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Maintaining molecular cleanliness during the JWST’s Optical Telescope/Instrument Module (OTIS) Cryogenic Thermal Vacuum (TV) test campaign was critical to the success of its optical mission on orbit. In the thermal vacuum tests leading up to the final cryogenic test to validate the OTIS flight hardware, NASA Johnson Space Center’s (JSC’s) TV Chamber A was fully characterized for molecular contamination. It was found to contain common volatile condensable materials (VCM), including hydrocarbons, plasticizers, and silicones, all of which absorb in JWST’s infrared wavelength region. Due to the risks involved, cleaning molecular contamination from the OTIS mirrors was not an option and heating the Primary Mirror (PM) segments would have also been a risky and expensive endeavor. As a result, a monitoring process was developed and implemented during four different Pathfinder or risk reduction tests that were scheduled to occur prior to the flight hardware test. The goal was to quantify and assess the risk of molecular contamination depositing on the PM resulting from relatively warm chamber shrouds “leading” colder PM mirrors during warmup, by a margin of 10-50 Kelvin (K). This was accomplished using Cryogenic Quartz Crystal Microbalances (CQCMs), held at temperatures slightly cooler than the segments to signal the onset of contamination events. Per the JWST Contamination Control Plan (CCP)1, the total Primary Mirror molecular allocation requirement was 50 angstroms. In all tests, the results showed an average accumulated molecular contamination of <10 angstroms.