electrode, improving the ease of obtaining thermochemical and electrochemical measurements in any molten salt system.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2014

Reference Model 3 Full Scale Geometry (RM3: Wave Point Absorber)

Contains the Reference Model 3 (RM3) full scale geometry files of the Wave Point Absorber, developed by the Reference Model Project (RMP). These full scale geometry files are saved as SolidWorks assembly, IGS, and STEP files, and require a CAD program to view. This data was generated upon completion of the project on September 30, 2014. The Reference Model Project (RMP), sponsored by the U.S. Department of Energy (DOE), was a partnered effort to develop open-source MHK point designs as reference models (RMs) to benchmark MHK technology performance and costs, and an open-source methodology for design and analysis of MHK technologies, including models for estimating their capital costs, operational costs, and levelized costs of energy. The point designs also served as open-source test articles for university researchers and commercial technology developers. The RMP project team, led by Sandia National Laboratories (SNL), included a partnership between DOE, three national laboratories, including the National Renewable Energy Laboratory (NREL), Pacific Northwest National Laboratory (PNNL), and Oak Ridge National Laboratory (ORNL), the Applied Research Laboratory of Penn State University, and Re Vision Consulting. Reference Model 3 (RM3) is a wave point absorber, also referred to as a wave power buoy, that was designed for a reference site located off the shore of Eureka in Humboldt County, California. The design of the device consists of a surface float that translates (oscillates) with wave motion relative to a vertical column spar buoy, which connects to a subsurface reaction plate. This two-body point absorber converts wave energy into electrical power predominately from the device's heave oscillation induced by incident waves; the float is designed to oscillate up and down the vertical shaft up to 4 m. The bottom of the reaction plate is about 35 m below the water surface. The device is targeted for deployment in water depths of 40 m to 100 m. The point absorber is also connected to a mooring system to keep the floating device in position.

16 TIDAL AND WAVE POWER↗

DOE OSTI2021

PV Reference Cells for Outdoor Use: An Investigation of Calibration Factors

Reference cells are widely used in the photovoltaic (PV) industry to measure irradiance. For field applications and outdoor use, a variety of products are on the market, and they are often perceived as a low-cost alternative to thermopile radiometers. But reference cell characteristics differ substantially from the latter, therefore measurements made by one category of instrument cannot be substituted for or directly compared with the other. The National Renewable Energy Laboratory's (NREL's) Solar Radiation Research Laboratory is currently in a multiyear effort to develop guidance and recommendations for the design and use of outdoor reference cells, with a view to reducing inconsistencies and measurement uncertainty. The core of this effort is the long-term deployment of 39 units of 10 distinct types made by 6 manufacturers. This report compares the calibration factors provided by manufacturers - which most users rely upon - to those measured at a high level of accuracy by NREL's Cell Lab. It also reports on the application of the Broadband Outdoor Radiometer Calibration (BORCAL) method to reference cells. As a group, the factory calibrations for the crystalline silicon cells showed a small positive bias of +0.7%, with a tight distribution around this value. This is a very good result for the manufacturers. The two World Photovoltaic Scale cells constituted outliers, with a mean deviation of -0.8%, which is still within the reported uncertainty ranges. The data collected during the BORCAL procedures show a strong and systematic fluctuation of the apparent responsivity of the reference cells over the course of a day, which was anticipated. Consequently, the standard BORCAL R@45 calibration factors underestimated the reference cell responsivities by 1%-4%. An alternate responsivity indicator was developed, R'@30, incorporating adjustments for temperature response, spectral response, and directional response. This reduced the mean deviation to -0.4% compared to the Cell Lab. Further, two independent BORCAL sessions were able to produce the same values to within ±0.2%. This could be a viable approach for verifying reference cells using existing BORCAL infrastructure, for example, to evaluate their long-term stability. The daily profiles of responsivity obtained during the BORCAL sessions (provided in Appendix A) show clear patterns of differences between models, which provides evidence that their directional responses differ from each other; thus, two sensors that are calibrated correctly might give the same reading at 30° zenith angle but systematically different readings at 60°. The impact of these inconsistencies in field operation will be quantified using the long-term observations started in 2020.

