Dominant negative mutations of Arabidopsis RWA
The present invention provides for dominant negative mutations of Arabidopsis REDUCING WALL ACETYLATION (RWA).
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The present invention provides for dominant negative mutations of Arabidopsis REDUCING WALL ACETYLATION (RWA).
The Cassini spacecraft was launched on October 15, 1997 and arrived at Saturn on June 30, 2004. It has performed detailed observations and remote sensing of Saturn, its rings, and its satellites since that time. Cassini deployed the European-built Huygens probe, which descended through the Titan atmosphere (Saturn's largest moon) and landed on its surface on January 14, 2005. The Cassini mission has recently been approved by NASA to continue through September of 2017. This 7-year extension is called the Solstice mission and it presents challenges to the spacecraft operations team and its ability to maintain the health of the spacecraft. To keep the spacecraft healthy for 7 more years, the spacecraft team must carefully manage hydrazine use (about 48% of the 132 kg launch load remains as of January 2011). A vital part of conserving hydrazine is to use the reaction wheel assembly (RWA) control system for precise pointing and slews wherever possible. In any given week, the Cassini spacecraft is commanded to use RWA control about 99% of the time, with about 1% of the time requiring reaction control system (RCS) thruster control (to perform Delta V course corrections or to bias the RWA momentum). Such extensive use of the RWA hardware throughout the mission requires that the RWAs be operated in a way that minimizes degradation in the RWA electronics, DC motor, and spin bearing for each reaction wheel. Three consumables in particular have been identified for the RWAs: (1) Total number of revolutions for each RWA. (2) Time spent at very low wheel speeds. At these low speeds, good elasto-hydrodynamic (EHD) film lubrication may be compromised. (3) Total number of on/off power cycles. The second of these consumables, minimizing the time spent at very low wheel speeds, is especially important to keep the spin bearing healthy and well-lubricated. These consumables are actively managed by the attitude control operations team throughout the mission. One vital management technique is to predict individual RWA momentum (given the pointing and slews that are needed to collect the best science) and to bias the RWA momentum in a way that reduces both the total number of revolutions as well as the time spent below EHD wheel speed. Another strategy to protect RWA health is to alter the planned pointing of the spacecraft (which can affect science collection) so that the RWA consumables are conserved. This paper focuses on why this second technique is needed, and discusses how guidelines have been developed by the attitude control team which affects the planned science pointing, so that science data can be most optimally collected while still minimizing RWA consumable usage.
This study determines the feasibility of creating a tool that can accurately predict Lunar Reconnaissance Orbiter (LRO) reaction wheel assembly (RWA) angular momentum, weeks or even months into the future. LRO is a three-axis stabilized spacecraft that was launched on June 18, 2009. While typically nadir-pointing, LRO conducts many types of slews to enable novel science collection. Momentum unloads have historically been performed approximately once every two weeks with the goal of maintaining system total angular momentum below 70 Nms; however flight experience shows the models developed before launch are overly conservative, with many momentum unloads being performed before system angular momentum surpasses 50 Nms. A more accurate model of RWA angular momentum growth would improve momentum unload scheduling and decrease the frequency of these unloads. Since some LRO instruments must be deactivated during momentum unloads and in the case of one instrument, decontaminated for 24 hours there after a decrease in the frequency of unloads increases science collection. This study develops a new model to predict LRO RWA angular momentum. Regression analysis of data from October 2014 to October 2015 was used to develop relationships between solar beta angle, slew specifications, and RWA angular momentum growth. The resulting model predicts RWA angular momentum using input solar beta angle and mission schedule data. This model was used to predict RWA angular momentum from October 2013 to October 2014. Predictions agree well with telemetry; of the 23 momentum unloads performed from October 2013 to October 2014, the mean and median magnitude of the RWA total angular momentum prediction error at the time of the momentum unloads were 3.7 and 2.7 Nms, respectively. The magnitude of the largest RWA total angular momentum prediction error was 10.6 Nms. Development of a tool that uses the models presented herein is currently underway.
