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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 145 records · Page 8

Variations in chloroplast movement and chlorophyll fluorescence among chloroplast division mutants under light stress

Chloroplasts divide to maintain consistent size, shape, and number in leaf mesophyll cells. Altered expression of chloroplast division proteins in Arabidopsis results in abnormal chloroplast morphology. To better understand the influence of chloroplast morphology on chloroplast movement and photosynthesis, we compared the chloroplast photorelocation and photosynthetic responses of a series of Arabidopsis chloroplast division mutants with a wide variety of chloroplast phenotypes. Chloroplast movement was monitored by red light reflectance imaging of whole plants under increasing intensities of white light. The accumulation and avoidance responses were differentially affected in different mutants and depended on both chloroplast number and morphological heterogeneity. Chlorophyll fluorescence measurements during 5 d light experiments demonstrated that mutants with large-chloroplast phenotypes generally exhibited greater PSII photodamage than those with intermediate phenotypes. No abnormalities in photorelocation efficiency or photosynthetic capacity were observed in plants with small-chloroplast phenotypes. Simultaneous measurement of chloroplast movement and chlorophyll fluorescence indicated that the energy-dependent (qE) and long-lived components of non-photochemical quenching that reflect photoinhibition are affected differentially in different division mutants exposed to high or fluctuating light intensities. We conclude that chloroplast division mutants with abnormal chloroplast morphologies differ markedly from the wild type in their light adaptation capabilities, which may decrease their relative fitness in nature.

59 BASIC BIOLOGICAL SCIENCES↗

Modeling Data Movement Performance on Heterogeneous Architectures

The cost of data movement on parallel systems varies greatly with machine architecture, job partition, and nearby jobs. Performance models that accurately capture the cost of data movement provide a tool for analysis, allowing for communication bottlenecks to be pinpointed. Modern heterogeneous architectures yield increased variance in data movement as there are a number of viable paths for inter-GPU communication. In this paper, we present performance models for the various paths of inter-node communication on modern heterogeneous architectures, including the trade-off between GPUDirect communication and copying to CPUs. Furthermore, we present a novel optimization for inter-node communication based on these models, utilizing all available CPU cores per node. Finally, we show associated performance improvements for MPI collective operations.

97 MATHEMATICS AND COMPUTING↗

Movement through Dams Facilitates Population Connectivity in a Large River

Even in heavily impounded river ecosystems, aquatic populations and communities retain limited connectivity via movement through dams. This connectivity has the potential to influence population dynamics but has been infrequently characterized. We used 1995–2008 paddlefish mark–recapture data to perform the following: (i) quantify rates of movement through dams and (ii) examine the influence of dam discharge on fish passing dams. We found that there are substantial one-way (upstream to downstream) population connections maintained via fish passing dams and that dam discharge is a key driver of downstream fish movement. Results of our study suggest that population connections maintained by fish passing dams can play an important role in population dynamics depleting upstream populations and subsidizing downstream populations, particularly in years with high flow events. We suggest that the influence of hydrology on maintaining populations in fragmented ecosystems is an increasingly important consideration for conservation and management of aquatic ecosystems in the face of predicted hydrological changes from climate change.

Pracheil, Brenda↗

Enterprise Cyber Resiliency Against Lateral Movement: A Graph Theoretic Approach

Lateral movement attacks are a serious threat to enterprise security. In these attacks, an attacker compromises a trusted user account to get a foothold into the enterprise network and uses it to attack other trusted users, increasingly gaining higher and higher privileges. Such lateral attacks are very hard to model because of the unwitting role that users play in the attack and even harder to detect and prevent because of their low and slow nature. In this paper, a theoretical framework is presented for modeling lateral movement attacks and for proposing a methodology for designing resilient cyber systems against such attacks. The enterprise is modeled as a tripartite graph capturing the interaction between users, machines, and applications, and a set of procedures is proposed to harden the network by increasing the cost of lateral movement. Strong theoretical guarantees on system resilience are established and experimentally validated for large enterprise networks.