14 SOLAR ENERGY↗

DOE OSTI2025

Land-Based Wind Reference Architecture

This report describes a summary and the final deliverables for the Wind Reference Architecture project funded by the Department of Energy's (DOE) Wind Energy Technology Office (WETO). The project objective was to further refine the reference architecture of the existing wind power plant reference architecture developed by Idaho National Laboratory (INL) and Sandia National Laboratories (SNL) by expanding the wind turbine generator (WTG) into a separate individual wind turbine reference architecture. Additional details regarding the wind turbine system and how it integrates with a wind power plant were researched and reflected in the reference architecture. This addition is important because it allows researchers to understand the components and devices in a wind turbine, how they function and how a wind turbine integrates into a wind power plant to be able to perform cybersecurity evaluations on the system. The communication and control systems were the focus while developing this reference architecture to understand the cybersecurity posture of a wind turbine and power plant. The information technology (IT) and operational technology (OT) systems perspective are integral in helping improve the cybersecurity posture by creating a starting point to study how wind energy can be safely designed and deployed in our power grid from an integrated systems standpoint. A holistic wind power plant and turbine reference architecture and simulation was developed and made open-sourced for further research efforts.

17 WIND ENERGY↗

DOE OSTI2014

Reference Model 2 Scaled Geometry (RM2: River Current Turbine)

Contains the Reference Model 2 (RM2) scaled scale geometry files of the River Current Turbine, developed by the Reference Model Project (RMP). These scaled geometry files are saved as SolidWorks assembly, IGS, and STEP files, and require a CAD program to view. The scaled RM2 device was tested at the Saint Anthony Falls Laboratory (SAFL) at the University of Minnesota flume. The scale of the geometries included in this submission are at a 1:15 scale compared to the full scale geometry. This data was generated upon completion of the project on September 30, 2014. The Reference Model Project (RMP), sponsored by the U.S. Department of Energy (DOE), was a partnered effort to develop open-source MHK point designs as reference models (RMs) to benchmark MHK technology performance and costs, and an open-source methodology for design and analysis of MHK technologies, including models for estimating their capital costs, operational costs, and levelized costs of energy. The point designs also served as open-source test articles for university researchers and commercial technology developers. The RMP project team, led by Sandia National Laboratories (SNL), included a partnership between DOE, three national laboratories, including the National Renewable Energy Laboratory (NREL), Pacific Northwest National Laboratory (PNNL), and Oak Ridge National Laboratory (ORNL), the Applied Research Laboratory of Penn State University, and Re Vision Consulting. Reference Model 2 (RM2) is a variable speed dual-rotor cross-flow river turbine that is deployed at the water?s surface. It was designed for deployment at a reference site modeled after a reach in the Mississippi River near Baton Rouge, Louisiana. The rotors are anchored to a two-pontoon vessel platform. Surface deployment of the turbine minimizes the handling requirements during deployment and recovery and reduces overall costs for all O&M activities, including allowing for easy access to the power conversion chain (PCC). The design (two rotors per platform) also reduces the environmental footprint and associated environmental compliance costs.

16 TIDAL AND WAVE POWER↗

DOE OSTI2014

Reference Model 3 Cost Breakdown (RM3: Wave Point Absorber)