This paper presents a modeling methodology used to predict the performance of a flexible structure, such as a space telescope, in the presence of an on-board vibrational disturbance source, such as a reaction wheel assembly (RWA). Both decoupled and coupled analysis methods are presented. The decoupled method relies on blocked RWA disturbances, measured with the RWA hardmounted to a rigid surface. The coupled method corrects the blocked RWA disturbance boundary conditions using "force filters," which depend on estimates of the interface accelerances of the RWA and space-craft. Both methods were validated on the Micro-Precision Interferometer testbed at the Jet Propulsion Laboratory. Experimental results are encouraging, indicating that both methods provide sufficient accuracy compared to measured values; however, the coupled method provides the best results when the gyroscopic nature of the spinning RWA is captured in the RWA accelerance model. Additionally, the RWA disturbance cross spectral density terms are found to be influential.
A simple fourth-order propagator [Ture and Jang, J. Phys. Chem. A 128, 2871 (2024)] based on the Magnus expansion is extended to the Liouville space for both closed-system and Lindbladian open-system quantum dynamics. For both dynamics, commutator free versions of fourth-order propagators are provided as well. These propagators are then applied to the dynamics of a driven Λ-system, where Lindblad terms represent the effect of a photonic bath. For both dynamics, the accuracy of the rotating wave approximation (RWA) for the matter–radiation interaction is assessed. We confirmed reasonable performance of RWA for weak and resonant fields. However, small errors appear for moderate fields and substantial errors can be found for strong fields where coherent population trapping can still be expected. We also found that the presence of bath for open-system quantum dynamics consistently reduces the errors of the RWA. These results provide quantitative information on how the RWA breaks down beyond weak field or for non-resonant cases. Major results are benchmarked against results of our sixth-order ME-based propagator. Finally, we also provide numerical comparison of our algorithms with other fourth-order algorithms for the Λ-system. These confirm reasonable performance of our simple propagators and the improvement gained through commutator-free expressions.
The Lunar Reconnaissance Orbiter (LRO) makes use of four Reaction Wheel Assemblies (RWA) for the purpose of adjusting and maintaining the spacecraft attitude. Reduction of RWA angular momentum through the use of propulsive momentum unloads is the principal source of fuel use. Nominally, LRO maintains a nadir orientation but frequently slews off-nadir for science observations. Off-nadir attitude slews can reduce RWA angular momentum by changing the direction of the external torques in the spacecraft body frame. Control of RWA angular momentum using momentum attitude slews has the potential to significantly reduce fuel expenditure. The momentum slew planning process requires careful evaluation of spacecraft constraints, ground constraints, and science observations. Momentum slews are projected to extend LRO fuel lifetime by several years.
Emitted forces and moments characteristics of the Space Telescope Reaction Wheel Assembly (ST RWA) were measured under room temperature and pressure, thermal extremes, and vibratory conditions. The RWA/Emitted Vibration Measurement Fixture was calibrated statically and dynamically, and background noise was measured with ST RWA not operating. A base line set of forces and moments of the ST RWA along and about three mutually perpendicular axes were recorded at room ambient. The temperature vibration sensitivites shown are those which were concluded to be a function of rotor unbalance changes and not associated with either spin motor nor rotor electronic changes.
Software was developed to characterize the drag in each of the Cassini spacecraft's Reaction Wheel Assemblies (RWAs) to determine the RWA friction parameters. This tool measures the drag torque of RWAs for not only the high spin rates (greater than 250 RPM), but also the low spin rates (less than 250 RPM) where there is a lack of an elastohydrodynamic boundary layer in the bearings. RWA rate and drag torque profiles as functions of time are collected via telemetry once every 4 seconds and once every 8 seconds, respectively. Intermediate processing steps single-out the coast-down regions. A nonlinear model for the drag torque as a function of RWA spin rate is incorporated in order to characterize the low spin rate regime. The tool then uses a nonlinear parameter optimization algorithm based on the Nelder-Mead simplex method to determine the viscous coefficient, the Dahl friction, and the two parameters that account for the low spin-rate behavior.