Chen, Pin-Yu↗

Optimizing Data Movement for GPU-Based In-Situ Workflow Using GPUDirect RDMA

The extreme-scale computing landscape is increasingly dominated by GPU-accelerated systems. At the same time, in-situ workflows that employ memory-to-memory inter-application data exchanges have emerged as an effective approach for leveraging these extreme-scale systems. In the case of GPUs, GPUDirect RDMA enables third-party devices, such as network interface cards, to access GPU memory directly and has been adopted for intra-application communications across GPUs. In this paper, we present an interoperable framework for GPU-based in-situ workflows that optimizes data movement using GPUDirect RDMA. Specifically, we analyze the characteristics of the possible data movement pathways between GPUs from an in-situ workflow perspective, and design a strategy that maximizes throughput. Furthermore, we implement this approach as an extension of the DataSpaces data staging service, and experimentally evaluate its performance and scalability on a current leadership GPU cluster. The performance results show that the proposed design reduces data-movement time by up to 53% and 40% for the sender and receiver, respectively, and maintains excellent scalability for up to 256 GPUs.

Zhang, Bo↗

Characterizing the movements and habitat use of two fish species of concern in a regulated ecosystem

In freshwater ecosystems in the northwest United States, the distribution and movements of fish between their essential habitats are particularly impacted by the presence of hydroelectric dams. Bull Trout Salvelinus confluentus and Westslope Cutthroat Trout Oncorhynchus clarkii var. lewisi are two fish species of concern inhabiting the Pend Oreille River between Idaho and Washington states. The purpose of this study was to identify the behavior and habitat use of Bull Trout and Westslope Cutthroat Trout in the restricted area of the Pend Oreille River, downstream of Albeni Falls Dam (AFD), using a long-term radiotelemetry dataset. Between 2015 and 2018, 24 Bull Trout and 24 Westslope Cutthroat Trout were captured, tagged, and released downstream AFD. Among the fish tagged, 79% (n = 19 of 24) of Bull Trout and 67% (n = 16 of 24) of Westslope Cutthroat Trout were detected during the study period. Our results revealed movements of fish towards tributary confluences mostly in May, June, and July for both species with Bull Trout traveling longer distances than Westslope Cutthroat Trout. Although the sample size was limited, Bull Trout individual movements towards the dam suggested attempts to re-ascend the river, indicating the importance of restoring upstream connectivity.

Garavelli, Lysel↗

PAK4 crystal structures suggest unusual kinase conformational movements

In order for protein kinases to exchange nucleotide they must open and close their catalytic cleft. These motions are associated with rotations of the N-lobe, predominantly around the ‘hinge region’. Here, we conducted an analysis of 28 crystal structures of the serine-threonine kinase, p21-activated kinase 4 (PAK4), including three newly determined structures in complex with staurosporine, FRAX486, and fasudil (HA-1077). We find an unusual motion between the N-lobe and C-lobe of PAK4 that manifests as a partial unwinding of helix αC. Principal component analysis of the crystal structures rationalizes these movements into three major states, and analysis of the kinase hydrophobic spines indicates concerted movements that create an accessible back pocket cavity. The conformational changes that we observe for PAK4 differ from previous descriptions of kinase motions, and although we observe these differences in crystal structures there is the possibility that the movements observed may suggest a diversity of kinase conformational changes associated with regulation. Author summary: Protein kinases are key signaling proteins, and are important drug targets, therefore understanding their regulation is important for both basic research and clinical points of view. In this study, we observe unusual conformational ‘hinging’ for protein kinases. Hinging, the opening and closing of the kinase sub-domains to allow nucleotide binding and release, is critical for proper kinase regulation and for targeted drug discovery. We determine new crystal structures of PAK4, an important Rho-effector kinase, and conduct analyses of these and previously determined structures. We find that PAK4 crystal structures can be classified into specific conformational groups, and that these groups are associated with previously unobserved hinging motions and an unusual conformation for the kinase hydrophobic core. Our findings therefore indicate that there may be a diversity of kinase hinging motions, and that these may indicate different mechanisms of regulation.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

GBNV encoded movement protein (NSm) remodels ER network via C-terminal coiled coil domain