Contains the Reference Model 3 (RM3) spreadsheets with the cost breakdown structure (CBS) for the levelized cost of energy (LCOE) calculations for a single RM3 device and multiple unit arrays. These spreadsheets are contained within an XLSX file and a spreadsheet editor such as Microsoft Excel is needed to open the file. This data was generated upon completion of the project on September 30, 2014. The Reference Model Project (RMP), sponsored by the U.S. Department of Energy (DOE), was a partnered effort to develop open-source MHK point designs as reference models (RMs) to benchmark MHK technology performance and costs, and an open-source methodology for design and analysis of MHK technologies, including models for estimating their capital costs, operational costs, and levelized costs of energy. The point designs also served as open-source test articles for university researchers and commercial technology developers. The RMP project team, led by Sandia National Laboratories (SNL), included a partnership between DOE, three national laboratories, including the National Renewable Energy Laboratory (NREL), Pacific Northwest National Laboratory (PNNL), and Oak Ridge National Laboratory (ORNL), the Applied Research Laboratory of Penn State University, and Re Vision Consulting. Reference Model 3 (RM3) is a wave point absorber, also referred to as a wave power buoy, that was designed for a reference site located off the shore of Eureka in Humboldt County, California. The design of the device consists of a surface float that translates (oscillates) with wave motion relative to a vertical column spar buoy, which connects to a subsurface reaction plate. This two-body point absorber converts wave energy into electrical power predominately from the devices heave oscillation induced by incident waves; the float is designed to oscillate up and down the vertical shaft up to 4 m. The bottom of the reaction plate is about 35 m below the water surface. The device is targeted for deployment in water depths of 40 m to 100 m. The point absorber is also connected to a mooring system to keep the floating device in position.

16 TIDAL AND WAVE POWER↗

DOE OSTI2018

Validation of reference genes for real-time PCR of cord blood mononuclear cells, differentiating endothelial progenitor cells, and mature endothelial cells

Highlights: • RT-qPCR was used to assess and follow the differentiation of EPCs. • A set of reliable and non-variable genes is key to obtain accurate RT-qPCR results. • The relative gene expression of CD34+ and differentiating endothelial progenitor cells was determined. • The optimal set of reference for the gene expression studies performed by RT-qPCR was determined. In the last ten years, endothelial progenitor cells (EPCs) have gained interest as an attractive cell population in regenerative medicine for vascular applications. This population is defined as the precursor of endothelial mature cells (ECs) through a process of differentiation. To our knowledge, no single marker can be used to discriminate them from mature ECs. To effectively study their differentiation kinetics, gene expression must be assessed. Quantitative real-time PCR (RT-qPCR) is widely used to analyze gene expression. To minimize the impact of variances from RT-qPCR, a rigorous selection of reference genes must be performed prior to any experiments due to variations in experimental conditions. In this study, CD34+ mononuclear cells were extracted from human cord blood and differentiated into EPCs after seeding for a maximum period of 21 days. To choose the best combinations of reference genes, we compared the results of EPCs, CD34+ mononuclear cells, and mature endothelial cells to ensure that the differentiation kinetics did not affect the expression of our selected reference genes. The expression levels of seven genes, namely, YWHAZ, GAPDH, HPRT1, RPLP0, UBC, B2M, and TBP were thus compared. The algorithms geNorm, NormFinder, BestKeeper, and the Comparative ΔCt method were employed to assess the expression of each candidate gene. Overall results reveal that the expression stability of reference genes may differ depending on the statistical program used. YWHAZ, GAPDH, and UBC composed the optimal set of reference genes for the gene expression studies performed by RT-qPCR in our experimental conditions. This work can thus serve as a starting point for the selection of candidate reference genes to normalize the levels of gene expression in endothelial progenitor cell populations.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2017

Self-Supporting, Hydrophobic, Ionic Liquid-Based Reference Electrodes Prepared by Polymerization-Induced Microphase Separation

Interfaces of ionic liquids and aqueous solutions exhibit stable electrical potentials over a wide range of aqueous electrolyte concentrations. This makes ionic liquids suitable as bridge materials that separate in electroanalytical measurements the reference electrode from samples with low and/or unknown ionic strengths. However, methods for the preparation of ionic liquid-based reference electrodes have not been explored widely. We have designed a convenient and reliable synthesis of ionic liquid-based reference electrodes by polymerization-induced microphase separation. This technique allows for a facile, single-pot synthesis of ready-to-use reference electrodes that incorporate ion conducting nanochannels filled with either 1-octyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide or 1-dodecyl-3-methylimidazolium bis(trifluoromethyl sulfonyl)imide as ionic liquid, supported by a mechanically robust cross-linked polystyrene phase. This synthesis procedure allows for the straightforward design of various reference electrode geometries. These reference electrodes exhibit a low resistance as well as good reference potential stability and reproducibility when immersed into aqueous solutions varying from deionized, purified water to 100 mM KCl, while requiring no correction for liquid junction potentials.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