SEM visualization of glycosylated surface molecules using lectin-coated microspheres

Duke, J.; Janer, L.; Campbell, M.

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NASA NTRS · 20040089339

SEM visualization of glycosylated surface molecules using lectin-coated microspheres

Duke, J.·Janer, L.·Campbell, M.

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Abstract

There are several techniques currently used to localize glycosylated surface molecules by scanning electron microscopy (Grinnell, 1980; Molday, 1976; Linthicum and Sell, 1975; Nicolson, 1974; Lo Buglio, et al, 1972). A simple and rapid method, using a modification of Grinnell's technique is reported here. Essentially, microspheres coated with Concavalin A are used to bind to glycosylated regions of the palatal shelf epithelium and are visualized in the scanning electron microscope (SEM).

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Non-NASA Center NASA Discipline Plant Biology Concanavalin A/analysis/chemistry Lectins/analysis/chemistry Microscopy, Electron, Scanning/methods Membrane Proteins/analysis/metabolism/ultrastructure Mice Palate/chemistry/cytology/metabolism/ultrastructure Animals Mice, Inbred ICR Glycosylation Microspheres Epithelium/chemistry/metabolism/ultrastructure Epithelial Cells

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Duke, J., Janer, L., Campbell, M.. 1985-01-01. SEM visualization of glycosylated surface molecules using lectin-coated microspheres. https://ntrs.nasa.gov/citations/20040089339

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