Plant viruses exploit the host machinery for targeting the viral genome–movement protein complex to plasmodesmata (PD). The mechanism by which the non-structural protein m (NSm) of Groundnut bud necrosis virus (GBNV) is targeted to PD was investigated using Agrobacterium mediated transient expression of NSm and its fusion proteins in Nicotiana benthamiana. GFP:NSm formed punctuate structures that colocalized with mCherry:plasmodesmata localized protein 1a (PDLP 1a) confirming that GBNV NSm localizes to PD. Unlike in other movement proteins, the C-terminal coiled coil domain of GBNV NSm was shown to be involved in the localization of NSm to PD, as deletion of this domain resulted in the cytoplasmic localization of NSm. Treatment with Brefeldin A demonstrated the role of ER in targeting GFP NSm to PD. Furthermore, mCherry:NSm co-localized with ER–GFP (endoplasmic reticulum targeting peptide (HDEL peptide fused with GFP). Co-expression of NSm with ER–GFP showed that the ER-network was transformed into vesicles indicating that NSm interacts with ER and remodels it. Mutations in the conserved hydrophobic region of NSm (residues 130–138) did not abolish the formation of vesicles. Additionally, the conserved prolines at positions 140 and 142 were found to be essential for targeting the vesicles to the cell membrane. Further, systematic deletion of amino acid residues from N- and C-terminus demonstrated that N-terminal 203 amino acids are dispensable for the vesicle formation. On the other hand, the C-terminal coiled coil domain when expressed alone could also form vesicles. These results suggest that GBNV NSm remodels the ER network by forming vesicles via its interaction through the C-terminal coiled coil domain. Interestingly, NSm interacts with NP in vitro and coexpression of these two proteins in planta resulted in the relocalization of NP to PD and this relocalization was abolished when the N-terminal unfolded region of NSm was deleted. Thus, the NSm interacts with NP via its N-terminal unfolded region and the NSm–NP complex could in turn interact with the ER membrane via the C-terminal coiled coil domain of NSm to form vesicles that are targeted to PD and there by assist the cell to cell movement of the viral genome complex. - Highlights: • GBNV NSm localizes to plasmodesmata via the C-terminal coiled coil domain. • GBNV NSm interacts with endoplasmic reticulum network and remodels it to vesicles. • The C-terminal coiled domain alone is responsible for vesicle formation. • The N-terminal unfolded region of NSm is involved in the re-localization of NP to PD.

60 APPLIED LIFE SCIENCES↗

Linking Small-Scale Flight Manoeuvers and Density Profiles to the Vertical Movement of Insects in the Nocturnal Stable Boundary Layer

Huge numbers of insects migrate over considerable distances in the stably-stratified night-time atmosphere with great consequences for ecological processes, biodiversity, ecosystem services and pest management. We used a combination of meteorological radar and lidar instrumentation at a site in Oklahoma, USA, to take a new look at the general assistance migrants receive from both vertical and horizontal airstreams during their long-distance flights. Movement in the nocturnal boundary layer (NBL) presents very different challenges for migrants compared to those prevailing in the daytime convective boundary layer, but we found that Lagrangian stochastic modelling is effective at predicting flight manoeuvers in both cases. A key feature for insect transport in the NBL is the frequent formation of a thin layer of fast-moving air – the low-level jet. Modelling suggests that insects can react rapidly to counteract vertical air movements and this mechanism explains how migrants are retained in the jet for long periods (e.g. overnight, and perhaps for several hours early in the morning). This results in movements over much longer distances than are likely in convective conditions, and is particularly significant for the reintroduction of pests to northern regions where they are seasonally absent due to low winter temperatures.