On November 4, 2010 the already "in-flight" Deep Impact spacecraft flew within 700km of comet 103P/Hartley 2 as part of its extended mission EPOXI, the 5th time to date any spacecraft visited a comet. In 2005, the spacecraft had previously imaged a probe impact comet Tempel 1. The EPOXI flyby marked the first time in history that two comets were explored with the same instruments on a re-used spacecraft-with hardware and software originally designed and optimized for a different mission. This made the function of the attitude determination and control subsystem (ADCS) critical to the successful execution of the EPOXI flyby. As part of the spacecraft team preparations, the ADCS team had to perform thorough sequence reviews, key spacecraft activities and onboard calibrations. These activities included: review of background sequences for the initial conditions vector, sun sensor coefficients, and reaction wheel assembly (RWA) de-saturations; design and execution of 10 trajectory correction maneuvers; science calibration of the two telescope instruments; a flight demonstration of the fastest turns conducted by the spacecraft between Earth and comet point; and assessment of RWA health (given RWA problems on other spacecraft).
The Geostationary Operational Environmental Satellite-R Series (GOES-R) is the first of the next generation geostationary weather satellites, scheduled for delivery in late 2015. GOES-R represents a quantum increase in Earth and solar weather observation capabilities, with 4 times the resolution, 5 times the observation rate, and 3 times the number of spectral bands for Earth observations. With the improved resolution, comes the instrument suite's increased sensitive to disturbances over a broad spectrum 0-512 Hz. Sources of disturbance include reaction wheels, thruster firings for station keeping and momentum management, gimbal motion, and internal instrument disturbances. To minimize the impact of these disturbances, the baseline design includes an Earth Pointed Platform (EPP), a stiff optical bench to which the two nadir pointed instruments are collocated together with the Guidance Navigation & Control (GN&C) star trackers and Inertial Measurement Units (IMUs). The EPP is passively isolated from the spacecraft bus with Honeywell D-Strut isolators providing attenuation for frequencies above approximately 5 Hz in all six degrees-of-freedom. A change in Reaction Wheel Assembly (RWA) vendors occurred very late in the program. To reduce the risk of RWA disturbances impacting performance, a secondary passive isolation system manufactured by Moog CSA Engineering was incorporated under each of the six 160 Nms RWAs, tuned to provide attenuation at frequencies above approximately 50 Hz. Integrated wheel and isolator testing was performed on a Kistler table at NASA Goddard Space Flight Center. High fidelity simulations were conducted to evaluate jitter performance for four topologies: 1) hard mounted no isolation, 2) EPP isolation only, 2) RWA isolation only, and 4) dual isolation. Simulation results demonstrate excellent performance relative to the pointing stability requirements, with dual isolated Line of Sight (LOS) jitter less than 1 micron rad.
The Wide Field Infrared Survey Telescope (WFIRST) is a NASA observatory with two scientific instruments. The first is the Wide Field Instrument (WFI) designed to perform wide field imaging and surveys of the near infrared (NIR) sky. The second is a coronagraph (CGI) that will enable astronomers to detect and measure properties of planets in other solar systems. The coronagraph requires 0.5 milli-arcsecond (mas) RMS pointing per axis of the line of sight (LOS) to achieve a contrast of 1x10-9. This paper discusses the approach used for achieving this level of pointing performance on the occulting mask coronagraph (OMC) testbed at JPL. This testbed uses a low-order wavefront sensing (LOWFS) camera and fast steering mirror (FSM) to suppress injected LOS jitter and environmental LOS jitter. The injected jitter includes representative broadband spacecraft attitude control system (ACS) LOS motion and tonal LOS jitter caused by the reaction wheel assemblies (RWA). The environmental jitter includes thermal LOS variations and harmonics of the line noise. The LOWFS camera uses high flux from the obscured science target to achieve high rate measurements of the LOS. These measurements are augmented with fictitious RWA tachometer information and an estimate of the line noise frequency. A LOS servo using a combination of feedback and feedforward control is used in the testbed to compensate for all of the disturbance sources and to mitigate jitter caused by in-band camera noise. The feedforward uses a novel robust least mean squares (RLMS) filter algorithm to reject the RWA and line frequency tones. High fidelity models of the sensors, disturbances and actuator are presented in this paper. These models were developed as part of a simulation of the LOS control system. Performance results from the hardware testbed at the Jet Propulsion Laboratory (JPL) are discussed in this paper.
