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DOE OSTI · 1769227

Facilitating enzymatic hydrolysis with a novel guaiacol-based deep eutectic solvent pretreatment

Huang, Chen·Zhan, Yunni·Cheng, Jinyuan·Wang, Jia·Meng, Xianzhi·Zhou, Xuelian·Fang, Guigan·Ragauskas, Arthur J.
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Abstract

In this work, we established a novel deep eutectic solvent (DES) using lignin-derived guaiacol as hydrogen bond donor (HBD). The sole ChCl/guaiacol system was found to be inefficient for the fractionation of wheat straw (WS), while the incorporation of trace AlCl 3 significantly facilitated the degradation of hemicellulose and lignin, resulting in a complete enzymatic digestibility of the pretreated WS. Further, this study revealed that the DES-degraded lignin was readily precipitated during the washing process, and thus hindered the enzymatic hydrolysis of poplar and bamboo (with hydrolysis yield of 42.03% and 71.67%, respectively). Alkali washing offers a possible approach to remove the precipitated lignin, after which a near 100% hydrolysis yield was also obtained for poplar and bamboo.

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59 BASIC BIOLOGICAL SCIENCESDeep eutectic solventEnzymatic hydrolysisGuaiacol

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Huang, Chen, Zhan, Yunni, Cheng, Jinyuan, Wang, Jia, Meng, Xianzhi, Zhou, Xuelian, Fang, Guigan, Ragauskas, Arthur J.. 2021-01-12. Facilitating enzymatic hydrolysis with a novel guaiacol-based deep eutectic solvent pretreatment. https://doi.org/10.1016/j.biortech.2021.124696

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