54 ENVIRONMENTAL SCIENCES↗

Metallographic autopsies of full-scale ITER prototype cable-in-conduit conductors after full cyclic testing in SULTAN: II. Significant reduction of strand movement and strand damage in short twist pitch CICCs

Prototype cable in conduit conductors (CICCs) destined for use in the Toroidal Field (TF) and Central Solenoid (CS) coils of the ITER experimental fusion reactor underwent severe cyclic loading in the SULTAN facility. Their autopsies revealed significant and permanent transverse strand migration due to the large Lorentz forces of the SULTAN test. The movement resulted in a 3 7% void fraction increase on the Low Pressure (LP) side of the longer twist pitch CICCs. However, short twist pitch conductors exhibited less than 1% void fraction increase in the LP side, as well as a complete absence of the Nb 3 Sn filament fractures observed in the longer twist pitch conductors. We report here a detailed strand to cable analysis of short and longer “baseline” twist pitch CICCs. It was found that the use of Internal Tin strands in the longer “baseline” twist pitch CICCs can be beneficial possibly because of their superior stiffness—which better resist strand movement—while the use of Bronze Process strands showed more movement and poorer cyclic test performance. This was not the case for the short twist pitch CICC. Such conductor design seems to work well with both strand types. But it was found that despite the absence of filament fractures, the short twist pitch CICC made from the Internal Tin strands studied here developed severe strand distortion during cabling which resulted in diffusion barrier breaks and Sn contamination of the Cu stabilizer during the heat treatment. Furthermore, the short twist pitch CICC made from Bronze Process strands preserved diffusion barrier integrity.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Assimilating electronic tagging, oceanographic modelling, and fisheries data to estimate movements and connectivity of swordfish in the North Atlantic

Abstract Broadbill swordfish (Xiphias gladius) is a key species in commercial fisheries, but management efforts continue to be hindered by data gaps in their basic biology, including ongoing debate over stock boundaries and movement between management zones. We deployed 20 pop-up satellite archival transmitting (PSAT) tags on juvenile swordfish in the Azores and adults in the northwest Atlantic (NWA). We applied a recently developed geolocation approach that synthesizes tag data with a global observation-assimilating ocean model to improve geolocation accuracy. Resulting tracks from 16 individuals averaged 3751 km (range 345–7247 km) over 93 days (range 17–181 days). Juveniles tagged in the Azores made regional movements while adults tagged in the NWA moved between summer foraging grounds near the Grand Banks to winter habitats near the Antillean Arc. All individuals spent considerable time in the mesopelagic zone during the day, and this behaviour increased with fish size. We integrate results from our PSAT-based movements with conventional tag and catch-per-unit effort data, which indicates complex stock structure within the North Atlantic that largely supports current stock boundaries. Our work demonstrates the utility in synthesizing fishery-independent electronic tag data and fisheries datasets to improve our understanding of large pelagic fish ecology.

Braun, Camrin D.↗

Fast Vehicle Turning-Movement Counting using Localization-based Tracking

Despite the high utility of traffic volume and turning movement data, such data is still hard to come by for the vast majority of roadways and intersections in nearly ev- ery city. Edge computing devices offer a promising tool for recording turning movement data if lightweight algorithms can be designed to run in real-time with relatively modest computational complexity. To that end, this work presents Vehicle Turning-Movement Counting using Localization- based Tracking (LBT-Count). This method is fast because it never performs detection on a full frame. Instead, only a few portions of the image are cropped and used to de- tect objects within the frame. The method achieves com- petitive performance on the public evaluation server for Track 1 of the AI City Challenge (7th overall on the first 50% of data). Furthermore, we show that LBT-Count is 52% faster than an analogous counting algorithm utilizing a traditional tracking-by-detection framework on available challenge data.

42 ENGINEERING↗

Improved Localization Precision and Angular Resolution of a Cylindrical, Time-Encoded Imaging System From Adaptive Detector Movements

To the first order, the localization precision and angular resolution of a cylindrical, time-encoded imaging (c-TEI) system is governed by the geometry of the system. Improving either measure requires increasing the mask radius or decreasing the detector diameter, both of which are undesirable. Here, we propose an alternative option of repositioning the detector within the mask to increase the detector-to-mask distance in the direction of a source, thereby improving the localization precision and angular resolution in that direction. Since the detector-to-mask distance only increases for a small portion of the field of view (FOV), we propose implementing adaptive imaging where one leverages data collected during the measurement to optimize the system configuration. This article utilizes both simulations and experiments to set upper bounds on the potential gain from adaptive detector movements for one and two sources in the FOV. When only one source is present, adaptive detector movements can improve the localization precision and angular resolution by 20% for a source at 90 cm and by 32% for a far-field source. When two sources are present, adaptive detector movements can improve localization precision and angular resolution by up to 50% for sources that are ~10° apart (90 cm from the system). We experimentally verify these results through maximum likelihood estimation of the source position(s) and image reconstruction of point sources that are close together. As a demonstration of an adaptive imaging algorithm, we image a complex arrangement of special nuclear material at the Zero Power Physics Reactor facility at Idaho National Laboratory.