Abstract Plant cell wall polysaccharides, including xylan, glucomannan, xyloglucan and pectin, are often acetylated. Although a number of acetyltransferases responsible for the acetylation of some of these polysaccharides have been biochemically characterized, little is known about the source of acetyl donors and how acetyl donors are translocated into the Golgi, where these polysaccharides are synthesized. In this report, we investigated roles of ATP-citrate lyase (ACL) that generates cytosolic acetyl-CoA in cell wall polysaccharide acetylation and effects of simultaneous mutations of four Reduced Wall Acetylation (RWA) genes on acetyl-CoA transport into the Golgi in Arabidopsis thaliana. Expression analyses of genes involved in the generation of acetyl-CoA in different subcellular compartments showed that the expression of several ACL genes responsible for cytosolic acetyl-CoA synthesis was elevated in interfascicular fiber cells and induced by secondary wall-associated transcriptional activators. Simultaneous downregulation of the expression of ACL genes was demonstrated to result in a substantial decrease in the degree of xylan acetylation and a severe alteration in secondary wall structure in xylem vessels. In addition, the degree of acetylation of other cell wall polysaccharides, including glucomannan, xyloglucan and pectin, was also reduced. Moreover, Golgi-enriched membrane vesicles isolated from the rwa1/2/3/4 quadruple mutant were found to exhibit a drastic reduction in acetyl-CoA transport activity compared with the wild type. These findings indicate that cytosolic acetyl-CoA generated by ACL is essential for cell wall polysaccharide acetylation and RWAs are required for its transport from the cytosol into the Golgi.
Diagrams of the Reaction Wheel Assembly (RWA) are presented along with charts and graphs illustrating jitter error model, induced vibration tests, radial displacement transfer function, and axial displacement power spectra density. The RWA isolator specification requirements are listed.
In the event of a failure of one of MAP's three reaction wheel assemblies (RWAs), it is not possible to achieve three-axis, full-state attitude control using the remaining two wheels. Hence, two of the attitude control algorithms implemented on the MAP spacecraft will no longer be usable in their current forms: Inertial Mode, used for slewing to and holding inertial attitudes, and Observing Mode, which implements the nominal dual-spin science mode. This paper describes the effort to create a complete strategy for using software algorithms to cope with a RWA failure. The discussion of the design process will be divided into three main subtopics: performing orbit maneuvers to reach and maintain an orbit about the second Earth-Sun libration point in the event of a RWA failure, completing the mission using a momentum-bias two-wheel science mode, and developing a new thruster-based mode for adjusting the inertially fixed momentum bias. In this summary, the philosophies used in designing these changes is shown; the full paper will supplement these with algorithm descriptions and testing results.