46 - INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AN↗

The flagella of ‘ Candidatus Liberibacter asiaticus’ and its movement in planta

Summary Citrus huanglongbing (HLB) is the most devastating citrus disease worldwide. ‘ Candidatus Liberibacter asiaticus’ (Las) is the most prevalent HLB causal agent that is yet to be cultured. Here, we analysed the flagellar genes of Las and Rhizobiaceae and observed two characteristics unique to the flagellar proteins of Las: (i) a shorter primary structure of the rod capping protein FlgJ than other Rhizobiaceae bacteria and (ii) Las contains only one flagellin‐encoding gene flaA (CLIBASIA_02090), whereas other Rhizobiaceae species carry at least three flagellin‐encoding genes. Only flgJ Atu but not flgJ Las restored the swimming motility of Agrobacterium tumefaciens flgJ mutant. Pull‐down assays demonstrated that FlgJ Las interacts with FlgB but not with FliE. Ectopic expression of flaA Las in A. tumefaciens mutants restored the swimming motility of ∆ flaA mutant and ∆ flaAD mutant, but not that of the null mutant ∆ flaABCD . No flagellum was observed for Las in citrus and dodder. The expression of flagellar genes was higher in psyllids than in planta . In addition, western blotting using flagellin‐specific antibody indicates that Las expresses flagellin protein in psyllids, but not in planta . The flagellar features of Las in planta suggest that Las movement in the phloem is not mediated by flagella. We also characterized the movement of Las after psyllid transmission into young flush. Our data support a model that Las remains inside young flush after psyllid transmission and before the flush matures. The delayed movement of Las out of young flush after psyllid transmission provides opportunities for targeted treatment of young flush for HLB control.

Andrade, Maxuel O.↗

Movement of the Melt Metal Layer under Conditions Typical of Transient Events in ITER

During the operation of ITER, protective coatings of the divertor and the first wall will be exposed to significant plasma heat loads which may cause a huge erosion. One of the major failure mechanisms of metallic armor is diminution of their thickness due to the melt layer displacement. New experimental data are required in order to develop and validate physical models of the melt layer movement. The paper presents the experiments where metal targets were irradiated by a plasma stream at the quasi-stationary high-current plasma accelerator QSPA-T. The obtained data allow one to determine the velocity and acceleration of the melt layer at various distances from the plasma stream axis. The force causing the radial movement of the melt layer is shown to create an acceleration whose order of magnitude is 1000g. The pressure gradient is not responsible for creating this large acceleration. To investigate the melt layer movement under a known force, the experiment with a rotating target was carried out. The influence of centrifugal and Coriolis forces led to appearance of curved elongated waves on the surface. The surface profile changed: there is no hill in the central part of the erosion crater in contrast to the stationary target. The experimental data clarify the trends in the melt motion that are required for development of theoretical models.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

qSIEVE: Efficient qLDPC Memory via Systolic Movement in Atom Arrays

As quantum machines have scaled up in their number of qubits, significant research has turned towards increasing their fidelity with quantum error correction codes. Although promising results have been shown with the surface code, which only requires near-neighbor connections between qubits, the high qubit overhead of such local codes promises to be problematic. Consequently, recent work has explored non-local quantum LDPC (qLDPC) codes, which have good asymptotic encoding rates. Despite theoretical progress, hardware implementations of these codes have been a longstanding challenge. At the experimental level, demonstrations of movement based communication on atom arrays suggest this is a powerful new primitive to achieve non-local connectivity. Leveraging this, we present a protocol for implementing non-local qLDPC codes in hardware. Our protocol, qSIEVE, is a co-design of such codes with movement in atom arrays. qSIEVE defines a restricted family of qLDPC codes that can be implemented efficiently with systolic movement. We then quantify the utility of qSIEVE in the context of a complete fault tolerant architecture. We compare the cost of implementing benchmark programs in a standard, surface code only architecture and a mixed architecture where data is stored in qLDPC memory with qSIEVE and loaded to surface codes for computation.

Quantum error correction↗

Impacts of electromagnetic fields associated with marine and hydrokinetic surrogate technologies on fish movements and behaviors.

Marine and hydrokinetic energy (MHK) and offshore wind devices are being developed and deployed in U.S. and international waters. Electric current flowing through subsea transmission cables associated with these devices will generate electromagnetic fields (EMF), which may interact with, and potentially impact, marine fishes. Some marine fishes can detect electric and/or magnetic fields and use them to navigate, orientate, and sense prey, mates and predators. Over the past five years there have been multiple comprehensive reviews and studies evaluating the potential vulnerability of marine fishes to EMF produced by MHK devices. Most documented effects involve sub-lethal behavioral responses of individual fish when in close proximity to EMF (e.g., fish being repelled by or attracted to fields). These reviews reach conclusions that the current state of research on this topic is still in its infancy and evaluations of potential impacts are associated with great uncertainty. A variety of MHK technologies are likely to be considered for deployment offshore of the Hawaiian Islands, and there is a need to be able to better predict and assess potential associated environmental impacts. The goal of this study was to provide a complementary piece to these previous reviews (e.g., Normandeau et al. 2011) by focusing on marine fish species in the Hawaii region. We compiled the relevant available information, then prioritized fish species as candidates for various paths of future research. To address this, we first developed a list of Hawaii Region Focal Species, which included fishes that are more likely to be sensitive to EMF. We then compiled species-specific information available in the literature on their sensitivity to EMF, as well as life history, movement and habitat use information that could inform an analysis of their likelihood of encountering EMF from subsea cables associated with MHK devices. Studies have only documented EMF sensitivity in 11 of the marine fish species in this region. There was also relatively little detailed information on fish movement and habitat use patterns for most of the focal species. Our last objective was to develop recommendations for research needs to close the important knowledge gaps. We describe species-independent baseline research that primarily consists of in situ quantification of EMF generated by MHK devices and undersea cables that can occur as pilot and commercial scale MHK devices are deployed in Hawaii. Then we propose a simple approach for prioritizing Hawaii Region Focal Species (ranked relative to each other) as candidates in multiple related research paths. The prioritization approach incorporates EMF sensitivity information with the likelihood of interacting with EMF generated undersea transmission cables associated with MHK devices. Finally, we discuss the types of research needed to help fill gaps in the scientific knowledge base for this region. These involve studies to better define species-specific EMF sensitivity thresholds under various environmental conditions, studies of life history, movement and habitat use patterns to improve our understanding of the likelihood and frequency fishes may be in the vicinity of EMF generated by subsea transmission cables, and studies of the potential for related population, community or ecosystem impacts. Many of these studies can and should occur opportunistically as pilot and commercial scale MHK devices are deployed in Hawaii.

59 BASIC BIOLOGICAL SCIENCES↗

Influence of synoptic atmospheric conditions on movement of individual sea-ice floes in Fram Strait, late summer 2010

Abstract In this paper we investigate the effect on sea-ice movement of changes in the synoptic atmospheric conditions in late boreal summer 2010. Our study area is the western Fram Strait, a crucial passage for the transport of ice out of the Arctic basin. Ice dynamics here affect the movement of ice in the East Greenland Current, the transpolar drift and ice extent in the Arctic Ocean. In contrast to other times of the year, when the Fram Strait wind field is characterized by strong, persistent northerlies, we show that the weaker, more variable winds typical during late summer for the Fram Strait can slow movement of ice floes out of the area, thus slowing the export of ice from the Arctic Ocean at the end of summer, a time crucial for ice export. The Arctic Ocean could lose even more of the ice that survives the summer if this was not the case. This would leave the Arctic Ocean in an even more vulnerable position with regard to the amount of multi-year ice remaining the following summer.

Geology↗