A document discusses the concept of a demisable motor-drive-and-flywheel assembly [reaction-wheel assembly (RWA)] used in controlling the attitude of a spacecraft. Demisable as used here does not have its traditional legal meaning; instead, it signifies susceptible to melting, vaporizing, and/or otherwise disintegrating during re-entry of the spacecraft into the atmosphere of the Earth so as not to pose a hazard to anyone or anything on the ground. Prior RWAs include parts made of metals (e.g., iron, steel, and titanium) that melt at high temperatures and include structures of generally closed character that shield some parts (e.g., magnets) against re-entry heating. In a demisable RWA, the flywheel would be made of aluminum, which melts at a lower temperature. The flywheel web would not be a solid disk but would have a more open, nearly-spoke-like structure so that it would disintegrate more rapidly; hence, the flywheel rim would separate more rapidly so that parts shielded by the rim would be exposed sooner to re-entry heating. In addition, clearances between the flywheel and other components would be made greater, imparting a more open character and thus increasing the exposure of those components.
RBOT [RWA Bias Optimization Tool (wherein RWA signifies Reaction Wheel Assembly )] is a computer program designed for computing angular momentum biases for reaction wheels used for providing spacecraft pointing in various directions as required for scientific observations. RBOT is currently deployed to support the Cassini mission to prevent operation of reaction wheels at unsafely high speeds while minimizing time in undesirable low-speed range, where elasto-hydrodynamic lubrication films in bearings become ineffective, leading to premature bearing failure. The problem is formulated as a constrained optimization problem in which maximum wheel speed limit is a hard constraint and a cost functional that increases as speed decreases below a low-speed threshold. The optimization problem is solved using a parametric search routine known as the Nelder-Mead simplex algorithm. To increase computational efficiency for extended operation involving large quantity of data, the algorithm is designed to (1) use large time increments during intervals when spacecraft attitudes or rates of rotation are nearly stationary, (2) use sinusoidal-approximation sampling to model repeated long periods of Earth-point rolling maneuvers to reduce computational loads, and (3) utilize an efficient equation to obtain wheel-rate profiles as functions of initial wheel biases based on conservation of angular momentum (in an inertial frame) using pre-computed terms.
A momentum management tool was designed for the Dawn low-thrust interplanetary spacecraft en route to the asteroids Vesta and Ceres, in an effort to better understand the early creation of the solar system. Momentum must be managed to ensure the spacecraft has enough control authority to perform necessary turns and hold a fixed inertial attitude against external torques. Along with torques from solar pressure and gravity-gradients, ion-propulsion engines produce a torque about the thrust axis that must be countered by the four reaction wheel assemblies (RWA). MomProf is a ground operations tool built to address these concerns. The momentum management tool was developed during initial checkout and early cruise, and has been refined to accommodate a wide range of momentum-management issues. With every activity or sequence, wheel speeds and momentum state must be checked to avoid undesirable conditions and use of consumables. MomProf was developed to operate in the MATLAB environment. All data are loaded into MATLAB as a structure to provide consistent access to all inputs by individual functions within the tool. Used in its most basic application, the Dawn momentum tool uses the basic principle of angular momentum conservation, computing momentum in the body frame, and RWA wheel speeds, for all given orientations in the input file. MomProf was designed specifically to be able to handle the changing external torques and frequent de - saturations. Incorporating significant external torques adds complexity since there are various external torques that act under different operational modes.
A paper describes attitude-control algorithms using the combination of magnetic actuators with reaction wheel assemblies (RWAs) or other types of actuators such as thrusters. The combination of magnetic actuators with one or two RWAs aligned with different body axis expands the two-dimensional control torque to three-dimensional. The algorithms can guarantee the spacecraft attitude and rates to track the commanded attitude precisely. A design example is presented for nadir-pointing, pitch, and yaw maneuvers. The results show that precise attitude tracking can be reached and the attitude- control accuracy is comparable with RWA-based attitude control. When there are only one or two workable RWAs due to RWA failures, the attitude-control system can switch to the control algorithms for the combined magnetic actuators with the RWAs without going to the safe mode, and the control accuracy can be maintained. The attitude-control algorithms of the combined actuators are derived, which can guarantee the spacecraft attitude and rates to track the commanded values precisely. Results show that precise attitude tracking can be reached, and the attitude-control accuracy is comparable with 3-axis wheel control.